Hsf4 heat shock transcription factor 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hsf4provided by MGI
Official Full Name: heat shock transcription factor 4provided by MGI
Primary source: MGI:MGI:1347058
See related: Ensembl:ENSMUSG00000033249 AllianceGenome:MGI:1347058
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: HSF 4; HSTF4; ldis1; mHSF4; HSTF 4
Summary: Predicted to enable several functions, including RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; identical protein binding activity; and protein phosphatase binding activity. Predicted to be involved in lens fiber cell differentiation and negative regulation of transcription by RNA polymerase II. Predicted to be located in chromatin and nuclear speck. Predicted to be active in nucleus. Is expressed in several structures, including brain; lens; respiratory system; submandibular gland; and urinary system. Used to study cataract 5 multiple types. Human ortholog(s) of this gene implicated in cataract and cataract 5 multiple types. Orthologous to human HSF4 (heat shock transcription factor 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in lung adult (RPKM 9.1), cortex adult (RPKM 6.1) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 D3; 8 53.04 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (105996392..106002477)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (105269414..105275845)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation.
Title: HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation.
Deficiency of heat shock factor 4 promotes lens epithelial cell senescence through upregulating p21(cip1) expression.
Title: Deficiency of heat shock factor 4 promotes lens epithelial cell senescence through upregulating p21(cip1) expression.
The aging mouse lens transcriptome.
Title: The aging mouse lens transcriptome.
During lens development, phosphorylation of HSF4/S299 is downregulated in P3 lens and upregulated in P7 and P14 lens. However, in 2 months old lens, both phosphorylation of HSF4/S299 and total HSF4 protein are decreased.
Title: Downregulation of heat shock factor 4 transcription activity via MAPKinase phosphorylation at Serine 299.
a novel molecular mechanism of HSF4 in regulating lens epithelial cell homeostasis
Title: Heat shock factor 4 regulates lens epithelial cell homeostasis by working with lysosome and anti-apoptosis pathways.
BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination.
Title: BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination.
The deletion of Hsf4 affects the expression of many genes, such as Ubc, Ptgs2, Egr1 and Fos. These genes may be involved in the development of cataract.
Title: Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens.
A novel regulatory mechanism between Hsf1 and Hsf4b in modulating lens epithelial cell homeostasis.
Title: Hsf4 counteracts Hsf1 transcription activities and increases lens epithelial cell survival in vitro.
Mutations in the DNA binding domain (DBD) of the heat shock transcription factor 4 (HSF4) are known to be associated with early childhood autosomal dominant lamellar cataract.
Title: Expression of the HSF4 DNA binding domain-EGFP hybrid gene recreates early childhood lamellar cataract in transgenic mice.
This comparative analysis with CRYAB and HSP70 demonstrates that differential heat shock response is controlled by cell-type-dependent access of HSF1 and HSF4 to specific promoters, independent of the promoter architecture.
Title: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Spontaneous (1) 1 citation
Targeted (5) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:490768

NoName (e-PCR)
- UniSTS:495050

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein phosphatase binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in lens fiber cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in chromatin	IEA Inferred from Electronic Annotation more info
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: heat shock factor protein 4

Names: DNA binding protein; binds heat shock DNA elements; heat shock factor 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001256042.1 → NP_001242971.1 heat shock factor protein 4 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC124584

Consensus CDS

CCDS57639.1

UniProtKB/Swiss-Prot

Q9R0L1, Q9R0L2

UniProtKB/TrEMBL

B2RQY2, Q9JIZ7

Related

ENSMUSP00000048904.3, ENSMUST00000036127.9

Conserved Domains (2) summary

smart00415
Location:16 → 120

HSF; heat shock factor

pfam15035
Location:110 → 175

Rootletin; Ciliary rootlet component, centrosome cohesion
NM_001256044.1 → NP_001242973.1 heat shock factor protein 4 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an exon in the coding region, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1.

Source sequence(s)

AC124584, AK148056

Consensus CDS

CCDS57640.1

UniProtKB/TrEMBL

Q3UG93, Q9JIZ7

Related

ENSMUSP00000126278.3, ENSMUST00000163734.9

Conserved Domains (2) summary

pfam15035
Location:50 → 115

Rootletin; Ciliary rootlet component, centrosome cohesion

cl12113
Location:18 → 60

HSF_DNA-bind; HSF-type DNA-binding
NM_011939.3 → NP_036069.1 heat shock factor protein 4 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses two alternate in-frame splice sites in that result in a frameshift in the central coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct internal segment, compared to isoform 1.

Source sequence(s)

AC124584

Consensus CDS

CCDS57638.1

UniProtKB/TrEMBL

Q9JIZ7, Q9JIZ8

Related

ENSMUSP00000134213.2, ENSMUST00000173859.2

Conserved Domains (2) summary

smart00415
Location:16 → 120

HSF; heat shock factor

pfam15035
Location:110 → 175

Rootletin; Ciliary rootlet component, centrosome cohesion

RNA

NR_045689.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses an alternate splice site, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC124584

Related

ENSMUST00000173640.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

105996392..106002477

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006531041.4 → XP_006531104.1 heat shock factor protein 4 isoform X4
XM_017312837.3 → XP_017168326.1 heat shock factor protein 4 isoform X1

UniProtKB/TrEMBL

Q9JIZ7

Conserved Domains (1) summary

smart00415
Location:16 → 120

HSF; heat shock factor
XM_006531038.5 → XP_006531101.1 heat shock factor protein 4 isoform X2

UniProtKB/TrEMBL

Q9JIZ7

Conserved Domains (1) summary

smart00415
Location:16 → 120

HSF; heat shock factor
XM_006531039.3 → XP_006531102.1 heat shock factor protein 4 isoform X3

UniProtKB/TrEMBL

Q9JIZ7

Conserved Domains (2) summary

smart00415
Location:1 → 53

HSF; heat shock factor

pfam06546
Location:192 → 417

Vert_HS_TF; Vertebrate heat shock transcription factor