Ube2i ubiquitin-conjugating enzyme E2I [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ube2iprovided by MGI
Official Full Name: ubiquitin-conjugating enzyme E2Iprovided by MGI
Primary source: MGI:MGI:107365
See related: Ensembl:ENSMUSG00000015120 AllianceGenome:MGI:107365
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: UBC9; Ubce9; Ubce2i; 5830467E05Rik
Summary: Enables HLH domain binding activity and SUMO conjugating enzyme activity. Involved in several processes, including negative regulation of transcription by RNA polymerase II; nuclear export; and protein sumoylation. Acts upstream of or within positive regulation of canonical NF-kappaB signal transduction. Located in nuclear body. Part of SUMO ligase complex. Is active in several cellular components, including Schaffer collateral - CA1 synapse; postsynaptic cytosol; and presynaptic cytosol. Is expressed in several structures, including early embryo; forebrain; forelimb bud; limb; and oocyte. Orthologous to human UBE2I (ubiquitin conjugating enzyme E2 I). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 68.8), whole brain E14.5 (RPKM 68.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 12.53 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (25479484..25494965, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (25260510..25275799, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ubc9 regulates the expression of MHC II in dendritic cells to enhance DSS-induced colitis by mediating RBPJ SUMOylation.
Title: Ubc9 regulates the expression of MHC II in dendritic cells to enhance DSS-induced colitis by mediating RBPJ SUMOylation.
UBC9 deficiency enhances immunostimulatory macrophage activation and subsequent antitumor T cell response in prostate cancer.
Title: UBC9 deficiency enhances immunostimulatory macrophage activation and subsequent antitumor T cell response in prostate cancer.
Ube2i deletion in adipocytes causes lipoatrophy in mice.
Title: Ube2i deletion in adipocytes causes lipoatrophy in mice.
T-cell receptor (TCR) signaling promotes the assembly of RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via PKC-theta-mediated phosphorylation of RanGAP1.
Title: T-cell receptor (TCR) signaling promotes the assembly of RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via PKC-θ-mediated phosphorylation of RanGAP1.
Adipose-specific knockout of ubiquitin-conjugating enzyme E2L6 (Ube2l6) reduces diet-induced obesity, insulin resistance, and hepatic steatosis.
Title: Adipose-specific knockout of ubiquitin-conjugating enzyme E2L6 (Ube2l6) reduces diet-induced obesity, insulin resistance, and hepatic steatosis.
Cutting Edge: Hypoxia-Induced Ubc9 Promoter Hypermethylation Regulates IL-17 Expression in Ulcerative Colitis.
Title: Cutting Edge: Hypoxia-Induced Ubc9 Promoter Hypermethylation Regulates IL-17 Expression in Ulcerative Colitis.
The SUMO Conjugase Ubc9 Protects Dopaminergic Cells from Cytotoxicity and Enhances the Stability of alpha-Synuclein in Parkinson's Disease Models.
Title: The SUMO Conjugase Ubc9 Protects Dopaminergic Cells from Cytotoxicity and Enhances the Stability of α-Synuclein in Parkinson's Disease Models.
An unanticipated tumor-suppressive role of the SUMO pathway in the intestine unveiled by Ubc9 haploinsufficiency.
Title: An unanticipated tumor-suppressive role of the SUMO pathway in the intestine unveiled by Ubc9 haploinsufficiency.
Loss of ubiquitin-conjugating enzyme E2 (Ubc9) in macrophages exacerbates multiple low-dose streptozotocin-induced diabetes by attenuating M2 macrophage polarization.
Title: Loss of ubiquitin-conjugating enzyme E2 (Ubc9) in macrophages exacerbates multiple low-dose streptozotocin-induced diabetes by attenuating M2 macrophage polarization.
Transcriptomic profiling of ovaries suggests that loss of oocyte Ube2i caused defects in both oocyte- and granulosa cell-expressed genes, including NOBOX and some of its known target genes.
Title: Loss of the E2 SUMO-conjugating enzyme Ube2i in oocytes during ovarian folliculogenesis causes infertility in mice.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Gene trapped (1)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237581

AI043902 (e-PCR)
- UniSTS:243393

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables HLH domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables RING-like zinc finger domain binding	ISO Inferred from Sequence Orthology more info
enables SUMO conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables SUMO conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables SUMO conjugating enzyme activity	ISO Inferred from Sequence Orthology more info
enables SUMO transferase activity	ISO Inferred from Sequence Orthology more info
enables bHLH transcription factor binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables ionotropic glutamate receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small protein activating enzyme binding	ISO Inferred from Sequence Orthology more info
enables transcription coregulator binding	ISO Inferred from Sequence Orthology more info
enables transcription factor binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in nuclear export	EXP Inferred from Experiment more info	PubMed
involved_in nuclear export	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of SUMO transferase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of canonical NF-kappaB signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in protein modification process	TAS Traceable Author Statement more info	PubMed
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein sumoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein sumoylation	IEA Inferred from Electronic Annotation more info
involved_in protein sumoylation	ISO Inferred from Sequence Orthology more info
involved_in protein sumoylation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in PML body	ISO Inferred from Sequence Orthology more info
part_of SUMO ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of SUMO ligase complex	ISO Inferred from Sequence Orthology more info
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in fibrillar center	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear body	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
part_of nuclear pore	NAS Non-traceable Author Statement more info	PubMed
is_active_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of transferase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: SUMO-conjugating enzyme UBC9

Names: RING-type E3 SUMO transferase UBC9; SUMO-protein ligase; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase I

NP_001171080.1

EC 6.3.2.19

NP_001171081.1

EC 6.3.2.19

NP_001344623.1

EC 6.3.2.19

NP_035795.1

EC 6.3.2.19

XP_006524112.1

EC 6.3.2.19

XP_036016378.1

EC 6.3.2.19

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001177609.1 → NP_001171080.1 SUMO-conjugating enzyme UBC9

See identical proteins and their annotated locations for NP_001171080.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) represents the longest transcript. Variants 1-4 encode the same protein.

Source sequence(s)

AK150575, AK166657, AK166930

Consensus CDS

CCDS28513.1

UniProtKB/Swiss-Prot

P63280, Q6RUT3

UniProtKB/TrEMBL

A0AAA9WWE2

Related

ENSMUSP00000134261.2, ENSMUST00000173084.9

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme
NM_001177610.1 → NP_001171081.1 SUMO-conjugating enzyme UBC9

See identical proteins and their annotated locations for NP_001171081.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1-4 encode the same protein.

Source sequence(s)

AC122454, AK166930

Consensus CDS

CCDS28513.1

UniProtKB/Swiss-Prot

P63280, Q6RUT3

UniProtKB/TrEMBL

A0AAA9WWE2

Related

ENSMUSP00000134350.2, ENSMUST00000174031.9

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme
NM_001357694.1 → NP_001344623.1 SUMO-conjugating enzyme UBC9

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1-4 encode the same protein.

Source sequence(s)

AC122454, CD776278

Consensus CDS

CCDS28513.1

UniProtKB/Swiss-Prot

P63280, Q6RUT3

UniProtKB/TrEMBL

A0AAA9WWE2

Related

ENSMUSP00000134161.3, ENSMUST00000173621.9

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme
NM_011665.4 → NP_035795.1 SUMO-conjugating enzyme UBC9

See identical proteins and their annotated locations for NP_035795.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1-4 encode the same protein.

Source sequence(s)

AK005058, BY074880

Consensus CDS

CCDS28513.1

UniProtKB/Swiss-Prot

P63280, Q6RUT3

UniProtKB/TrEMBL

A0AAA9WWE2

Related

ENSMUSP00000134491.2, ENSMUST00000173713.9

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme