MSS116 ATP-dependent RNA helicase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSS116
Official Full Name: ATP-dependent RNA helicase
Primary source: SGD:S000002602
Locus tag: YDR194C
See related: AllianceGenome:SGD:S000002602; FungiDB:YDR194C; VEuPathDB:YDR194C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA helicase activity and RNA strand annealing activity. Involved in RNA folding and RNA metabolic process. Located in mitochondrial matrix. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MSS116 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (845952..847946, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mss116 plays roles in the assembly of the mitochondrial translational machinery as well as in the translation process itself.
Title: The DEAD-box helicase Mss116 plays distinct roles in mitochondrial ribogenesis and mRNA-specific translation.
Crystal structures of MSS116 reveal that core stability is modulated by family-specific interactions that favor certain RNA or DNA substrates.
Title: Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
overexpression of RPO41 or MSS116 increases cell survival from colonies that were exposed to low temperature, suggesting a role for Mss116p in enhancing the efficiency of mitochondrial transcription under stress conditions.
Title: Yeast DEAD box protein Mss116p is a transcription elongation factor that modulates the activity of mitochondrial RNA polymerase.
The two strands of double strands RNA are recognized asymmetrically by the RNA-structure modulating Mss116p.
Title: Exploring the molecular basis of dsRNA recognition by Mss116p using molecular dynamics simulations and free-energy calculations.
Mss116 stabilizes an early, obligate folding intermediate within intron domain 1.
Title: The brace for a growing scaffold: Mss116 protein promotes RNA folding by stabilizing an early assembly intermediate.
results provide a comprehensive structural model for how DEAD-box proteins (like Mss116) recognize and unwind RNA duplexes
Title: Structural basis for RNA-duplex recognition and unwinding by the DEAD-box helicase Mss116p.
a critical activity in the Mss116p-mediated folding of the aI5-gamma intron is ATP-dependent RNA-unwinding activity.
Title: ATP-dependent roles of the DEAD-box protein Mss116p in group II intron splicing in vitro and in vivo.
We measured the rate and equilibrium constants underlying Mss116 ATP utilization and nucleotide-linked RNA binding. RNA accelerates the Mss116 steady-state ATPase approximately 7- fold by promoting rate-limiting ATP hydrolysis.
Title: Mechanism of Mss116 ATPase reveals functional diversity of DEAD-Box proteins.
The Mss116 protein stimulates group II intron self-splicing in vitro through two distinct mechanisms that can be differentiated by varying the length of precursor exons and examining the function of protein mutants.
Title: Dual roles for the Mss116 cofactor during splicing of the ai5γ group II intron.
Together, these observations suggest that the efficiency by which Mss116p catalyzes the hydrolysis of ATP is critical for all of its splicing functions in vivo.
Title: Structure-guided mutational analysis of a yeast DEAD-box protein involved in mitochondrial RNA splicing.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables G-quadruplex RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables RNA strand annealing activity	IDA Inferred from Direct Assay more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Group I intron splicing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Group II intron splicing	IDA Inferred from Direct Assay more info	PubMed
involved_in Group II intron splicing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial RNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in transcription elongation by mitochondrial RNA polymerase	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription elongation by mitochondrial RNA polymerase	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ATP-dependent RNA helicase

NP_010480.1

EC 3.6.4.13

Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate; binds G-quadruplex (G4) nucleic acids and destabilizes G4RNA structures

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

845952..847946 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180502.1 → NP_010480.1 TPA: ATP-dependent RNA helicase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010480.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSH6, P15424

UniProtKB/TrEMBL

B3LG90, C7GN04, N1PAF2

Conserved Domains (1) summary

COG0513
Location:76 → 579

SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]