USP33 ubiquitin specific peptidase 33 [Homo sapiens (human)] - Gene

Summary

Official Symbol: USP33provided by HGNC
Official Full Name: ubiquitin specific peptidase 33provided by HGNC
Primary source: HGNC:HGNC:20059
See related: Ensembl:ENSG00000077254 MIM:615146; AllianceGenome:HGNC:20059
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: VDU1
Summary: This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]
Expression: Ubiquitous expression in fat (RPKM 19.0), brain (RPKM 15.9) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1p31.1

Exon count:: 27

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (77695987..77759852, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (77534817..77598662, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (78161672..78225537, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2.
Title: USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2.
USP33 enhances cell survival and stemness by deubiquitinating CTNNB1 in BXPC-3 and SW1990 cells.
Title: USP33 enhances cell survival and stemness by deubiquitinating CTNNB1 in BXPC-3 and SW1990 cells.
Downregulation of USP33 inhibits Slit/Robo signaling pathway and is associated with poor patient survival of glioma.
Title: Downregulation of USP33 inhibits Slit/Robo signaling pathway and is associated with poor patient survival of glioma.
USP33 deubiquitinates and stabilizes HIF-2alpha to promote hypoxia response in glioma stem cells.
Title: USP33 deubiquitinates and stabilizes HIF-2alpha to promote hypoxia response in glioma stem cells.
Ubiquitin specific peptidase 33 promotes cell proliferation and reduces apoptosis through regulation of the SP1/PI3K/AKT pathway in retinoblastoma.
Title: Ubiquitin specific peptidase 33 promotes cell proliferation and reduces apoptosis through regulation of the SP1/PI3K/AKT pathway in retinoblastoma.
Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Title: Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.
Title: USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.
SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia.
Title: SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia.
USP33 regulates c-Met expression by deubiquitinating SP1 to facilitate metastasis in hepatocellular carcinoma.
Title: USP33 regulates c-Met expression by deubiquitinating SP1 to facilitate metastasis in hepatocellular carcinoma.
Our study reveals the inhibitory function of Slit-Robo signalling in gastric cancer (GC) and uncovers a role of USP33 in suppressing cancer cell migration and epithelial-mesenchymal transition by enhancing Slit2-Robo1 signalling. USP33 represents a feasible choice as a prognostic biomarker for GC.
Title: Reduced USP33 expression in gastric cancer decreases inhibitory effects of Slit2-Robo1 signalling on cell migration and EMT.

Submit: New GeneRIF Correction See all GeneRIFs (28)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D1S3233 (e-PCR)
- UniSTS:154663

D8S2279 (e-PCR)
- UniSTS:473907

D1S3547 (e-PCR)
- UniSTS:21060

AFM270YC9 (e-PCR)
- UniSTS:6818

D11S2612 (e-PCR)
- UniSTS:70572

G34884 (e-PCR)
- UniSTS:29136

SHGC-75033 (e-PCR)
- UniSTS:476

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables cysteine-type deubiquitinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type deubiquitinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cysteine-type deubiquitinase activity	TAS Traceable Author Statement more info	PubMed
enables cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed
NOT enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in axon guidance	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in centrosome duplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein binding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of protein binding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein K48-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein deubiquitination	TAS Traceable Author Statement more info
involved_in protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
part_of VCB complex	TAS Traceable Author Statement more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	TAS Traceable Author Statement more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in focal adhesion	IDA Inferred from Direct Assay more info
located_in neuronal cell body	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ubiquitin carboxyl-terminal hydrolase 33

Names: VHL-interacting deubiquitinating enzyme 1; deubiquitinating enzyme 33; pVHL-interacting deubiquitinating enzyme 1; ubiquitin thioesterase 33; ubiquitin thiolesterase 33; ubiquitin-specific-processing protease 33

NP_001364359.1

EC 3.4.19.12

NP_001364360.1

EC 3.4.19.12

NP_001364361.1

EC 3.4.19.12

NP_001364362.1

EC 3.4.19.12

NP_001364363.1

EC 3.4.19.12

NP_001364364.1

EC 3.4.19.12

NP_001364365.1

EC 3.4.19.12

NP_001364366.1

EC 3.4.19.12

NP_001364367.1

EC 3.4.19.12

NP_001364368.1

EC 3.4.19.12

NP_055832.3

EC 3.4.19.12

NP_963918.1

EC 3.4.19.12

NP_963920.1

EC 3.4.19.12

XP_011539358.1

EC 3.4.19.12

XP_047305687.1

EC 3.4.19.12

XP_047305691.1

EC 3.4.19.12

XP_054191234.1

EC 3.4.19.12

XP_054191235.1

EC 3.4.19.12

XP_054191236.1

EC 3.4.19.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001377430.1 → NP_001364359.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 4

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (4) summary

smart00695
Location:832 → 916

DUSP; Domain in ubiquitin-specific proteases

COG5207
Location:39 → 285

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:185 → 704

UCH; Ubiquitin carboxyl-terminal hydrolase

cl12116
Location:726 → 795

DUSP; DUSP domain
NM_001377431.1 → NP_001364360.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 5

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (4) summary

smart00695
Location:801 → 885

DUSP; Domain in ubiquitin-specific proteases

COG5207
Location:8 → 254

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:154 → 673

UCH; Ubiquitin carboxyl-terminal hydrolase

cl12116
Location:695 → 764

DUSP; DUSP domain
NM_001377432.1 → NP_001364361.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 6

