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    Arhgef25 Rho guanine nucleotide exchange factor 25 [ Mus musculus (house mouse) ]

    Gene ID: 52666, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Arhgef25provided by MGI
    Official Full Name
    Rho guanine nucleotide exchange factor 25provided by MGI
    Primary source
    MGI:MGI:1277173
    See related
    Ensembl:ENSMUSG00000019467 AllianceGenome:MGI:1277173
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    GEFT; D10Ertd610e; 2410008H17Rik
    Summary
    Predicted to enable guanyl-nucleotide exchange factor activity. Predicted to be involved in axon guidance. Located in myofibril and plasma membrane. Is expressed in several structures, including brain; eye; gut; metanephros; and otocyst. Orthologous to human ARHGEF25 (Rho guanine nucleotide exchange factor 25). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Broad expression in limb E14.5 (RPKM 49.4), bladder adult (RPKM 39.6) and 25 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Arhgef25 in Genome Data Viewer
    Location:
    10 D3; 10 74.5 cM
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (127018390..127025952, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (127182521..127190083, complement)

    Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 51819 Neighboring gene beta-1,4-N-acetyl-galactosaminyl transferase 1 Neighboring gene CapStarr-seq enhancer MGSCv37_chr10:126608171-126608358 Neighboring gene solute carrier family 26, member 10 Neighboring gene RIKEN cDNA F420014N23 gene Neighboring gene deltex 3, E3 ubiquitin ligase Neighboring gene phosphatidylinositol-5-phosphate 4-kinase, type II, gamma

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT. Bryan BA, et al. Mol Cell Biol, 2005 Dec. PMID 16314529, Free PMC Article
    2. p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility. Momotani K, et al. Circ Res, 2011 Oct 14. PMID 21885830, Free PMC Article
    3. GEFT, A Rho family guanine nucleotide exchange factor, regulates lens differentiation through a Rac1-mediated mechanism. Mitchell DC, et al. Curr Mol Med, 2011 Aug. PMID 21663592
    4. Geft is dispensable for the development of the second heart field. Fan X, et al. BMB Rep, 2012 Mar. PMID 22449701
    5. p63RhoGEF regulates auto- and paracrine signaling in cardiac fibroblasts. Ongherth A, et al. J Mol Cell Cardiol, 2015 Nov. PMID 26392029

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. In cultured neonatal cardiac fibroblasts, p63RhoGEF regulated the angiotensin II (Ang II)-dependent RhoA activation, the activation of the serum response factor, and the expression and secretion of CTGF.
      Title: p63RhoGEF regulates auto- and paracrine signaling in cardiac fibroblasts.
    2. The Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse.
      Title: Geft is dispensable for the development of the second heart field.
    3. Pasteurella multocida toxin prevents osteoblast differentiation by transactivation of the MAP-kinase cascade via the Galpha(q/11)--p63RhoGEF--RhoA axis.
      Title: Pasteurella multocida toxin prevents osteoblast differentiation by transactivation of the MAP-kinase cascade via the Gα(q/11)--p63RhoGEF--RhoA axis.
    4. GEFT regulation of lens differentiation and eye development occurs through a Rac1-dependent mechanism.
      Title: GEFT, A Rho family guanine nucleotide exchange factor, regulates lens differentiation through a Rac1-mediated mechanism.
    5. Signaling through p63RhoGEF provides a novel mechanism for selective regulation of blood pressure.
      Title: p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility.
    6. Bves directly interacts with GEFT and is a novel regulator of the Rac1 and Cdc42 signaling cascades.
      Title: Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity.
    7. This protein induces cell proliferation, transformation, and migration.
      Title: A Rac/Cdc42-specific exchange factor, GEFT, induces cell proliferation, transformation, and migration.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 
    • Gene trapped (1) 
    • Targeted (1)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables guanyl-nucleotide exchange factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables guanyl-nucleotide exchange factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in axon guidance IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in extrinsic component of membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in myofibril IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in sarcomere IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    rho guanine nucleotide exchange factor 25
    Names
    Rho guanine nucleotide exchange factor (GEF) 25
    guanine nucleotide exchange factor GEFT
    p63RhoGEF
    rac/Cdc42/Rho exchange factor GEFT
    rhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001166413.2 → NP_001159885.1  rho guanine nucleotide exchange factor 25 isoform 2

