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    Hs2st1 heparan sulfate 2-O-sulfotransferase 1 [ Mus musculus (house mouse) ]

    Gene ID: 23908, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Hs2st1provided by MGI
    Official Full Name
    heparan sulfate 2-O-sulfotransferase 1provided by MGI
    Primary source
    MGI:MGI:1346049
    See related
    Ensembl:ENSMUSG00000040151 AllianceGenome:MGI:1346049
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    2OST; Hs2st; mKIAA0448
    Summary
    Enables heparan sulfate 2-O-sulfotransferase activity. Involved in heparan sulfate proteoglycan biosynthetic process. Acts upstream of or within several processes, including heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; heparin metabolic process; and ureteric bud formation. Is active in Golgi apparatus. Is expressed in brain and future brain. Orthologous to human HS2ST1 (heparan sulfate 2-O-sulfotransferase 1). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in lung adult (RPKM 8.3), whole brain E14.5 (RPKM 6.1) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Hs2st1 in Genome Data Viewer
    Location:
    3 H2; 3 68.89 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 3 NC_000069.7 (144135462..144275977, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 3 NC_000069.6 (144429701..144570216, complement)

    Chromosome 3 - NC_000069.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_09177 Neighboring gene predicted gene, 54186 Neighboring gene STARR-seq mESC enhancer starr_09178 Neighboring gene 60S ribosomal protein L21 pseudogene Neighboring gene STARR-positive B cell enhancer mm9_chr3:144193966-144194266 Neighboring gene predicted gene 43560 Neighboring gene STARR-seq mESC enhancer starr_09180 Neighboring gene CapStarr-seq enhancer MGSCv37_chr3:144211269-144211545 Neighboring gene predicted gene, 46829 Neighboring gene STARR-seq mESC enhancer starr_09181 Neighboring gene CapStarr-seq enhancer MGSCv37_chr3:144233322-144233673 Neighboring gene selenoprotein F Neighboring gene STARR-seq mESC enhancer starr_09182 Neighboring gene ribosomal protein L9, pseudogene 8

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Molecular cloning and expression of Chinese hamster ovary cell heparan-sulfate 2-sulfotransferase. Kobayashi M, et al. J Biol Chem, 1997 May 23. PMID 9153262
    2. Hs2st mediated kidney mesenchyme induction regulates early ureteric bud branching. Shah MM, et al. Dev Biol, 2010 Mar 15. PMID 20059993, Free PMC Article
    3. Substrate specificity of the heparan sulfate hexuronic acid 2-O-sulfotransferase. Rong J, et al. Biochemistry, 2001 May 8. PMID 11331020
    4. Specific modification of heparan sulphate is required for normal cerebral cortical development. McLaughlin D, et al. Mech Dev, 2003 Dec. PMID 14654220
    5. Role of heparan sulfate-2-O-sulfotransferase in the mouse. Merry CL, et al. Biochim Biophys Acta, 2002 Dec 19. PMID 12417414

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Chondrocytes respond to an altered heparan sulfate composition with distinct changes of heparan sulfate structure and increased levels of chondroitin sulfate.
      Title: Chondrocytes respond to an altered heparan sulfate composition with distinct changes of heparan sulfate structure and increased levels of chondroitin sulfate.
    2. The specific sulfation modification of the cell surface and extracellular carbohydrate heparan sulfate (HS) performed by Hs2st suppresses the supply of translocation signals to astroglial precursors by a non-cell-autonomous mechanism. Hs2st modification selectively facilitates a physical interaction between Fgf17 and HS and suppresses Fgf17 protein levels in vivo, suggesting that Hs2st acts selectively on Fgf17 signaling.
      Title: Heparan Sulfate Sulfation by Hs2st Restricts Astroglial Precursor Somal Translocation in Developing Mouse Forebrain by a Non-Cell-Autonomous Mechanism.
    3. Inactivation of uronyl 2-O-sulfotransferase in endothelial cells (Hs2st), an enzyme that acts downstream from Ndst1, results in enhanced neutrophil recruitment in several models of acute inflammation.
      Title: Inactivation of heparan sulfate 2-O-sulfotransferase accentuates neutrophil infiltration during acute inflammation in mice.
    4. Plasma lipoprotein clearance depends on specific subclasses of sulfate groups and not on overall charge of the chains.
      Title: Heparan sulfate 2-O-sulfotransferase is required for triglyceride-rich lipoprotein clearance.
    5. mice homozygous for a null mutation in Hs2st indicated reduction in the thickness of the embryonic cerebral cortex; critical role for 2-O-sulphation of heparan sulphate proteoglycan in regulating cell proliferation during cerebral cortex development
      Title: Specific modification of heparan sulphate is required for normal cerebral cortical development.
    6. Hs2st and/or Hs6st1 expression coincides with Slit expression domains at locations where retinal ganglion cell(RGC) axons make navigation errors in Hs2st-/- and Hs6st1-/- mutants, and Hs6st1-/-RGC axons are less sensitive to Slit2 repulsion.
      Title: Heparan sulphation patterns generated by specific heparan sulfotransferase enzymes direct distinct aspects of retinal axon guidance at the optic chiasm.
    7. The phenotype of Hs2st(-/-) mutant kidneys suggests that signalling between two tissues, ureteric bud and metanephric mesenchyme, is disrupted.
      Title: Heparan sulfate 2-O-sulfotransferase (Hs2st) and mouse development.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables heparan sulfate 2-O-sulfotransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables heparan sulfate 2-O-sulfotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heparan sulfate 2-O-sulfotransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables heparan sulfate 2-O-sulfotransferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within gene expression IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in heparan sulfate proteoglycan biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in heparan sulfate proteoglycan biosynthetic process, enzymatic modification IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within heparin metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within ureteric bud formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in Golgi apparatus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in Golgi membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    heparan sulfate 2-O-sulfotransferase 1
    Names
    2-O-sulfotransferase
    NP_001342163.1
    NP_035958.3
    XP_006501513.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001355234.1 → NP_001342163.1  heparan sulfate 2-O-sulfotransferase 1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC123880, AC159976, BC059008
      Conserved Domains (1) summary
      pfam03567
      Location:76 → 327
      Sulfotransfer_2; Sulfotransferase family

    2. NM_011828.4 → NP_035958.3  heparan sulfate 2-O-sulfotransferase 1 isoform 2

      See identical proteins and their annotated locations for NP_035958.3

      Status: VALIDATED

      Source sequence(s)
      AC123880, AC159976, BC059008
      Consensus CDS
      CCDS17883.1
      UniProtKB/Swiss-Prot
      O88464, Q3TNP7, Q8R3H7, Q9JLK2
      Related
      ENSMUSP00000043066.8, ENSMUST00000043325.9
      Conserved Domains (1) summary
      pfam03567
      Location:76 → 326
      Sulfotransfer_2; Sulfotransferase family

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000069.7 Reference GRCm39 C57BL/6J

      Range
      144135462..144275977 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006501450.5 → XP_006501513.1  heparan sulfate 2-O-sulfotransferase 1 isoform X1

      Conserved Domains (1) summary
      pfam03567
      Location:18 → 269
      Sulfotransfer_2; Sulfotransferase family
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8R3H7.2 GenPept UniProtKB/Swiss-Prot:Q8R3H7

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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