NDT80 transcription factor NDT80 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NDT80
Official Full Name: transcription factor NDT80
Primary source: SGD:S000001166
Locus tag: YHR124W
See related: AllianceGenome:SGD:S000001166; FungiDB:YHR124W; VEuPathDB:YHR124W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription factor activity. Involved in meiotic cell cycle; positive regulation of transcription by RNA polymerase II; and sporulation. Located in nuclear chromosome. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See NDT80 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (356561..358444)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Using genetic and biochemical approaches, this work demonstrates that in the presence of DSBs, activated Mek1 binds to Ndt80 and phosphorylates the transcription factor, thus inhibiting DNA binding and preventing Ndt80's function as a transcriptional activator.
Title: Mek1 coordinates meiotic progression with DNA break repair by directly phosphorylating and inhibiting the yeast pachytene exit regulator Ndt80.
ndt80 transcription of CDC5 results in degradation of Red1, a meiosis-specific protein required for Mek1 activation, thereby abolishing Mek1 activity completely. Elimination of Mek1 kinase activity allows Rad51-mediated repair of any remaining DSBs. In this way, cells do not enter Meiosis I until recombination is complete and all DSBs are repair
Title: Coordination of Double Strand Break Repair and Meiotic Progression in Yeast by a Mek1-Ndt80 Negative Feedback Loop.
The findings suggest that irreversibility of meiotic commitment is a consequence of the NDT80 transcriptional positive feedback loop, which provides the high-level of Ndt80 required for the developmental switch of meiotic commitment.
Title: Positive feedback of NDT80 expression ensures irreversible meiotic commitment in budding yeast.
Our results reveal a role for Cdc5 in meiotic checkpoint control by regulating Ndt80 function
Title: The budding yeast polo-like kinase Cdc5 regulates the Ndt80 branch of the meiotic recombination checkpoint pathway.
nuclear localization of Ndt80 is regulated by the pachytene checkpoint through a cytoplasmic anchor mechanism
Title: Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint.
transient induction of a transcription factor (NDT80) essential for later stages of gametogenesis extends the replicative life span of aged cells
Title: Gametogenesis eliminates age-induced cellular damage and resets life span in yeast.
deletion of NDT80 did not inhibit DNA rereplication
Title: Sic1-induced DNA rereplication during meiosis.
Cdc7 allows expression of NDT80, is necessary for recruitment of monopolin to sister kinetochores, and it is necessary for the reductional segregation occurring at meiosis I.
Title: Cdc7-Dbf4 regulates NDT80 transcription as well as reductional segregation during budding yeast meiosis.
Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor.
Title: Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables sequence-specific DNA binding	HDA more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in sporulation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcription factor NDT80

NP_011992.1

Meiosis-specific transcription factor; required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

356561..358444

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179254.1 → NP_011992.1 TPA: transcription factor NDT80 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011992.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DL74, P38830, Q66R83

UniProtKB/TrEMBL

B3LSN9, C7GR69, C8Z9R7, G2WFJ8, N1P2T5

Conserved Domains (1) summary

pfam05224
Location:105 → 334

NDT80_PhoG; NDT80 / PhoG like DNA-binding family