Dgat1 diacylglycerol O-acyltransferase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgat1provided by MGI
Official Full Name: diacylglycerol O-acyltransferase 1provided by MGI
Primary source: MGI:MGI:1333825
See related: Ensembl:ENSMUSG00000022555 AllianceGenome:MGI:1333825
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ARAT; Dgat; D15Ertd23e
Summary: Enables 2-acylglycerol O-acyltransferase activity; diacylglycerol O-acyltransferase activity; and retinol O-fatty-acyltransferase activity. Involved in several processes, including acylglycerol metabolic process; fatty acid homeostasis; and long-chain fatty-acyl-CoA metabolic process. Located in intracellular membrane-bounded organelle and membrane. Is active in endoplasmic reticulum membrane. Is expressed in several structures, including brain; genitourinary system; liver; skin; and yolk sac. Human ortholog(s) of this gene implicated in cardiomyopathy and congenital diarrhea 7 with exudative enteropathy. Orthologous to human DGAT1 (diacylglycerol O-acyltransferase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in duodenum adult (RPKM 359.0), small intestine adult (RPKM 242.2) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 D3; 15 35.99 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (76386215..76396167, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (76502012..76512021, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hypoxia-inducible lipid droplet-associated induces DGAT1 and promotes lipid storage in hepatocytes.
Title: Hypoxia-inducible lipid droplet-associated induces DGAT1 and promotes lipid storage in hepatocytes.
Hepatic synthesis of triacylglycerols containing medium-chain fatty acids is dominated by diacylglycerol acyltransferase 1 and efficiently inhibited by etomoxir.
Title: Hepatic synthesis of triacylglycerols containing medium-chain fatty acids is dominated by diacylglycerol acyltransferase 1 and efficiently inhibited by etomoxir.
Intestine-specific DGAT1 deficiency improves atherosclerosis in apolipoprotein E knockout mice by reducing systemic cholesterol burden.
Title: Intestine-specific DGAT1 deficiency improves atherosclerosis in apolipoprotein E knockout mice by reducing systemic cholesterol burden.
Lipid-Droplet Formation Drives Pathogenic Group 2 Innate Lymphoid Cells in Airway Inflammation.
Title: Lipid-Droplet Formation Drives Pathogenic Group 2 Innate Lymphoid Cells in Airway Inflammation.
Hepatic VLDL secretion: DGAT1 determines particle size but not particle number, which can be supported entirely by DGAT2
Title: Hepatic VLDL secretion: DGAT1 determines particle size but not particle number, which can be supported entirely by DGAT2.
Study demonstrated that Dgat1-/- mice have alterations in the process of autophagy-mediated cytoplasmic liquid droplets mobilization in enterocytes, likely due to the impairments of autophagic vesicles turnover and lysosome acidification during early stage of dietary fat absorption.
Title: DGAT1 deficiency disrupts lysosome function in enterocytes during dietary fat absorption.
Study shows that inactivation of DGAT1 or DGAT2 in adult mouse heart results in a moderate suppression of triglycerides (TG) synthesis and turnover. Partial inhibition of DGAT activity increases cardiac fatty acid oxidation without affecting PPARalpha signaling, myocardial energetics or contractile function. Thus, the two DGAT isoforms in the heart have partially redundant function.
Title: The Role of Diacylglycerol Acyltransferase (DGAT) 1 and 2 in Cardiac Metabolism and Function.
hese results demonstrate that DGAT1 suppresses retinol-dependent Treg formation and suggest its potential as a therapeutic target for autoimmune inflammation.
Title: DGAT1 inhibits retinol-dependent regulatory T cell formation and mediates autoimmune encephalomyelitis.
data reveal an important role for DGAT activity and TG synthesis generally in averting ER stress and lipotoxicity, with specifically DGAT1 performing this function during stimulated lipolysis in adipocytes.
Title: Triglyceride Synthesis by DGAT1 Protects Adipocytes from Lipid-Induced ER Stress during Lipolysis.
The endoplasmic reticulum-resident diacylglycerol acyltransferase 1 (DGAT1) selectively channels autophagy-liberated fatty acids into new, clustered lipid droplets that are in close proximity to mitochondria and are lipolytically degraded.
Title: DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy.

Submit: New GeneRIF Correction See all GeneRIFs (63)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dgat1 (e-PCR), detects polymorphism
- UniSTS:461913

RH125988 (e-PCR)
- UniSTS:162700

Dgat1 (e-PCR), detects polymorphism
- UniSTS:527311

AA859529 (e-PCR)
- UniSTS:243929

RH125195 (e-PCR)
- UniSTS:162017

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 2-acylglycerol O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables diacylglycerol O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables diacylglycerol O-acyltransferase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables diacylglycerol binding	ISO Inferred from Sequence Orthology more info
enables fatty acid binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables retinol O-fatty-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables retinol O-fatty-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in fatty acid homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fatty acid homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in ketone body metabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid storage	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid storage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-chain fatty-acyl-CoA metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in monoacylglycerol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in monoacylglycerol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fatty acid oxidation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of hepatic stellate cell activation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of triglyceride biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of eating behavior	ISO Inferred from Sequence Orthology more info
involved_in regulation of intestinal lipid absorption	ISO Inferred from Sequence Orthology more info
involved_in regulation of vitamin A metabolic process	ISO Inferred from Sequence Orthology more info
involved_in retinoid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in triglyceride biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in triglyceride biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in triglyceride biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within triglyceride biosynthetic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in triglyceride biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in very-low-density lipoprotein particle assembly	IEA Inferred from Electronic Annotation more info
involved_in very-low-density lipoprotein particle assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular membrane-bounded organelle	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: diacylglycerol O-acyltransferase 1

Names: acyl-CoA retinol O-fatty-acyltransferase; diglyceride acyltransferase

NP_034176.1

EC 2.3.1.20

EC 2.3.1.76

XP_006520468.1

EC 2.3.1.20

EC 2.3.1.76

XP_017171915.1

EC 2.3.1.20

EC 2.3.1.76

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010046.4 → NP_034176.1 diacylglycerol O-acyltransferase 1

See identical proteins and their annotated locations for NP_034176.1

Status: VALIDATED

Source sequence(s)

AC157566

Consensus CDS

CCDS27573.1

UniProtKB/Swiss-Prot

Q9D7Q5, Q9Z2A7

UniProtKB/TrEMBL

Q54AA6

Related

ENSMUSP00000023214.5, ENSMUST00000023214.11

Conserved Domains (1) summary

cl00738
Location:79 → 483

MBOAT; MBOAT, membrane-bound O-acyltransferase family

RNA

NR_177195.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC157566

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

76386215..76396167 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520405.4 → XP_006520468.1 diacylglycerol O-acyltransferase 1 isoform X1

Conserved Domains (1) summary

cl00738
Location:8 → 418

MBOAT; membrane-bound O-acyltransferase family
XM_017316426.1 → XP_017171915.1 diacylglycerol O-acyltransferase 1 isoform X2

Conserved Domains (1) summary

cl00738
Location:2 → 312

MBOAT; membrane-bound O-acyltransferase family