Pdpk1 3-phosphoinositide dependent protein kinase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pdpk1provided by MGI
Official Full Name: 3-phosphoinositide dependent protein kinase 1provided by MGI
Primary source: MGI:MGI:1338068
See related: Ensembl:ENSMUSG00000024122 AllianceGenome:MGI:1338068
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pdk1
Summary: Enables 3-phosphoinositide-dependent protein kinase activity. Involved in several processes, including cell surface receptor protein tyrosine kinase signaling pathway; negative regulation of cardiac muscle cell apoptotic process; and type B pancreatic cell development. Acts upstream of or within hyperosmotic response. Located in cytoplasmic vesicle and postsynaptic density. Is expressed in several structures, including central nervous system; early conceptus; gonad; hemolymphoid system gland; and retina. Human ortholog(s) of this gene implicated in prostate cancer. Orthologous to several human genes including PDPK1 (3-phosphoinositide dependent protein kinase 1). [provided by Alliance of Genome Resources, Nov 2024]
Annotation information: Note: Pdk1 (Gene ID: 228026) and Pdpk1 (Gene ID: 18607) share the Pdk1 symbol/alias in common. Pdk1 is a widely used alternative name for 3-phosphoinositide dependent protein kinase 1 (Pdpk1), which can be confused with the official symbol for pyruvate dehydrogenase kinase, isoenzyme 1 (Pdk1). [03 Apr 2019]
Expression: Ubiquitous expression in testis adult (RPKM 17.0), thymus adult (RPKM 9.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 12.23 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (24292654..24370957, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (24073680..24155619, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The effect of PDK1 in maintaining immune cell development and function.
Title: The effect of PDK1 in maintaining immune cell development and function.
Deletion of PDK1 Caused Cardiac Malmorphogenesis and Heart Defects Due to Profound Protein Phosphorylation Changes Mediated by SHP2.
Title: Deletion of PDK(1) Caused Cardiac Malmorphogenesis and Heart Defects Due to Profound Protein Phosphorylation Changes Mediated by SHP(2).
Silencing METTL3 Stabilizes Atherosclerotic Plaques by Regulating the Phenotypic Transformation of Vascular Smooth Muscle Cells via the miR-375-3p/PDK1 Axis.
Title: Silencing METTL3 Stabilizes Atherosclerotic Plaques by Regulating the Phenotypic Transformation of Vascular Smooth Muscle Cells via the miR-375-3p/PDK1 Axis.
Extracellular vesicle-derived circCEBPZOS attenuates postmyocardial infarction remodeling by promoting angiogenesis via the miR-1178-3p/PDPK1 axis.
Title: Extracellular vesicle-derived circCEBPZOS attenuates postmyocardial infarction remodeling by promoting angiogenesis via the miR-1178-3p/PDPK1 axis.
PDK1 Regulates the Lengthening of G1 Phase to Balance RGC Proliferation and Differentiation during Cortical Neurogenesis.
Title: PDK1 Regulates the Lengthening of G1 Phase to Balance RGC Proliferation and Differentiation during Cortical Neurogenesis.
TMEM116 is required for lung cancer cell motility and metastasis through PDK1 signaling pathway.
Title: TMEM116 is required for lung cancer cell motility and metastasis through PDK1 signaling pathway.
MicroRNA-193b impairs muscle growth in mouse models of type 2 diabetes by targeting the PDK1/Akt signalling pathway.
Title: MicroRNA-193b impairs muscle growth in mouse models of type 2 diabetes by targeting the PDK1/Akt signalling pathway.
A PDK-1 allosteric agonist neutralizes insulin signaling derangements and beta-amyloid toxicity in neuronal cells and in vitro.
Title: A PDK-1 allosteric agonist neutralizes insulin signaling derangements and beta-amyloid toxicity in neuronal cells and in vitro.
The kinase PDK1 regulates regulatory T cell survival via controlling redox homeostasis.
Title: The kinase PDK1 regulates regulatory T cell survival via controlling redox homeostasis.
Deletion of PDK1 in oligodendrocyte lineage cells causes white matter abnormality and myelination defect in the central nervous system.
Title: Deletion of PDK1 in oligodendrocyte lineage cells causes white matter abnormality and myelination defect in the central nervous system.

Submit: New GeneRIF Correction See all GeneRIFs (142)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1) 1 citation
Gene trapped (2)
Targeted (11) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC175797P1 (e-PCR)
- UniSTS:271638

NoName (e-PCR)
- UniSTS:237590

Pdpk1 (e-PCR), detects polymorphism
- UniSTS:532427

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3-phosphoinositide-dependent protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 3-phosphoinositide-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables insulin receptor binding	ISO Inferred from Sequence Orthology more info
enables phospholipase activator activity	IEA Inferred from Electronic Annotation more info
enables phospholipase activator activity	ISO Inferred from Sequence Orthology more info
enables phospholipase binding	IEA Inferred from Electronic Annotation more info
enables phospholipase binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in calcium-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in cell migration	IEA Inferred from Electronic Annotation more info
involved_in cellular response to epidermal growth factor stimulus	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in epidermal growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in extrinsic apoptotic signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in focal adhesion assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within hyperosmotic response	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in insulin-like growth factor receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin-like growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cardiac muscle cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of endothelial cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of toll-like receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of blood vessel endothelial cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein localization to plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of release of sequestered calcium ion into cytosol	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of sprouting angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of vascular endothelial cell proliferation	IEA Inferred from Electronic Annotation more info
involved_in regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of endothelial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mast cell degranulation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	TAS Traceable Author Statement more info	PubMed
involved_in type B pancreatic cell development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in perikaryon	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 3-phosphoinositide-dependent protein kinase 1

