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    Trpt1 tRNA phosphotransferase 1 [ Mus musculus (house mouse) ]

    Gene ID: 107328, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Trpt1provided by MGI
    Official Full Name
    tRNA phosphotransferase 1provided by MGI
    Primary source
    MGI:MGI:1333115
    See related
    Ensembl:ENSMUSG00000047656 AllianceGenome:MGI:1333115
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Tpt1; Tpt1h; Gm35838
    Summary
    Enables tRNA 2'-phosphotransferase activity. Acts upstream of or within regulation of protein kinase activity and tRNA splicing, via endonucleolytic cleavage and ligation. Is expressed in several structures, including gonad; intestine; liver; lung; and spleen. Orthologous to human TRPT1 (tRNA phosphotransferase 1). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in bladder adult (RPKM 9.3), adrenal adult (RPKM 8.6) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Trpt1 in Genome Data Viewer
    Location:
    19; 19 A
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (6970046..6977689)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (6996110..7000321)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E1971 Neighboring gene predicted gene, 54053 Neighboring gene vascular endothelial growth factor B Neighboring gene DnaJ heat shock protein family (Hsp40) member C4 Neighboring gene nudix hydrolase 22 Neighboring gene fermitin family member 3 Neighboring gene CapStarr-seq enhancer MGSCv37_chr19:7092729-7092882 Neighboring gene predicted gene, 54052 Neighboring gene STARR-seq mESC enhancer starr_45447 Neighboring gene stress-induced phosphoprotein 1 Neighboring gene STARR-seq mESC enhancer starr_45448 Neighboring gene CapStarr-seq enhancer MGSCv37_chr19:7140992-7141179 Neighboring gene mono-ADP ribosylhydrolase 1 Neighboring gene RIKEN cDNA 5730409K12 gene Neighboring gene fibronectin leucine rich transmembrane protein 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation. Harding HP, et al. RNA, 2008 Feb. PMID 18094117, Free PMC Article
    2. Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation. Yang X, et al. Nucleic Acids Res, 2023 Aug 11. PMID 37334830, Free PMC Article
    3. A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli. Spinelli SL, et al. Proc Natl Acad Sci U S A, 1998 Nov 24. PMID 9826666, Free PMC Article
    4. SAGE2Splice: unmapped SAGE tags reveal novel splice junctions. Kuo BY, et al. PLoS Comput Biol, 2006 Apr. PMID 16683015, Free PMC Article
    5. Spermatogonia-dependent expression of testicular genes in mice. Tanaka K, et al. Dev Biol, 2002 Jun 15. PMID 12051830

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation.
      Title: Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation.
    2. Inactivation of the Trpt1 gene, encoding the only known mammalian homolog of Tpt1p, eliminates all detectable 2'-phosphotransferase activity from cultured mouse cells but has no measurable effect on spliced Xbp-1 translation.
      Title: An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Clone Names

    • MGC41710

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables tRNA 2'-phosphotransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables tRNA 2'-phosphotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within regulation of protein kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA splicing, via endonucleolytic cleavage and ligation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within tRNA splicing, via endonucleolytic cleavage and ligation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cellular_component ND
    No biological Data available
    more info
    		  

    General protein information

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    Preferred Names
    tRNA 2'-phosphotransferase 1
    Names
    EST-MNCb3719
    mTPT1
    tRNA splicing 2' phosphotransferase 1 homolog
    NP_705825.2
    XP_006526640.1
    XP_006526641.1
    XP_006526642.1
    XP_036017277.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_153597.2 → NP_705825.2  tRNA 2'-phosphotransferase 1

      See identical proteins and their annotated locations for NP_705825.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and is protein-coding.
      Source sequence(s)
      AK038830, BC027575
      Consensus CDS
      CCDS50375.1
      UniProtKB/Swiss-Prot
      Q8K3A2
      Related
      ENSMUSP00000085555.6, ENSMUST00000088223.7
      Conserved Domains (1) summary
      pfam01885
      Location:21 → 198
      PTS_2-RNA; RNA 2'-phosphotransferase, Tpt1 / KptA family

    RNA

    1. NR_027351.1 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AK038830, BC027575
      Related
      ENSMUST00000237234.2

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      6970046..6977689
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036161384.1 → XP_036017277.1  tRNA 2'-phosphotransferase 1 isoform X1

      UniProtKB/Swiss-Prot
      Q8K3A2
      Conserved Domains (1) summary
      pfam01885
      Location:21 → 198
      PTS_2-RNA; RNA 2'-phosphotransferase, Tpt1 / KptA family

    2. XM_006526578.5 → XP_006526641.1  tRNA 2'-phosphotransferase 1 isoform X2

      Conserved Domains (1) summary
      pfam01885
      Location:26 → 180
      PTS_2-RNA; RNA 2'-phosphotransferase, Tpt1 / KptA family

    3. XM_006526579.3 → XP_006526642.1  tRNA 2'-phosphotransferase 1 isoform X3

      Conserved Domains (1) summary
      cl19470
      Location:48 → 83
      PTS_2-RNA; RNA 2'-phosphotransferase, Tpt1 / KptA family

    4. XM_006526577.5 → XP_006526640.1  tRNA 2'-phosphotransferase 1 isoform X1

      See identical proteins and their annotated locations for XP_006526640.1

      UniProtKB/Swiss-Prot
      Q8K3A2
      Conserved Domains (1) summary
      pfam01885
      Location:21 → 198
      PTS_2-RNA; RNA 2'-phosphotransferase, Tpt1 / KptA family
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8K3A2.2 GenPept UniProtKB/Swiss-Prot:Q8K3A2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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