Gbp4 guanylate binding protein 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gbp4provided by MGI
Official Full Name: guanylate binding protein 4provided by MGI
Primary source: MGI:MGI:97072
See related: Ensembl:ENSMUSG00000079363 AllianceGenome:MGI:97072
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mpa2; GBP-4; Mag-2; Mpa-2
Summary: Enables ubiquitin-protein transferase inhibitor activity. Involved in negative regulation of interferon-alpha production. Acts upstream of or within several processes, including cellular response to type II interferon; negative regulation of macromolecule metabolic process; and negative regulation of protein import into nucleus. Predicted to be located in Golgi membrane; nucleus; and perinuclear region of cytoplasm. Predicted to be active in cytoplasmic vesicle. Orthologous to human GBP6 (guanylate binding protein family member 6). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in thymus adult (RPKM 15.2), bladder adult (RPKM 6.1) and 10 other tissues See more
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Genomic context

Location:: 5 E5; 5 50.68 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (105263633..105287452, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (105115767..105139586, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GBP4 is a negative regulator of virus-triggered type I interferon (IFN-I) production, interacting as a novel protein targeting interferon regulatory factor (IRF)-7 and inhibiting its function.
Title: Guanylate binding protein 4 negatively regulates virus-induced type I IFN and antiviral response by targeting IFN regulatory factor 7.
Two splice variants of mGBP4 could be detected. One variant led to a premature stop codon after 312 bp. The second variant resulted in a transcript with a disrupted G2 domain.
Title: In silico and in vitro characterization of mGBP4 splice variants.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin-protein transferase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to type II interferon	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cellular response to type II interferon	IDA Inferred from Direct Assay more info	PubMed
involved_in defense response to Gram-positive bacterium	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to protozoan	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of interferon-alpha production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of interferon-alpha production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein import into nucleus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of protein ubiquitination	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasmic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: guanylate-binding protein 4

Names: GTP-binding protein 4; guanine nucleotide-binding protein 4; guanylate nucleotide binding protein 4; guanylate-binding protein 4 homolog; macrophage activation 2

NP_001242934.1

EC 3.6.5.-

NP_001346008.1

EC 3.6.5.-

NP_001346009.1

EC 3.6.5.-

NP_001346010.1

EC 3.6.5.-

NP_001346011.1

EC 3.6.5.-

NP_032646.2

EC 3.6.5.-

XP_006534867.1

EC 3.6.5.-

XP_006534868.1

EC 3.6.5.-

XP_011247713.1

EC 3.6.5.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001256005.1 → NP_001242934.1 guanylate-binding protein 4 isoform 1

See identical proteins and their annotated locations for NP_001242934.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) lacks an alternate exon in the 5' end compared to variant 1. The resulting isoform (1) is shorter at the N-terminus compared to isoform 3.

Source sequence(s)

EF494423

Consensus CDS

CCDS57368.1

UniProtKB/Swiss-Prot

A0A0G2JDT5, A0A0G2JEJ5, A0A0G2JGT4, A4UUI3, B9EJ74, Q61594

UniProtKB/TrEMBL

Q5DTF3

Related

ENSMUSP00000098522.4, ENSMUST00000100962.8

Conserved Domains (2) summary

pfam02841
Location:289 → 582

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:16 → 287

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001359079.1 → NP_001346008.1 guanylate-binding protein 4 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3).

Source sequence(s)

AC123697, AC144914

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:302 → 595

GBP_C; Guanylate-binding protein, C-terminal domain

cl21455
Location:29 → 300

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001359080.1 → NP_001346009.1 guanylate-binding protein 4 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3. Variants 2, 4, 5, and 6 all encode the same isoform (2).

Source sequence(s)

AC123697, AC144914

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:153 → 446

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:1 → 151

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001359081.1 → NP_001346010.1 guanylate-binding protein 4 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3. Variants 2, 4, 5, and 6 all encode the same isoform (2).

Source sequence(s)

AC123697, AC144914

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:153 → 446

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:1 → 151

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001359082.1 → NP_001346011.1 guanylate-binding protein 4 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3. Variants 2, 4, 5, and 6 all encode the same isoform (2).

Source sequence(s)

AC123697, AC144914

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:153 → 446

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:1 → 151

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_008620.4 → NP_032646.2 guanylate-binding protein 4 isoform 2

See identical proteins and their annotated locations for NP_032646.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3. Variants 2, 4, 5, and 6 all encode the same isoform (2).

Source sequence(s)

EF494424

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:153 → 446

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:1 → 151

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

105263633..105287452 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006534805.4 → XP_006534868.1 guanylate-binding protein 4 isoform X1

See identical proteins and their annotated locations for XP_006534868.1

UniProtKB/Swiss-Prot

A0A0G2JDT5, A0A0G2JEJ5, A0A0G2JGT4, A4UUI3, B9EJ74, Q61594

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:289 → 582

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:16 → 287

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
XM_006534804.4 → XP_006534867.1 guanylate-binding protein 4 isoform X1

See identical proteins and their annotated locations for XP_006534867.1

UniProtKB/Swiss-Prot

A0A0G2JDT5, A0A0G2JEJ5, A0A0G2JGT4, A4UUI3, B9EJ74, Q61594

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:289 → 582

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:16 → 287

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
XM_011249411.4 → XP_011247713.1 guanylate-binding protein 4 isoform X1

See identical proteins and their annotated locations for XP_011247713.1

UniProtKB/Swiss-Prot

A0A0G2JDT5, A0A0G2JEJ5, A0A0G2JGT4, A4UUI3, B9EJ74, Q61594

UniProtKB/TrEMBL

Q5DTF3

Conserved Domains (2) summary

pfam02841
Location:289 → 582

GBP_C; Guanylate-binding protein, C-terminal domain

cl38936
Location:16 → 287

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases