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    SPA2 Spa2p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850639, updated on 9-Dec-2024

    Summary

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    Official Symbol
    SPA2
    Official Full Name
    Spa2p
    Primary source
    SGD:S000003944
    Locus tag
    YLL021W
    See related
    AllianceGenome:SGD:S000003944; FungiDB:YLL021W; VEuPathDB:YLL021W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    FUS6; PEA1; PPF1
    Summary
    Enables MAP-kinase scaffold activity. Involved in several processes, including bipolar cellular bud site selection; budding cell apical bud growth; and pseudohyphal growth. Located in several cellular components, including cellular bud tip; incipient cellular bud site; and mating projection tip. Part of polarisome. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See SPA2 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (100947..105347)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene nucleoporin POM33 Neighboring gene Hif1p Neighboring gene serine/threonine protein kinase KNS1 Neighboring gene Cox19p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating. Gehrung S, et al. J Cell Biol, 1990 Oct. PMID 2211820, Free PMC Article
    2. The SPA2 protein of yeast localizes to sites of cell growth. Snyder M. J Cell Biol, 1989 Apr. PMID 2647769, Free PMC Article
    3. The polarisome component SpaA localises to hyphal tips of Aspergillus niger and is important for polar growth. Meyer V, et al. Fungal Genet Biol, 2008 Feb. PMID 17826199
    4. Afr1p has a role in regulating the localization of Mpk1p at the shmoo tip in Saccharomyces cerevisiae. Changwei Z, et al. FEBS Lett, 2007 Jun 12. PMID 17509572
    5. A small conserved domain in the yeast Spa2p is necessary and sufficient for its polarized localization. Arkowitz RA, et al. J Cell Biol, 1997 Jul 14. PMID 9214378, Free PMC Article

    See all (144) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Spa2 remodels ADP-actin via molecular condensation under glucose starvation.
      Title: Spa2 remodels ADP-actin via molecular condensation under glucose starvation.
    2. A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.
      Title: A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.
    3. The molecular dynamics and reversibility of Aip5 condensates are regulated by scaffolding protein Spa2 via liquid-liquid phase separation both in vitro and in vivo. In the absence of Spa2, Aip5 condensates hamper cell growth and actin cable structures under stress treatment.
      Title: Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.
    4. data indicate that Spa2 is recruited to the cleavage site to co-operate with the secretory vesicle system and particular actomyosin ring components to promote the incorporation of Chs2 into the so-called 'ingression progression complexes' during cytokinesis in budding yeast
      Title: Cell polarity protein Spa2 coordinates Chs2 incorporation at the division site in budding yeast.
    5. the pea2-M409L mutation results in decreased invasive filamentation and elongation, diminished activity of a Kss1p MAPK pathway reporter, decreased unipolar budding, and diminished binding of the polarisome protein Spa2p.
      Title: Pooled segregant sequencing reveals genetic determinants of yeast pseudohyphal growth.
    6. Therefore, we suggest that Mid1 function is influenced by Spa2 function through polarized morphogenesis.
      Title: Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae.
    7. findings show Cdc42 serves an essential function in the establishment of bud site by catalyzing translocation of bud-site components such as Spa2 to the cell cortex; role of Cdc42 in the cortical localization of Spa2 appears to be independent of Cdc24
      Title: Cdc42-dependent localization of polarisome component Spa2 to the incipient bud site is independent of the GDP/GTP exchange factor Cdc24.
    8. Spa2p is involved in cytokinesis and cell wall morphogenesis.
      Title: Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables MAP-kinase scaffold activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables MAP-kinase scaffold activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables MAP-kinase scaffold activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in MAPK cascade IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in bipolar cellular bud site selection IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in bipolar cellular bud site selection IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in budding cell apical bud growth IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in budding cell apical bud growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular hyperosmotic response IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in cellular hyperosmotic response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to acidic pH IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in establishment of cell polarity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in invasive filamentous growth IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in invasive filamentous growth IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in pseudohyphal growth IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in pseudohyphal growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of actin cytoskeleton organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of actin cytoskeleton organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell shape IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of protein localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in actomyosin contractile ring IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell cortex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cell cortex of growing cell tip IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell tip IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cellular bud neck IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cellular bud neck IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cellular bud tip IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cellular bud tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cellular bud tip IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    is_active_in incipient cellular bud site IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in incipient cellular bud site IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mating projection tip IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mating projection tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of polarisome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Spa2p
    NP_013079.1
    • Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      100947..105347
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181841.1 → NP_013079.1  TPA: Spa2p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013079.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXY3, P23201
      UniProtKB/TrEMBL
      N1P021
      Conserved Domains (2) summary
      pfam08518
      Location:94 → 122
      GIT_SHD; Spa2 homology domain (SHD) of GIT
      pfam15140
      Location:876 → 1042
      DUF4573; Domain of unknown function (DUF4573)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P23201.1

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