Smurf2 SMAD specific E3 ubiquitin protein ligase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Smurf2provided by MGI
Official Full Name: SMAD specific E3 ubiquitin protein ligase 2provided by MGI
Primary source: MGI:MGI:1913563
See related: Ensembl:ENSMUSG00000018363 AllianceGenome:MGI:1913563
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 2810411E22Rik
Summary: Predicted to enable several functions, including SMAD binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Predicted to be involved in negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway; positive regulation of trophoblast cell migration; and proteasome-mediated ubiquitin-dependent protein catabolic process. Predicted to be located in nuclear speck. Predicted to be part of ubiquitin ligase complex. Predicted to be active in cytoplasm. Is expressed in several structures, including cardiovascular system; eye; genitourinary system; gut; and lung. Used to study diffuse large B-cell lymphoma. Orthologous to human SMURF2 (SMAD specific E3 ubiquitin protein ligase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in lung adult (RPKM 9.2), limb E14.5 (RPKM 7.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 E1; 11 70.03 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (106710887..106811544, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (106820061..106920747, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Flavin containing monooxygenase 2 regulates renal tubular cell fibrosis and paracrine secretion via SMURF2 in AKI-CKD transformation.
Title: Flavin containing monooxygenase 2 regulates renal tubular cell fibrosis and paracrine secretion via SMURF2 in AKI‑CKD transformation.
Smurf2 Regulates Inflammation and Collagen Processing in Cutaneous Wound Healing through Transforming Growth Factor-beta/Smad3 Signaling.
Title: Smurf2 Regulates Inflammation and Collagen Processing in Cutaneous Wound Healing through Transforming Growth Factor-β/Smad3 Signaling.
Inhibition of ChREBP ubiquitination via the ROS/Akt-dependent downregulation of Smurf2 contributes to lysophosphatidic acid-induced fibrosis in renal mesangial cells.
Title: Inhibition of ChREBP ubiquitination via the ROS/Akt-dependent downregulation of Smurf2 contributes to lysophosphatidic acid-induced fibrosis in renal mesangial cells.
MicroRNA-411-3p inhibits bleomycin-induced skin fibrosis by regulating transforming growth factor-beta/Smad ubiquitin regulatory factor-2 signalling.
Title: MicroRNA-411-3p inhibits bleomycin-induced skin fibrosis by regulating transforming growth factor-β/Smad ubiquitin regulatory factor-2 signalling.
Smurf2 exerts neuroprotective effects on cerebral ischemic injury.
Title: Smurf2 exerts neuroprotective effects on cerebral ischemic injury.
The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice.
Title: The Effects of WW2/WW3 Domains of Smurf2 Molecule on CD4+CD25+/CD4+ Proportion in Spleen of 4T1 Tumor Bearing BALB/c Mice.
Smurf2 negatively regulates MOR1 activity by reducing its stability in lung epithelial cells.
Title: The HECT ubiquitin E3 ligase Smurf2 degrades μ-opioid receptor 1 in the ubiquitin-proteasome system in lung epithelial cells.
although conventional Smurf2 double knockout mice are early embryonic lethal, Cre-mediated recombination in mouse embryonic fibroblasts results in Smurf2 knockout cells that show it has roles in regulating many different signaling pathways and in genomic stability, cell polarity, tissue homeostasis and carcinogenesis
Title: Generation of Smurf2 Conditional Knockout Mice.
Data suggest that Smad-specific E3 ubiquitin ligase 2 (SMURF2)-mediated SMAD3 protein (SMAD3) monoubiquitination interferes with the formation of a SMAD3-vitamin D receptor (VDR) complex.
Title: SMURF2 regulates bone homeostasis by disrupting SMAD3 interaction with vitamin D receptor in osteoblasts.
Results identified Smurf2 as an essential regulator of Topo IIalpha, providing novel insights into its control and into the suggested tumor-suppressor functions of Smurf2.
Title: Smurf2-Mediated Stabilization of DNA Topoisomerase IIα Controls Genomic Integrity.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (2)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MHAa88h11.seq (e-PCR)
- UniSTS:125443

30.MMHAP9FLB6.seq (e-PCR)
- UniSTS:123926

AI649275 (e-PCR)
- UniSTS:180353

AI558114 (e-PCR)
- UniSTS:164487

Smurf2 (e-PCR), detects polymorphism
- UniSTS:527558

34.MMHAP90FLD4.seq (e-PCR)
- UniSTS:124117

NoName (e-PCR)
- UniSTS:236763

RH140093 (e-PCR)
- UniSTS:222174

NoName (e-PCR)
- UniSTS:236766

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SMAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables SMAD binding	IEA Inferred from Electronic Annotation more info
enables SMAD binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transforming growth factor beta receptor binding	IEA Inferred from Electronic Annotation more info
enables transforming growth factor beta receptor binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	TAS Traceable Author Statement more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in negative regulation of BMP signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of trophoblast cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of trophoblast cell migration	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in membrane raft	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase SMURF2

Names: HECT-type E3 ubiquitin transferase SMURF2; SMAD ubiquitination regulatory factor 2

NP_001349823.1

EC 2.3.2.26

NP_079757.2

EC 2.3.2.26

XP_006533999.1

EC 2.3.2.26

XP_036012789.1

EC 2.3.2.26

XP_036012790.1

EC 2.3.2.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001362894.1 → NP_001349823.1 E3 ubiquitin-protein ligase SMURF2 isoform 2

Status: VALIDATED

Source sequence(s)

AL593847

Conserved Domains (2) summary

smart00456
Location:57 → 89

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:152 → 504

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
NM_025481.3 → NP_079757.2 E3 ubiquitin-protein ligase SMURF2 isoform 1

See identical proteins and their annotated locations for NP_079757.2

Status: VALIDATED

Source sequence(s)

AK012060, AK013082, AK036718, AL593847, BE986450, BU058366, BU614468

Consensus CDS

CCDS25564.1

UniProtKB/Swiss-Prot

A2A5Z6, A2A5Z7, Q5IRE6

Related

ENSMUSP00000090177.3, ENSMUST00000092517.9

Conserved Domains (5) summary

cd08382
Location:13 → 137

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:298 → 330

WW; Domain with 2 conserved Trp (W) residues

smart00119
Location:420 → 745

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd00078
Location:393 → 745

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00397
Location:159 → 188

WW; WW domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

106710887..106811544 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156896.1 → XP_036012789.1 E3 ubiquitin-protein ligase SMURF2 isoform X2

Conserved Domains (2) summary

cd08382
Location:13 → 137

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

COG5021
Location:159 → 625

HUL4; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]
XM_006533936.4 → XP_006533999.1 E3 ubiquitin-protein ligase SMURF2 isoform X1

UniProtKB/Swiss-Prot

A2A5Z6

Related

ENSMUSP00000099356.4, ENSMUST00000103067.10

Conserved Domains (2) summary

cd08382
Location:19 → 124

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

COG5021
Location:146 → 732

HUL4; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]
XM_036156897.1 → XP_036012790.1 E3 ubiquitin-protein ligase SMURF2 isoform X3

Conserved Domains (2) summary

smart00456
Location:57 → 89

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:152 → 504

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...