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

COG5207
Location:39 → 285

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:185 → 712

UCH; Ubiquitin carboxyl-terminal hydrolase

cl12116
Location:734 → 803

DUSP; DUSP domain
NM_001377433.1 → NP_001364362.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 7

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

smart00695
Location:741 → 825

DUSP; Domain in ubiquitin-specific proteases

COG5207
Location:39 → 285

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:185 → 671

UCH; Ubiquitin carboxyl-terminal hydrolase
NM_001377434.1 → NP_001364363.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 8

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

COG5207
Location:8 → 254

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:154 → 681

UCH; Ubiquitin carboxyl-terminal hydrolase

cl12116
Location:703 → 772

DUSP; DUSP domain
NM_001377435.1 → NP_001364364.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 9

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

smart00695
Location:710 → 794

DUSP; Domain in ubiquitin-specific proteases

COG5207
Location:8 → 254

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:154 → 640

UCH; Ubiquitin carboxyl-terminal hydrolase
NM_001377436.1 → NP_001364365.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 10

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

COG5207
Location:8 → 254

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:154 → 673

UCH; Ubiquitin carboxyl-terminal hydrolase

cl12116
Location:695 → 764

DUSP; DUSP domain
NM_001377437.1 → NP_001364366.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 11

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

smart00695
Location:702 → 786

DUSP; Domain in ubiquitin-specific proteases

COG5207
Location:8 → 254

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

pfam00443
Location:154 → 632

UCH; Ubiquitin carboxyl-terminal hydrolase
NM_001377438.1 → NP_001364367.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 12

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

smart00695
Location:661 → 745

DUSP; Domain in ubiquitin-specific proteases

cd02674
Location:294 → 534

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cl12116
Location:555 → 624

DUSP; DUSP domain
NM_001377439.1 → NP_001364368.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 12

Status: REVIEWED

Source sequence(s)

AC114487

Conserved Domains (3) summary

smart00695
Location:661 → 745

DUSP; Domain in ubiquitin-specific proteases

cd02674
Location:294 → 534

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cl12116
Location:555 → 624

DUSP; DUSP domain
NM_015017.5 → NP_055832.3 ubiquitin carboxyl-terminal hydrolase 33 isoform 1

See identical proteins and their annotated locations for NP_055832.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AF383172, AF383173, AI569971, BC016663

Consensus CDS

CCDS678.1

UniProtKB/Swiss-Prot

Q8TEY6, Q8TEY7, Q96AV6, Q9H9F0, Q9UPQ5

Related

ENSP00000359829.1, ENST00000370793.5

Conserved Domains (6) summary

smart00695
Location:840 → 924

DUSP; Domain in ubiquitin-specific proteases

cd02674
Location:473 → 713

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cl02553
Location:186 → 288

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam00443
Location:184 → 712

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam02148
Location:61 → 121

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein

cl12116
Location:734 → 803

DUSP; DUSP domain
NM_201624.3 → NP_963918.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 2

See identical proteins and their annotated locations for NP_963918.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region and uses a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AF383173, AI569971, BC016663

Consensus CDS

CCDS679.1

UniProtKB/Swiss-Prot

Q8TEY7

Related

ENSP00000359830.3, ENST00000370794.7

Conserved Domains (6) summary

smart00695
Location:809 → 893

DUSP; Domain in ubiquitin-specific proteases

cd02674
Location:442 → 682

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cl02553
Location:155 → 257

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam00443
Location:153 → 681

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam02148
Location:30 → 90

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein

cl12116
Location:703 → 772

DUSP; DUSP domain
NM_201626.3 → NP_963920.1 ubiquitin carboxyl-terminal hydrolase 33 isoform 3

See identical proteins and their annotated locations for NP_963920.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the coding region and differs in the 3' UTR compared to variant 1. The encoded isoform (3) has a shorter, distinct C-terminus compared to isoform 1.

Source sequence(s)

AC114487, AF383172, BC016663

Consensus CDS

CCDS680.1

UniProtKB/Swiss-Prot

Q8TEY7

Related

ENSP00000359828.3, ENST00000370792.7

Conserved Domains (3) summary

pfam00443
Location:185 → 704

UCH; Ubiquitin carboxyl-terminal hydrolase

cl02553
Location:39 → 285

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

cl12116
Location:726 → 795

DUSP; DUSP domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000001.11 Reference GRCh38.p14 Primary Assembly

Range

77695987..77759852 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047449735.1 → XP_047305691.1 ubiquitin carboxyl-terminal hydrolase 33 isoform X2
XM_047449731.1 → XP_047305687.1 ubiquitin carboxyl-terminal hydrolase 33 isoform X1
XM_011541056.3 → XP_011539358.1 ubiquitin carboxyl-terminal hydrolase 33 isoform X3

Conserved Domains (3) summary

cd02257
Location:461 → 595

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

cl02553
Location:184 → 289

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam02148
Location:61 → 121

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein

Alternate T2T-CHM13v2.0

Genomic

NC_060925.1 Alternate T2T-CHM13v2.0

Range

77534817..77598662 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054335260.1 → XP_054191235.1 ubiquitin carboxyl-terminal hydrolase 33 isoform X2
XM_054335259.1 → XP_054191234.1 ubiquitin carboxyl-terminal hydrolase 33 isoform X1
XM_054335261.1 → XP_054191236.1 ubiquitin carboxyl-terminal hydrolase 33 isoform X3