DYN1 dynein heavy chain [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DYN1
Official Full Name: dynein heavy chain
Primary source: SGD:S000001762
Locus tag: YKR054C
See related: AllianceGenome:SGD:S000001762; FungiDB:YKR054C; VEuPathDB:YKR054C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DHC1; PAC6
Summary: Enables ATP binding activity and minus-end-directed microtubule motor activity. Involved in establishment of mitotic spindle orientation; mitotic sister chromatid segregation; and nuclear migration along microtubule. Located in astral microtubule; cell cortex; and spindle pole body. Part of cytoplasmic dynein complex. Used to study Charcot-Marie-Tooth disease axonal type 2O; congenital muscular dystrophy; lissencephaly; polymicrogyria; and spinal muscular atrophy with lower extremity predominant 1. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2O; autosomal dominant intellectual developmental disorder 13; lissencephaly; and spinal muscular atrophy with lower extremity predominant 1. Orthologous to human DYNC1H1 (dynein cytoplasmic 1 heavy chain 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DYN1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (535647..547925, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.
Title: Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.
Dynein's anisotropic response to directional tension is mediated by sliding of the coiled-coils of the stalk, and that coordinated conformational changes of dynein's linker and buttress control this process.
Title: Molecular mechanism of cytoplasmic dynein tension sensing.
using single-molecule assays, elongation or shortening of the coiled-coil stalk that connects the motor to the microtubule controls the helical directionality of dynein around microtubules; changing the length and angle of the stalk successfully reversed the motility towards the plus end of the microtubule
Title: Directionality of dynein is controlled by the angle and length of its stalk.
Data suggest a model in which simultaneous interactions between the microtubule and dynein enables myosin 4 (She1) to directly affect dynein motility.
Title: She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain.
Interplay among dynein, dynactin, and the stability of the microtubule substrate creates a mechanism that regulates accurate spindle positioning.
Title: Dynein is regulated by the stability of its microtubule track.
Lis1 (Pac1) regulates dynein by sterically blocking its mechanochemical cycle.
Title: Lis1 regulates dynein by sterically blocking its mechanochemical cycle.
DYN1 is engaged in bidirectional helical motility around microtubules in the process of cargo transport.
Title: Bidirectional helical motility of cytoplasmic dynein around microtubules.
Study reports X-ray and electron microscopy structures of yeast dynein, DYN1 bound to different ATP analogs, which collectively provide insight into the roles of dynein's two major ATPase sites, AAA1 and AAA3, in the conformational change mechanism.
Title: Allosteric communication in the dynein motor domain.
Results indicate that inhibition of dynein activity by She1 is important to prevent excessive detachment of cytoplasmic microtubules, particularly in G1 cells.
Title: Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body.
The authors find that dynein has a highly variable stepping pattern that is distinct from all other processive cytoskeletal motors, which use 'hand-over-hand' mechanisms.
Title: Dynein achieves processive motion using both stochastic and coordinated stepping.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables dynein intermediate chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables dynein intermediate chain binding	IEA Inferred from Electronic Annotation more info
enables dynein light intermediate chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables dynein light intermediate chain binding	IEA Inferred from Electronic Annotation more info
enables minus-end-directed microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables minus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables minus-end-directed microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables minus-end-directed microtubule motor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cytoplasmic microtubule organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of mitotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in karyogamy	IEA Inferred from Electronic Annotation more info
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear migration along microtubule	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nuclear migration along microtubule	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in astral microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of cytoplasmic dynein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cytoplasmic dynein complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasmic microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
part_of dynein complex	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dynein heavy chain

NP_012980.1

Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

535647..547925 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179844.1 → NP_012980.1 TPA: dynein heavy chain [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012980.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXB5, P36022

UniProtKB/TrEMBL

C7GNL1, N1P0I4

Conserved Domains (2) summary

COG5245
Location:774 → 4083

DYN1; Dynein, heavy chain [Cytoskeleton]

pfam08385
Location:219 → 732

DHC_N1; Dynein heavy chain, N-terminal region 1