Ppp1r12a protein phosphatase 1, regulatory subunit 12A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ppp1r12aprovided by MGI
Official Full Name: protein phosphatase 1, regulatory subunit 12Aprovided by MGI
Primary source: MGI:MGI:1309528
See related: Ensembl:ENSMUSG00000019907 AllianceGenome:MGI:1309528
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mypt1; D10Ertd625e; 1200015F06Rik; 5730577I22Rik
Summary: Predicted to enable several functions, including 14-3-3 protein binding activity; myosin phosphatase regulator activity; and protein kinase binding activity. Acts upstream of or within cellular response to xenobiotic stimulus; protein dephosphorylation; and regulation of nucleocytoplasmic transport. Located in A band and Z disc. Is expressed in several structures, including genital tubercle epithelium; sciatic nerve; telencephalon; urethra epithelium; and urothelium. Orthologous to human PPP1R12A (protein phosphatase 1 regulatory subunit 12A). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in bladder adult (RPKM 144.2), CNS E11.5 (RPKM 14.0) and 11 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 D1; 10 56.33 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (107997913..108115846)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (108162092..108279985)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MYPT1[SMKO] Mice Function as a Novel Spontaneous Age- and Hypertension-Dependent Animal Model of CSVD.
Title: MYPT1(SMKO) Mice Function as a Novel Spontaneous Age- and Hypertension-Dependent Animal Model of CSVD.
Gallbladder dysfunction caused by MYPT1 ablation triggers cholestasis-induced hepatic fibrosis in mice.
Title: Gallbladder dysfunction caused by MYPT1 ablation triggers cholestasis-induced hepatic fibrosis in mice.
MYPT1-PP1beta phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis.
Title: MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis.
MYPT1 reduction is a pathogenic factor of erectile dysfunction.
Title: MYPT1 reduction is a pathogenic factor of erectile dysfunction.
Tissue-specific expression of myosin phosphatase subunits and isoforms in smooth muscle of mice and humans.
Title: Tissue-specific expression of myosin phosphatase subunits and isoforms in smooth muscle of mice and humans.
Editing of the myosin phosphatase regulatory subunit suppresses angiotensin II induced hypertension via sensitization to nitric oxide mediated vasodilation.
Title: Editing of the myosin phosphatase regulatory subunit suppresses angiotensin II induced hypertension via sensitization to nitric oxide mediated vasodilation.
O-GlcNAc modification of MYPT1 modulates lysophosphatidic acid-induced cell contraction in fibroblasts.
Title: O-GlcNAc modification of MYPT1 modulates lysophosphatidic acid-induced cell contraction in fibroblasts.
MYPT1 Down-regulation by Lipopolysaccharide-SIAH1/2 E3 Ligase-Ubiquitin-Proteasomal Degradation Contributes to Colonic Obstruction of Hirschsprung Disease.
Title: MYPT1 Down-regulation by Lipopolysaccharide-SIAH1/2 E3 Ligase-Ubiquitin-Proteasomal Degradation Contributes to Colonic Obstruction of Hirschsprung Disease.
Augmented contractility of murine femoral arteries in a streptozotocin diabetes model is related to increased threonine phosphorylation of MYPT1.
Title: Augmented contractility of murine femoral arteries in a streptozotocin diabetes model is related to increased phosphorylation of MYPT1.
The present results suggested that ROCK1 inhibitor could attenuate mechanical stretchinduced lung endothelial injury and the phosphorylation of MYPT1 in vivo and in vitro. Collectively, the present findings indicated that upregulation of SPHK1 may contribute to VALI in a twohit model.
Title: Upregulation of sphingosine kinase 1 contributes to ventilator-associated lung injury in a two-hit model.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Gene trapped (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MHAa53c6.seq (e-PCR)
- UniSTS:125196

D10Ertd625e (e-PCR), detects polymorphism
- UniSTS:167023

AV099298 (e-PCR)
- UniSTS:180116

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 14-3-3 protein binding	ISO Inferred from Sequence Orthology more info
enables 14-3-3 protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables enzyme inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables enzyme inhibitor activity	ISO Inferred from Sequence Orthology more info
enables enzyme inhibitor activity	ISS Inferred from Sequence or Structural Similarity more info
enables myosin phosphatase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatase regulator activity	ISO Inferred from Sequence Orthology more info
enables phosphatase regulator activity	ISS Inferred from Sequence or Structural Similarity more info
enables phosphoprotein phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cellular response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in centrosome cycle	ISO Inferred from Sequence Orthology more info
involved_in centrosome cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in mitotic cell cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of catalytic activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of catalytic activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in neuron projection morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein dephosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell adhesion	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of nucleocytoplasmic transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in A band	IBA Inferred from Biological aspect of Ancestor more info
located_in A band	IDA Inferred from Direct Assay more info	PubMed
part_of PTW/PP1 phosphatase complex	ISO Inferred from Sequence Orthology more info
part_of PTW/PP1 phosphatase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in contractile muscle fiber	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in stress fiber	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein phosphatase 1 regulatory subunit 12A

Names: myosin phosphatase target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase 1, regulatory (inhibitor) subunit 12A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001368736.1 → NP_001355665.1 protein phosphatase 1 regulatory subunit 12A isoform 1

Status: VALIDATED

Source sequence(s)

AC139335

Consensus CDS

CCDS88083.1

UniProtKB/Swiss-Prot

Q05A74, Q8CBV2, Q99NB6, Q9DBR7

Related

ENSMUSP00000151842.2, ENSMUST00000219263.2

Conserved Domains (4) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam15898
Location:930 → 1028

PRKG1_interact; cGMP-dependent protein kinase interacting domain
NM_001368737.1 → NP_001355666.1 protein phosphatase 1 regulatory subunit 12A isoform 3

Status: VALIDATED

Source sequence(s)

AC139335

Conserved Domains (4) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam15898
Location:874 → 972

PRKG1_interact; cGMP-dependent protein kinase interacting domain
NM_027892.3 → NP_082168.1 protein phosphatase 1 regulatory subunit 12A isoform 2

See identical proteins and their annotated locations for NP_082168.1

Status: VALIDATED

Source sequence(s)

AC139335

Consensus CDS

CCDS36052.1

UniProtKB/Swiss-Prot

Q9DBR7

Related

ENSMUSP00000069257.6, ENSMUST00000070663.6

Conserved Domains (5) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:199 → 252

Ank_4; Ankyrin repeats (many copies)

pfam15898
Location:930 → 984

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

107997913..108115846

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006513329.5 → XP_006513392.1 protein phosphatase 1 regulatory subunit 12A isoform X2

See identical proteins and their annotated locations for XP_006513392.1

Conserved Domains (3) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:873 → 971

PRKG1_interact; cGMP-dependent protein kinase interacting domain
XM_006513327.5 → XP_006513390.1 protein phosphatase 1 regulatory subunit 12A isoform X1

Conserved Domains (3) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:930 → 984

PRKG1_interact; cGMP-dependent protein kinase interacting domain