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    gshB glutathione synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947445, updated on 17-Dec-2024

    Summary

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    Official Symbol
    gshB
    Official Full Name
    glutathione synthetase
    Primary source
    ECOCYC:EG10419
    Locus tag
    b2947
    See related
    ASAP:ABE-0009667
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2942; gsh-II
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    Genomic context

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    Sequence:
    NC_000913.3 (3091878..3092828)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-specific endonuclease I Neighboring gene 16S rRNA m(3)U1498 methyltransferase Neighboring gene DUF179 domain-containing protein YqgE Neighboring gene ribonuclease H-like domain-containing nuclease Neighboring gene Type II/IV secretion system family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Homology of Escherichia coli B glutathione synthetase with dihydrofolate reductase in amino acid sequence and substrate binding site. Kato H, et al. J Biochem, 1987 Jan. PMID 3553173
    2. A pseudo-michaelis quaternary complex in the reverse reaction of a ligase: structure of Escherichia coli B glutathione synthetase complexed with ADP, glutathione, and sulfate at 2.0 A resolution. Hara T, et al. Biochemistry, 1996 Sep 17. PMID 8810901
    3. Modification of Escherichia coli B glutathione synthetase with polyethylene glycol for clinical application to enzyme replacement therapy for glutathione deficiency. Inoue Y, et al. Clin Diagn Lab Immunol, 1996 Nov. PMID 8914756, Free PMC Article
    4. Crystal structure of glutathione synthetase at optimal pH: domain architecture and structural similarity with other proteins. Matsuda K, et al. Protein Eng, 1996 Dec. PMID 9010922
    5. Nicked multifunctional loop of glutathione synthetase still protects the catalytic intermediate. Tanaka T, et al. Arch Biochem Biophys, 1997 Mar 1. PMID 9056244

    See all (45) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on complete protein
      Title: Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glutathione synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glutathione synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables glutathione synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in glutathione biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within glutathione biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein homotetramerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    glutathione synthetase
    NP_417422.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3091878..3092828
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417422.1 glutathione synthetase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417422.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7A6VRJ0
      Conserved Domains (1) summary
      PRK05246
      Location:1 → 316
      PRK05246; glutathione synthetase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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