Siah1a siah E3 ubiquitin protein ligase 1A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Siah1aprovided by MGI
Official Full Name: siah E3 ubiquitin protein ligase 1Aprovided by MGI
Primary source: MGI:MGI:108064
See related: Ensembl:ENSMUSG00000036840 AllianceGenome:MGI:108064
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Sinh1a
Summary: Enables several functions, including disordered domain specific binding activity; protein homodimerization activity; and zinc ion binding activity. Involved in canonical Wnt signaling pathway and proteasome-mediated ubiquitin-dependent protein catabolic process. Acts upstream of or within several processes, including male meiosis I; regulation of growth; and spermatogenesis. Part of SCF ubiquitin ligase complex. Is expressed in several structures, including alimentary system; early embryo; gonad; hemolymphoid system gland; and liver. Orthologous to human SIAH1 (siah E3 ubiquitin protein ligase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in whole brain E14.5 (RPKM 8.3), CNS E14 (RPKM 8.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C3; 8 42.1 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (87442450..87472634, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (86720454..86746006, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ubiquitin Ligases Siah1a/2 Control Alveolar Macrophage Functions to Limit Carcinogen-Induced Lung Adenocarcinoma.
Title: Ubiquitin Ligases Siah1a/2 Control Alveolar Macrophage Functions to Limit Carcinogen-Induced Lung Adenocarcinoma.
Depletion of KDM3A coincided with the loss of SIAH1 induction and the accumulation of dimethylated H3K9 surrounding the SIAH1 promoter. Interestingly, KDM3A expression was also up-regulated by nutritional stress in a HIF-1alpha dependent manner.
Title: HIF-1α coordinates epigenetic activation of SIAH1 in hepatocytes in response to nutritional stress.
Combination of netrin-1 and SIAH RNAi may prove to be a substantially effective therapy for myocardial infarction.
Title: Central role of SIAH inhibition in DCC-dependent cardioprotection provoked by netrin-1/NO.
The B23 regulates neuronal survival by preventing SIAH1-GAPDH death signaling under stress-induced conditions in the brain.
Title: S-nitrosylation of B23/nucleophosmin by GAPDH protects cells from the SIAH1-GAPDH death cascade.
The present study demonstrated for the first time that zygotic specific miRNA modulates the first cell cleavage through regulating expression of Siah1a.
Title: miR-135A regulates preimplantation embryo development through down-regulation of E3 Ubiquitin Ligase Seven In Absentia Homolog 1A (SIAH1A) expression.
Siah1 protein is required for glucose limitation-induced Cyclin-Dependent Kinase Inhibitor p27 degradation.
Title: Siah1/SIP regulates p27(kip1) stability and cell migration under metabolic stress.
Siah1A (mouse) reduced PHD3 protein levels but Siah1A M180K (a substrate-binding groove mutant) did not alter the PHD3 protein levels suggesting that the Siah substrate-binding groove is important for PHD ubiquitylation and degradation.
Title: Inhibition of Siah ubiquitin ligase function.
analysis of the substrate binding site of Siah ubiquitin ligase
Title: Elucidation of the substrate binding site of Siah ubiquitin ligase.
Results from loss-of-function experiments do not support a general role for Siah genes in p53-mediated responses or mitosis.
Title: Normal p53 function in primary cells deficient for Siah genes.
X ray structure: Siah ubiquitin ligase is structurally related to TRAF and modulates TNF-alpha signaling
Title: Siah ubiquitin ligase is structurally related to TRAF and modulates TNF-alpha signaling.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Z19579 (e-PCR)
- UniSTS:163443

MARC_14423-14424:1010076472:1 (e-PCR)
- UniSTS:267663

MARC_14423-14424:1027951438:1 (e-PCR)
- UniSTS:267663

AA982064 (e-PCR)
- UniSTS:159902

AI853500 (e-PCR)
- UniSTS:165593

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables disordered domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin conjugating enzyme binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	TAS Traceable Author Statement more info	PubMed
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within male meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in neuron apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within post-embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein destabilization	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of beta-catenin destruction complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase SIAH1A

Names: RING-type E3 ubiquitin transferase SIAH1A; mSiah-1a; seven in absentia 1A; seven in absentia homolog 1a; siah-1a

NP_033198.1

EC 2.3.2.27

XP_006530847.3

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009172.2 → NP_033198.1 E3 ubiquitin-protein ligase SIAH1A

See identical proteins and their annotated locations for NP_033198.1

Status: VALIDATED

Source sequence(s)

AA420282, AC142211, BC046317, BE634662

Consensus CDS

CCDS22504.1

UniProtKB/Swiss-Prot

P61092, Q06984, Q06985, Q7TPV6

Related

ENSMUSP00000044123.5, ENSMUST00000045296.6

Conserved Domains (2) summary

cd16751
Location:39 → 78

RING-HC_SIAH1; RING finger, HC subclass, found in seven in absentia homolog 1 (SIAH1) and similar proteins

pfam03145
Location:82 → 278

Sina; Seven in absentia protein family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

87442450..87472634 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006530784.5 → XP_006530847.3 E3 ubiquitin-protein ligase SIAH1A isoform X1

Conserved Domains (2) summary

cd16751
Location:112 → 151

RING-HC_SIAH1; RING finger, HC subclass, found in seven in absentia homolog 1 (SIAH1) and similar proteins

pfam03145
Location:155 → 351

Sina; Seven in absentia protein family