Hyal5 hyaluronoglucosaminidase 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hyal5provided by MGI
Official Full Name: hyaluronoglucosaminidase 5provided by MGI
Primary source: MGI:MGI:1921718
See related: Ensembl:ENSMUSG00000029678 AllianceGenome:MGI:1921718
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4933439A12Rik
Summary: Enables hyalurononglucosaminidase activity. Acts upstream of or within penetration of zona pellucida. Located in acrosomal vesicle and external side of plasma membrane. Orthologous to human SPAM1 (sperm adhesion molecule 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward testis adult (RPKM 24.7) See more
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Genomic context

Location:: 6 A3.1; 6 11.28 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (24857982..24891963)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (24857937..24891959)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A comparative analysis between WT and Spam1/Hyal5 dKO epididymal sperm revealed that the absence of these 2 sperm Hyals resulted in a marked accumulation of sperm on the outside of the cumulus-oocyte complex.
Title: SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect.
The data from this paper suggests that the hyaluronan-degrading activity of the sperm hyaluronidase HYAL5 may not be required for fertilization, at least in the mouse.
Title: Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?
The HYAL5-deficient female mice were fully fertile and yielded normal litter sizes. HYAL5-deficient epididymal sperm is functionally normal. Thus, HYAL5 may be dispensable for fertilization.
Title: Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization.
Mouse SPAM1 and HYAL5 but not HYALP1 proteins exhibit hyaluronidase activity at neutral pH.
Title: Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation

General gene information

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Markers

Hyal5 (e-PCR), detects polymorphism
- UniSTS:546827

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hyalurononglucosaminidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables hyalurononglucosaminidase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IEA Inferred from Electronic Annotation more info
involved_in hyaluronan catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within penetration of zona pellucida	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in acrosomal membrane	IEA Inferred from Electronic Annotation more info
is_active_in acrosomal vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: hyaluronidase-5

Names: hyal-5; hyaluronidase Hyal-5; hyaluronidase PH-20

NP_001404776.1

EC 3.2.1.35

NP_001404777.1

EC 3.2.1.35

NP_001404778.1

EC 3.2.1.35

NP_083233.1

EC 3.2.1.35

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001417847.1 → NP_001404776.1 hyaluronidase-5 isoform a precursor

Status: VALIDATED

Source sequence(s)

AC127559

UniProtKB/Swiss-Prot

Q812F3

UniProtKB/TrEMBL

Q9CUA5

Related

ENSMUSP00000031689.6, ENSMUST00000031689.6
NM_001417848.1 → NP_001404777.1 hyaluronidase-5 isoform b

Status: VALIDATED

Source sequence(s)

AC127559
NM_001417849.1 → NP_001404778.1 hyaluronidase-5 isoform c

Status: VALIDATED

Source sequence(s)

AC127559
NM_028957.3 → NP_083233.1 hyaluronidase-5 isoform a precursor

See identical proteins and their annotated locations for NP_083233.1

Status: VALIDATED

Source sequence(s)

AC127559

Consensus CDS

CCDS19948.1

UniProtKB/Swiss-Prot

Q812F3

UniProtKB/TrEMBL

Q9CUA5

Related

ENSMUSP00000144011.2, ENSMUST00000200968.4

Conserved Domains (1) summary

pfam01630
Location:42 → 369

Glyco_hydro_56; Hyaluronidase