      See identical proteins and their annotated locations for NP_001159885.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.
      Source sequence(s)
      AC144852
      Consensus CDS
      CCDS48715.1
      UniProtKB/Swiss-Prot
      Q3TYF3, Q8R1Q4, Q9CWR0
      UniProtKB/TrEMBL
      A0A0R4J211
      Related
      ENSMUSP00000126339.2, ENSMUST00000167353.3
      Conserved Domains (2) summary
      cd13241
      Location:369 → 511
      PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2
      pfam00621
      Location:194 → 365
      RhoGEF; RhoGEF domain

    2. NM_001358557.2 → NP_001345486.1  rho guanine nucleotide exchange factor 25 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC144852
      Consensus CDS
      CCDS88098.1
      UniProtKB/Swiss-Prot
      Q3TYF3, Q8R1Q4, Q9CWR0
      UniProtKB/TrEMBL
      A0A1W2P6R9
      Related
      ENSMUSP00000151325.2, ENSMUST00000218654.2
      Conserved Domains (2) summary
      cd00160
      Location:163 → 334
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd13241
      Location:339 → 481
      PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

    3. NM_001358558.2 → NP_001345487.1  rho guanine nucleotide exchange factor 25 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC144852
      UniProtKB/Swiss-Prot
      Q3TYF3, Q8R1Q4, Q9CWR0
      Conserved Domains (2) summary
      cd00160
      Location:120 → 291
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd13241
      Location:296 → 438
      PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

    4. NM_001428940.1 → NP_001415869.1  rho guanine nucleotide exchange factor 25 isoform 5

      Status: VALIDATED

      Source sequence(s)
      AC144852
      UniProtKB/Swiss-Prot
      Q3TYF3, Q8R1Q4, Q9CWR0

    5. NM_028027.4 → NP_082303.2  rho guanine nucleotide exchange factor 25 isoform 1

      See identical proteins and their annotated locations for NP_082303.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AC144852
      Consensus CDS
      CCDS24231.1
      UniProtKB/Swiss-Prot
      Q3TYF3, Q8R1Q4, Q9CWR0
      UniProtKB/TrEMBL
      G5E825
      Related
      ENSMUSP00000019611.8, ENSMUST00000019611.15
      Conserved Domains (2) summary
      cd13241
      Location:378 → 520
      PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2
      pfam00621
      Location:203 → 374
      RhoGEF; RhoGEF domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000076.7 Reference GRCm39 C57BL/6J

      Range
      127018390..127025952 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030245177.1 → XP_030101037.1  rho guanine nucleotide exchange factor 25 isoform X3

      Related
      ENSMUST00000219649.2
      Conserved Domains (2) summary
      cd00160
      Location:1 → 87
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd13241
      Location:92 → 234
      PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

    2. XM_006513879.5 → XP_006513942.1  rho guanine nucleotide exchange factor 25 isoform X1

      Conserved Domains (2) summary
      cd00160
      Location:139 → 310
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd13241
      Location:315 → 457
      PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

    3. XM_006513881.3 → XP_006513944.1  rho guanine nucleotide exchange factor 25 isoform X2

      See identical proteins and their annotated locations for XP_006513944.1

      UniProtKB/Swiss-Prot
      Q3TYF3, Q8R1Q4, Q9CWR0
      Conserved Domains (2) summary
      cd00160
      Location:120 → 291
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd13241
      Location:296 → 438
      PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

    RNA

    1. XR_871718.4 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9CWR0.1 GenPept UniProtKB/Swiss-Prot:Q9CWR0

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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