RQC2 Rqc2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RQC2
Official Full Name: Rqc2p
Primary source: SGD:S000005930
Locus tag: YPL009C
See related: AllianceGenome:SGD:S000005930; FungiDB:YPL009C; VEuPathDB:YPL009C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TAE2
Summary: Enables alpha-aminoacyl-tRNA binding activity; ribosomal large subunit binding activity; and tRNA binding activity. Involved in CAT tailing and peptide biosynthetic process. Located in cytoplasmic stress granule. Part of RQC complex. Is active in cytosolic ribosome. Used to study neuromuscular disease. Human ortholog(s) of this gene implicated in neuromuscular disease. Orthologous to human NEMF (nuclear export mediator factor). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RQC2 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (535820..538936, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Alternatively, Pol III perturbation may alter the cellular pool of tRNAs and, thereby, the tRNAs that Rqc2 recruits to synthesize CAT tails. Indeed, disruption of Pol III altered the mobility of soluble CAT tails by SDS-PAGE, indicative of a change in composition
Title: Aggregation of CAT tails blocks their degradation and causes proteotoxicity in S. cerevisiae.
The authors determined that the Rqc2p-mediated Carboxy-terminal Alanine and Threonine (CAT) tail elongation is mechanistically distinct from canonical translation, that Ltn1p-mediated ubiquitination depends on the poorly characterized Ribosome-associated Quality control Complex (RQC) component Rqc1p, and that the process of CAT tailing enables robust ubiquitination of the nascent polypeptide.
Title: In vitro analysis of RQC activities provides insights into the mechanism and function of CAT tailing.
Rqc2 modifies stalled nascent polypeptide chains with a carboxy-terminal, Ala- and Thr-containing extension-the 'CAT tail'.
Title: The Rqc2/Tae2 subunit of the ribosome-associated quality control (RQC) complex marks ribosome-stalled nascent polypeptide chains for aggregation.
Data show that Saccharomyces cerevisiae proteins Rqc1, Ltn1, and Cdc48 prevent the aggregation of aberrant proteins that can only appear after C-terminal alanine-threonine tail (CAT-tail) addition by Rqc2.
Title: Rqc1 and Ltn1 Prevent C-terminal Alanine-Threonine Tail (CAT-tail)-induced Protein Aggregation by Efficient Recruitment of Cdc48 on Stalled 60S Subunits.
protein aggregation is dependent on a C-terminal alanine/threonine tail that is added to stalled polypeptides by the RQC component, Rqc2p
Title: Failure of RQC machinery causes protein aggregation and proteotoxic stress.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables alpha-aminoacyl-tRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	HDA more info	PubMed
enables ribosomal large subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables ribosomal large subunit binding	IDA Inferred from Direct Assay more info	PubMed
enables tRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables tRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in CAT tailing	IDA Inferred from Direct Assay more info	PubMed
involved_in peptide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rescue of stalled ribosome	IBA Inferred from Biological aspect of Ancestor more info
involved_in rescue of stalled ribosome	IDA Inferred from Direct Assay more info	PubMed
involved_in rescue of stalled ribosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome-associated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ribosome-associated ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ribosome-associated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of RQC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RQC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RQC complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosolic ribosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rqc2p

NP_015316.1

Component of the Ribosome Quality Control (RQC) complex; RQC is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs noncanonical elongation of nascent chains independently of mRNA or 40S subunits in a process called CAT (carboxyl-terminal alanine and threonine) tailing, promoting LTN1-mediated ubiquitination; monitors translation stress and signals this to Hsf1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

535820..538936 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183823.1 → NP_015316.1 TPA: Rqc2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015316.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W403, Q12532

UniProtKB/TrEMBL

N1P1W0

Conserved Domains (2) summary

COG1293
Location:1 → 699

YloA; Predicted component of the ribosome quality control (RQC) complex, YloA/Tae2 family, contains fibronectin-binding (FbpA) and DUF814 domains [Translation, ribosomal structure and biogenesis]

pfam11923
Location:915 → 1017

DUF3441; Domain of unknown function (DUF3441)