Slc32a1 solute carrier family 32 (GABA vesicular transporter), member 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slc32a1provided by MGI
Official Full Name: solute carrier family 32 (GABA vesicular transporter), member 1provided by MGI
Primary source: MGI:MGI:1194488
See related: Ensembl:ENSMUSG00000037771 AllianceGenome:MGI:1194488
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: VGAT; Viaat
Summary: Enables gamma-aminobutyric acid:proton antiporter activity and glycine:proton antiporter activity. Involved in gamma-aminobutyric acid import and glycine transport. Located in several cellular components, including cone cell pedicle; dendrite terminus; and inhibitory synapse. Is expressed in several structures, including cardiovascular system; central nervous system; eye; metanephros; and skeleton. Used to study temporal lobe epilepsy. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy and generalized epilepsy with febrile seizures plus. Orthologous to human SLC32A1 (solute carrier family 32 member 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cerebellum adult (RPKM 56.5), frontal lobe adult (RPKM 42.0) and 5 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H1; 2 78.72 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (158449158..158457667)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (158607260..158615747)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tfap2b acts in GABAergic neurons to control sleep in mice.
Title: Tfap2b acts in GABAergic neurons to control sleep in mice.
De Novo Missense Variants in SLC32A1 Cause a Developmental and Epileptic Encephalopathy Due to Impaired GABAergic Neurotransmission.
Title: De Novo Missense Variants in SLC32A1 Cause a Developmental and Epileptic Encephalopathy Due to Impaired GABAergic Neurotransmission.
Posterodorsal Medial Amygdala Regulation of Female Social Behavior: GABA versus Glutamate Projections.
Title: Posterodorsal Medial Amygdala Regulation of Female Social Behavior: GABA versus Glutamate Projections.
Characterization of kindled VGAT-Cre mice as a new animal model of temporal lobe epilepsy.
Title: Characterization of kindled VGAT-Cre mice as a new animal model of temporal lobe epilepsy.
the formation of inhibitory and excitatory synapses onto excitatory neurons requires Vgat in interneurons
Title: Regulation of Synapse Development by Vgat Deletion from ErbB4-Positive Interneurons.
Intense VIAAT immunoreactivity was found largely confined to the distal tubule epithelia, especially distinct in the inner medulla, although the immunoreactivity was discerned more or less in all tubules and glomeruli
Title: Expression and localization of VIAAT in distal uriniferous tubular epithelium of mouse.
Study investigated VIAAT expression during development in: 4 different wildtype-strains, 1 transgenic mouse line with congenital deafness and 1 mouse line with cochlear ablation. Results identify an activity-regulated gradient of VIAAT in the superior olivary complex of NRMI mice. Its absence in other mouse strains adds a novel layer of strain-specific features in the auditory system.
Title: Activity-dependent formation of a vesicular inhibitory amino acid transporter gradient in the superior olivary complex of NMRI mice.
GABAergic SVs that lacked the vesicular GABA transporter (VGAT) did not show the pH overshoot and acidified further to approximately 6.0. Comparison of luminal pH dynamics in the presence or absence of VGAT showed that VGAT operates as a GABA/H(+) exchanger, which is continuously required to offset GABA leakage
Title: Unique pH dynamics in GABAergic synaptic vesicles illuminates the mechanism and kinetics of GABA loading.
The observed changes in gene and protein expression of vesicular GABA transporter, regional architecture, and morphology of mouse auditory forebrain may relate to-and to some extent enable-the emergence of mature sound-evoked activity patterns.
Title: Differential maturation of vesicular glutamate and GABA transporter expression in the mouse auditory forebrain during the first weeks of hearing.
the AD-like tau accumulation induces anxiety through disrupting miR92a-vGAT-GABA signaling, which reveals molecular mechanisms underlying the anxiety behavior in AD patients and potentially leads to the development of new therapeutics for tauopathies.
Title: Correcting miR92a-vGAT-Mediated GABAergic Dysfunctions Rescues Human Tau-Induced Anxiety in Mice.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6)
Targeted (11) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Slc32a1 (e-PCR), detects polymorphism
- UniSTS:471076