Names: Pkb kinase

NP_001074242.1

EC 2.7.11.1

NP_001273591.1

EC 2.7.11.1

NP_035192.2

EC 2.7.11.1

XP_017172809.1

EC 2.7.11.1

XP_017172810.1

EC 2.7.11.1

XP_017172811.1

EC 2.7.11.1

XP_017172813.1

EC 2.7.11.1

XP_017172814.1

EC 2.7.11.1

XP_017172815.1

EC 2.7.11.1

XP_030105434.1

EC 2.7.11.1

XP_030105435.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001080773.2 → NP_001074242.1 3-phosphoinositide-dependent protein kinase 1 isoform B

See identical proteins and their annotated locations for NP_001074242.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate splice site in the 3' region, compared to variant 1. The resulting isoform (B) is shorter and has a distinct C-terminus, compared to isoform A.

Source sequence(s)

AK162677, BQ175596, BY257456

Consensus CDS

CCDS37483.1

UniProtKB/TrEMBL

Q3TRL2, Q3UGN6, Q3UHZ0

Related

ENSMUSP00000111070.2, ENSMUST00000115411.8

Conserved Domains (3) summary

smart00220
Location:85 → 345

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd01262
Location:444 → 521

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:83 → 345

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1
NM_001286662.1 → NP_001273591.1 3-phosphoinositide-dependent protein kinase 1 isoform C

Status: VALIDATED

Description

Transcript Variant: This variant (3) has an alternate 5' exon, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (C) has a shorter N-terminus, compared to isoform A.

Source sequence(s)

AK147835, AK149162, CJ046953, CT010502

Consensus CDS

CCDS70767.1

UniProtKB/TrEMBL

F2Z3X6, Q8K3L3

Related

ENSMUSP00000061942.7, ENSMUST00000052462.13

Conserved Domains (2) summary

cd01262
Location:417 → 523

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:56 → 318

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1
NM_011062.4 → NP_035192.2 3-phosphoinositide-dependent protein kinase 1 isoform A

See identical proteins and their annotated locations for NP_035192.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (A).

Source sequence(s)

AK147149, AK147835, BQ175596, BY257456, CT010502

Consensus CDS

CCDS28474.1

UniProtKB/Swiss-Prot

A6H6U3, Q9R1D8, Q9R215, Q9Z2A0

UniProtKB/TrEMBL

Q3UHZ0

Related

ENSMUSP00000099991.4, ENSMUST00000102927.10

Conserved Domains (3) summary

smart00220
Location:85 → 345

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd01262
Location:444 → 550

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:83 → 345

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

24292654..24370957 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017317324.3 → XP_017172813.1 3-phosphoinositide-dependent protein kinase 1 isoform X2

Conserved Domains (2) summary

cd01262
Location:283 → 389

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cl21453
Location:47 → 184

PKc_like; Protein Kinases, catalytic domain
XM_030249574.2 → XP_030105434.1 3-phosphoinositide-dependent protein kinase 1 isoform X3

Conserved Domains (2) summary

cd01262
Location:225 → 331

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cl21453
Location:1 → 126

PKc_like; Protein Kinases, catalytic domain
XM_017317326.3 → XP_017172815.1 3-phosphoinositide-dependent protein kinase 1 isoform X1

UniProtKB/TrEMBL

F2Z3X6, Q8K3L3

Conserved Domains (2) summary

cd01262
Location:417 → 523

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:56 → 318

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1
XM_017317325.3 → XP_017172814.1 3-phosphoinositide-dependent protein kinase 1 isoform X2

Conserved Domains (2) summary

cd01262
Location:283 → 389

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cl21453
Location:47 → 184

PKc_like; Protein Kinases, catalytic domain
XM_030249575.2 → XP_030105435.1 3-phosphoinositide-dependent protein kinase 1 isoform X3

Conserved Domains (2) summary

cd01262
Location:225 → 331

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cl21453
Location:1 → 126

PKc_like; Protein Kinases, catalytic domain
XM_017317321.3 → XP_017172810.1 3-phosphoinositide-dependent protein kinase 1 isoform X1

UniProtKB/TrEMBL

F2Z3X6, Q8K3L3

Conserved Domains (2) summary

cd01262
Location:417 → 523

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:56 → 318

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1
XM_017317320.3 → XP_017172809.1 3-phosphoinositide-dependent protein kinase 1 isoform X1

UniProtKB/TrEMBL

F2Z3X6, Q8K3L3

Conserved Domains (2) summary

cd01262
Location:417 → 523

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:56 → 318

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1
XM_017317322.1 → XP_017172811.1 3-phosphoinositide-dependent protein kinase 1 isoform X1

UniProtKB/TrEMBL

F2Z3X6, Q8K3L3

Conserved Domains (2) summary

cd01262
Location:417 → 523

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:56 → 318

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1