Ttk Ttk protein kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ttkprovided by MGI
Official Full Name: Ttk protein kinaseprovided by MGI
Primary source: MGI:MGI:1194921
See related: Ensembl:ENSMUSG00000038379 AllianceGenome:MGI:1194921
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PYT; esk; Esk1; Mps1
Summary: Enables identical protein binding activity; kinetochore binding activity; and protein serine/threonine/tyrosine kinase activity. Involved in mitotic spindle assembly checkpoint signaling. Acts upstream of or within several processes, including meiotic spindle assembly checkpoint signaling; protein localization to organelle; and protein phosphorylation. Located in kinetochore. Is expressed in several structures, including brain; lung; and metanephros. Orthologous to human TTK (TTK protein kinase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in CNS E11.5 (RPKM 9.2), placenta adult (RPKM 7.3) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 E2; 9 45.61 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (83716704..83754442)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (83834651..83872390)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The essential roles of Mps1 in spermatogenesis and fertility in mice.
Title: The essential roles of Mps1 in spermatogenesis and fertility in mice.
using in vitro dephosphorylation assays, we demonstrate that Mastl promotes persistent MPS1 phosphorylation by inhibiting PP2A/B55-mediated MPS1 dephosphorylation rather than affecting Cdk1 kinase activity. Our findings establish a key regulatory function of the Greatwall kinase/Mastl - PP2A/B55 pathway in preventing premature SAC silencing
Title: Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint.
In a sensitized p53(+/-) background, Mps1(DK/DK) mice suffered from rapid-onset acute lymphoblastic lymphoma.
Title: Chromosome instability induced by Mps1 and p53 mutation generates aggressive lymphomas exhibiting aneuploidy-induced stress.
Mps1 is essential for meiosis I in mouse. Kinetochore localization of Mps1 is required for correct timing of prometaphase, to prevent precocious APC/C activation, for SAC control, correct aurora C (Aurkc) localization and chromosome alignment.
Title: Mps1 at kinetochores is essential for female mouse meiosis I.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH125325 (e-PCR)
- UniSTS:162147

M86377 (e-PCR)
- UniSTS:163464

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables histone H2AXY142 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3Y41 kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinetochore binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine/tyrosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine/tyrosine kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within female meiosis chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meiotic prometaphase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within meiotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within peptidyl-threonine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein localization to chromosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to kinetochore	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein localization to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization to meiotic spindle midzone	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in repair of mitotic kinetochore microtubule attachment defect	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: dual specificity protein kinase TTK

NP_001103735.1

EC 2.7.12.1

NP_001271201.1

EC 2.7.12.1

NP_033471.2

EC 2.7.12.1

XP_006511121.1

EC 2.7.12.1

XP_006511123.1

EC 2.7.12.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110265.2 → NP_001103735.1 dual specificity protein kinase TTK isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) includes a 3' terminal exon that extends beyond a splice site used in variant 1 and results in a distinct 3' coding region and 3' UTR compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC124011

UniProtKB/TrEMBL

Q8BY97

Related

ENSMUST00000188445.7

Conserved Domains (3) summary

cd14131
Location:496 → 764

PKc_Mps1; Catalytic domain of the Dual-specificity Mitotic checkpoint protein kinase, Monopolar spindle 1 (also called TTK)

sd00006
Location:105 → 134

TPR; TPR repeat [structural motif]

pfam00069
Location:498 → 764

Pkinase; Protein kinase domain
NM_001284272.2 → NP_001271201.1 dual specificity protein kinase TTK isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an exon and uses an alternate splice site in the 3' coding region compared to variant 1. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 1.

Source sequence(s)

AC124011

Consensus CDS

CCDS81044.1

UniProtKB/TrEMBL

Q3TPW2, Q6PD96

Related

ENSMUSP00000139956.2, ENSMUST00000185913.7

Conserved Domains (3) summary

sd00006
Location:105 → 134

TPR; TPR repeat [structural motif]

pfam00069
Location:498 → 707

Pkinase; Protein kinase domain

cl21453
Location:496 → 707

PKc_like; Protein Kinases, catalytic domain
NM_009445.3 → NP_033471.2 dual specificity protein kinase TTK isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1).

Source sequence(s)

AC124011

Consensus CDS

CCDS52874.1

UniProtKB/TrEMBL

Q6PD96

Related

ENSMUSP00000064839.8, ENSMUST00000070326.14

Conserved Domains (3) summary

cd14131
Location:496 → 764

PKc_Mps1; Catalytic domain of the Dual-specificity Mitotic checkpoint protein kinase, Monopolar spindle 1 (also called TTK)

sd00006
Location:105 → 134

TPR; TPR repeat [structural motif]

pfam00069
Location:498 → 764

Pkinase; Protein kinase domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

83716704..83754442

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006511060.2 → XP_006511123.1 dual specificity protein kinase TTK isoform X2

UniProtKB/TrEMBL

Q8BY97

Conserved Domains (2) summary

sd00006
Location:143 → 172

TPR; TPR repeat [structural motif]

cl21453
Location:535 → 722

PKc_like; Protein Kinases, catalytic domain
XM_006511058.1 → XP_006511121.1 dual specificity protein kinase TTK isoform X1

UniProtKB/TrEMBL

Q6PD96

Conserved Domains (2) summary

cd14131
Location:535 → 803

PKc_Mps1; Catalytic domain of the Dual-specificity Mitotic checkpoint protein kinase, Monopolar spindle 1 (also called TTK)

sd00006
Location:143 → 172

TPR; TPR repeat [structural motif]