Slc32a1 (e-PCR), detects polymorphism
- UniSTS:525657

Slc32a1 (e-PCR), detects polymorphism
- UniSTS:507228

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables gamma-aminobutyric acid transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables gamma-aminobutyric acid:proton antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables gamma-aminobutyric acid:proton antiporter activity	ISO Inferred from Sequence Orthology more info
enables gamma-aminobutyric acid:proton antiporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables glycine transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycine transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables glycine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables glycine:proton antiporter activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in beta-alanine transport	ISO Inferred from Sequence Orthology more info
involved_in beta-alanine transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in gamma-aminobutyric acid import	IDA Inferred from Direct Assay more info	PubMed
involved_in gamma-aminobutyric acid import	ISO Inferred from Sequence Orthology more info
involved_in gamma-aminobutyric acid import	ISS Inferred from Sequence or Structural Similarity more info
involved_in gamma-aminobutyric acid transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in gamma-aminobutyric acid transport	ISO Inferred from Sequence Orthology more info
involved_in glycine transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within glycine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in glycine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in glycine transport	ISO Inferred from Sequence Orthology more info
involved_in hippocampus development	IEA Inferred from Electronic Annotation more info
involved_in neurotransmitter loading into synaptic vesicle	IBA Inferred from Biological aspect of Ancestor more info
involved_in neurotransmitter loading into synaptic vesicle	IEA Inferred from Electronic Annotation more info
involved_in neurotransmitter loading into synaptic vesicle	ISO Inferred from Sequence Orthology more info
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in GABA-ergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in GABA-ergic synapse	ISO Inferred from Sequence Orthology more info
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell tip	IDA Inferred from Direct Assay more info	PubMed
located_in cone cell pedicle	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in dendrite terminus	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite terminus	IDA Inferred from Direct Assay more info	PubMed
located_in inhibitory synapse	IDA Inferred from Direct Assay more info	PubMed
located_in inhibitory synapse	ISO Inferred from Sequence Orthology more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in neuron projection terminus	IBA Inferred from Biological aspect of Ancestor more info
located_in neuron projection terminus	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection terminus	ISO Inferred from Sequence Orthology more info	PubMed
located_in plasma membrane	IC Inferred by Curator more info	PubMed
located_in presynapse	ISO Inferred from Sequence Orthology more info
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info	PubMed
located_in synaptic vesicle	ISO Inferred from Sequence Orthology more info	PubMed
located_in synaptic vesicle	ISS Inferred from Sequence or Structural Similarity more info
is_active_in synaptic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic vesicle membrane	IEA Inferred from Electronic Annotation more info
is_active_in synaptic vesicle membrane	ISO Inferred from Sequence Orthology more info
located_in synaptic vesicle membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: vesicular inhibitory amino acid transporter

Names: GABA and glycine transporter; mVGAT; mVIAAT; solute carrier family 32 member 1; vasicular inhibitory amino acid transporter 10D; vesicular GABA and glycine transporter; vesicular GABA transporter a form

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001421187.1 → NP_001408116.1 vesicular inhibitory amino acid transporter

Status: VALIDATED

Source sequence(s)

AL663091

UniProtKB/Swiss-Prot

O35633

UniProtKB/TrEMBL

Q49S98
NM_009508.3 → NP_033534.2 vesicular inhibitory amino acid transporter

See identical proteins and their annotated locations for NP_033534.2

Status: VALIDATED

Source sequence(s)

AL663091

Consensus CDS

CCDS38309.1

UniProtKB/Swiss-Prot

O35633

UniProtKB/TrEMBL

Q49S98

Related

ENSMUSP00000036299.5, ENSMUST00000045738.5

Conserved Domains (1) summary

pfam01490
Location:115 → 512

Aa_trans; Transmembrane amino acid transporter protein