PRK1 serine/threonine protein kinase PRK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PRK1
Official Full Name: serine/threonine protein kinase PRK1
Primary source: SGD:S000001357
Locus tag: YIL095W
See related: AllianceGenome:SGD:S000001357; FungiDB:YIL095W; VEuPathDB:YIL095W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PAK1
Summary: Enables protein serine/threonine kinase activity. Involved in actin cortical patch assembly and negative regulation of cellular component organization. Located in actin cortical patch. Orthologous to human AAK1 (AP2 associated kinase 1) and BMP2K (BMP2 inducible kinase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PRK1 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (183937..186369)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conserved Ark1-related kinases function in a TORC2 signaling network.
Title: Conserved Ark1-related kinases function in a TORC2 signaling network.
autophosphorylation of Prk1p may represent a feedback mechanism possibly involved in fine-tuning the pace of progression during actin-coupled endocytosis
Title: Negative regulation of the actin-regulating kinase Prk1p by patch localization-induced autophosphorylation.
These findings reveal that Arp2p also mediates what appears to be an auto-regulatory mechanism possibly adapted for efficient coordination of actin assembly and disassembly during endocytosis.
Title: A novel function of Arp2p in mediating Prk1p-specific regulation of actin and endocytosis in yeast.
Overexpressed Prk1 conferred Adriamycin resistance in yeast cells.
Title: Endocytic Ark/Prk kinases play a critical role in adriamycin resistance in both yeast and mammalian cells.
Data show that Sjl2p is specifically associated with cortical actin patches which aggregate upon loss of the actin-regulating kinases Ark1p and Prk1p.
Title: Sjl2p is specifically involved in early steps of endocytosis intimately linked to actin dynamics via the Ark1p/Prk1p kinases.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cortical patch assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cortical patch assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of actin cortical patch assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of clathrin-dependent endocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of clathrin-dependent endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in mating projection tip	HDA more info	PubMed

General protein information

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Preferred Names: serine/threonine protein kinase PRK1

NP_012171.1

EC 2.7.11.1

Ser/Thr protein kinase; regulates the organization and function of the actin cytoskeleton; inhibits clathrin-mediated endocytosis; phosphorylates the Pan1p and Sla1p subunits of the Pan1p-Sla1p-End3p complex, resulting in inhibition of complex formation; inhibits Scd5p through phosphorylation; phosphorylates Pan1p-interacting proteins, Ent1/2 and Yap1801/2; negatively regulated through autophosphorylation; functional overlap with ARK1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

183937..186369

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179443.1 → NP_012171.1 TPA: serine/threonine protein kinase PRK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012171.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVJ2, P40494, Q02553

UniProtKB/TrEMBL

A6ZVI2, B3LTU2, C7GKM2, C8ZAF5, N1P9B6

Conserved Domains (1) summary

cd14037
Location:18 → 300

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases