Lcmt1 leucine carboxyl methyltransferase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lcmt1provided by MGI
Official Full Name: leucine carboxyl methyltransferase 1provided by MGI
Primary source: MGI:MGI:1353593
See related: Ensembl:ENSMUSG00000030763 AllianceGenome:MGI:1353593
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Lcmt; LCMT-1
Summary: Enables protein methyltransferase activity. Acts upstream of or within protein methylation and regulation of glucose metabolic process. Located in cytosol. Is expressed in olfactory cortex mantle layer. Orthologous to human LCMT1 (leucine carboxyl methyltransferase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E18 (RPKM 17.1), cortex adult (RPKM 13.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F3; 7 67.42 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (122977026..123029581)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (123377803..123430358)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hepatic leucine carboxyl methyltransferase 1 (LCMT1) contributes to high fat diet-induced glucose intolerance through regulation of glycogen metabolism.
Title: Hepatic leucine carboxyl methyltransferase 1 (LCMT1) contributes to high fat diet-induced glucose intolerance through regulation of glycogen metabolism.
Leucine Carboxyl Methyltransferase 1 Overexpression Protects Against Cognitive and Electrophysiological Impairments in Tg2576 APP Transgenic Mice.
Title: Leucine Carboxyl Methyltransferase 1 Overexpression Protects Against Cognitive and Electrophysiological Impairments in Tg2576 APP Transgenic Mice.
Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice.
Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice.
Loss of Lcmt1 expression is associated with severe defects in fetal liver hematopoiesis.
Title: Global loss of leucine carboxyl methyltransferase-1 causes severe defects in fetal liver hematopoiesis.
LCMT-1 homozygous knock-out MEFs exhibited hyperphosphorylation of HDAC3, a reported target of the methylation-dependent PP4R1-PP4c complex. Collectively, our data suggest that LCMT-1 coordinately regulates the carboxyl methylation of PP2A-related phosphatases and, consequently, their holoenzyme assembly and function.
Title: Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
In LCMT1 deficiency enzyme activity and methylation of PP2A are reduced in a coordinate fashion, suggesting that LCMT1 is the only PP2A methyltransferase.
Title: Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.
Enhanced expression of LCMT1 in cultured neuroblastoma cells, which increases endogenous methylated catalytic C subunit and Balpha levels, induces changes in F-actin organization.
Title: Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.
LCMT-1 is important for normal progression through mitosis and cell survival and is essential for embryonic development
Title: Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (3) 1 citation
Targeted (1)
Transgenic (1)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein C-terminal carboxyl O-methyltransferase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables protein C-terminal leucine carboxyl O-methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within C-terminal protein methylation	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within negative regulation of protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein methylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein methylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of glucose metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic cell cycle spindle assembly checkpoint	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of mitotic cell cycle spindle assembly checkpoint	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: leucine carboxyl methyltransferase 1

NP_001341979.1

EC 2.1.1.233

NP_079580.2

EC 2.1.1.233

XP_030098526.1

EC 2.1.1.233

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001355050.1 → NP_001341979.1 leucine carboxyl methyltransferase 1 isoform 2

Status: VALIDATED

Source sequence(s)

AC121956

Conserved Domains (1) summary

cl17173
Location:3 → 59

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
NM_025304.3 → NP_079580.2 leucine carboxyl methyltransferase 1 isoform 1

See identical proteins and their annotated locations for NP_079580.2

Status: VALIDATED

Source sequence(s)

AK003424, BB686087, BU571243

Consensus CDS

CCDS40118.1

UniProtKB/TrEMBL

A0A0U1RNF2, A2RTH5

Related

ENSMUSP00000033025.6, ENSMUST00000033025.7

Conserved Domains (1) summary

pfam04072
Location:24 → 215

LCM; Leucine carboxyl methyltransferase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

122977026..123029581

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030242666.1 → XP_030098526.1 leucine carboxyl methyltransferase 1 isoform X1

Conserved Domains (1) summary

cl17173
Location:3 → 59

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...