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    Naca nascent polypeptide-associated complex alpha polypeptide [ Mus musculus (house mouse) ]

    Gene ID: 17938, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Nacaprovided by MGI
    Official Full Name
    nascent polypeptide-associated complex alpha polypeptideprovided by MGI
    Primary source
    MGI:MGI:106095
    See related
    Ensembl:ENSMUSG00000061315 AllianceGenome:MGI:106095
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    skNAC; Gm1878; mKIAA0363
    Summary
    Enables TBP-class protein binding activity and transcription coactivator activity. Acts upstream of or within myoblast migration. Located in cytoplasm and nucleus. Is expressed in several structures, including aorta; genitourinary system; gut; liver; and spleen. Orthologous to human NACA (nascent polypeptide associated complex subunit alpha). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in heart adult (RPKM 34.3), CNS E11.5 (RPKM 24.7) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Naca in Genome Data Viewer
    Location:
    10 D3; 10 76.39 cM
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (127869144..127884506)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (128035346..128048637)

    Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E9812 Neighboring gene small nuclear ribonucleoprotein D2 pseudogene Neighboring gene DNA primase, p49 subunit Neighboring gene STARR-seq mESC enhancer starr_28254 Neighboring gene STARR-positive B cell enhancer ABC_E5198 Neighboring gene STARR-positive B cell enhancer ABC_E10658 Neighboring gene prostaglandin E synthase 3 Neighboring gene predicted gene, 47438 Neighboring gene STARR-positive B cell enhancer ABC_E25 Neighboring gene small nucleolar RNA, C/D box 59A Neighboring gene ATP synthase F1 subunit beta

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH. St-Arnaud R, et al. Int J Mol Sci, 2022 Jan 15. PMID 35055125, Free PMC Article
    2. GSK3 beta-dependent phosphorylation of the alpha NAC coactivator regulates its nuclear translocation and proteasome-mediated degradation. Quélo I, et al. Biochemistry, 2004 Mar 16. PMID 15005626
    3. Nuclear alpha NAC influences bone matrix mineralization and osteoblast maturation in vivo. Meury T, et al. Mol Cell Biol, 2010 Jan. PMID 19884350, Free PMC Article
    4. Fine mapping of the alpha-NAC gene (Naca) to the distal end (D2-D3) of mouse chromosome 10. Yotov WV, et al. Mamm Genome, 1996 Mar. PMID 8833254
    5. Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): implications for a specific role in mammalian myoblast differentiation. Berger F, et al. Eur J Cell Biol, 2012 Feb. PMID 22154550

    See all (64) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The ribosomal chaperone NACA recruits PHD2 to cotranslationally modify HIF-alpha.
      Title: The ribosomal chaperone NACA recruits PHD2 to cotranslationally modify HIF-α.
    2. The data suggest that skNAC and Smyd1 might be major players in the processes of myofibrillogenesis and sarcomerogenesis, probably specifically at later stages.
      Title: siRNA-mediated inhibition of skNAC and Smyd1 expression disrupts myofibril organization: Immunofluorescence and electron microscopy study in C2C12 cells.
    3. Data show that reduction of alpha chain of nascent polypeptide associated complex (alphaNAC) levels decreased basal expression of Low density lipoprotein receptor-Related Protein 6 (Lrp6) by 30%.
      Title: Lrp6 is a target of the PTH-activated αNAC transcriptional coregulator.
    4. the skNAC-Smyd1 complex is involved in transcriptional regulation both via the control of histone methylation and histone (de)acetylation.
      Title: skNAC and Smyd1 in transcriptional control.
    5. skNAC binds to the E3 SUMO ligase mammalian Mms21/Nse2 and that knockdown of Nse2 expression inhibits specific aspects of myogenic differentiation, accompanied by a partial blockade of the nuclear-to-cytoplasmic translocation of the skNAC-Smyd1 complex
      Title: The E3 SUMO ligase Nse2 regulates sumoylation and nuclear-to-cytoplasmic translocation of skNAC-Smyd1 in myogenesis.
    6. depletion of alphaNAC in multiple types of cancer cells induce typical apoptotic cell death. This anti-apoptotic function is mediated by the FADD/c-Jun N-terminal kinase pathway.
      Title: αNAC inhibition of the FADD-JNK axis plays anti-apoptotic role in multiple cancer cells.
    7. We propose that maximal transcriptional repression by FIAT requires its interaction with SUMOylated, HDAC2-interacting aNAC
      Title: SUMOylated αNAC potentiates transcriptional repression by FIAT.
    8. Altered gene dosages for Galphas and alpha- NAC in compound heterozygous mice result in reduced bone mass, increased numbers of osteocytes, and enhanced expression of Sost.
      Title: The PTH-Gαs-protein kinase A cascade controls αNAC localization to regulate bone mass.
    9. The bone phenotype of compound Naca(+/-); Fiat(+/-) heterozygotes confirms that FIAT and alphaNAC are part of a common genetic pathway and support a role for the FIAT/alphaNAC interaction in normal bone physiology.
      Title: Altered gene dosage confirms the genetic interaction between FIAT and αNAC.
    10. data suggest that skNAC controls myoblast migration and sarcomere architecture in a calpain-dependent manner
      Title: skNAC depletion stimulates myoblast migration and perturbs sarcomerogenesis by enhancing calpain 1 and 3 activity.
    Submit: New GeneRIF Correction See all GeneRIFs (16)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (3)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables TBP-class protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription coactivator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables unfolded protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within myoblast migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of protein localization to endoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein targeting to membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of nascent polypeptide-associated complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    nascent polypeptide-associated complex subunit alpha
    Names
    alpha-NAC, muscle-specific form
    alpha-NAC/1.9.2
    nascent polypeptide-associated complex alpha subunit
    nascent polypeptide-associated complex subunit alpha, muscle-specific form
    NP_001106670.1
    NP_001269905.1
    NP_038636.2
    XP_036011564.1
    XP_036011565.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001113199.2 → NP_001106670.1  nascent polypeptide-associated complex subunit alpha isoform a

      See identical proteins and their annotated locations for NP_001106670.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longer isoform (a).
      Source sequence(s)
      AC131120
      Consensus CDS
      CCDS48723.1
      UniProtKB/Swiss-Prot
      E9QMB1, P70670
      Related
      ENSMUSP00000089680.6, ENSMUST00000092048.13
      Conserved Domains (3) summary
      PHA03247
      Location:1057 → 1559
      PHA03247; large tegument protein UL36; Provisional
      cd14415
      Location:2141 → 2186
      UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)
      cd22054
      Location:2056 → 2103
      NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit

    2. NM_001282976.2 → NP_001269905.1  nascent polypeptide-associated complex subunit alpha isoform b

      See identical proteins and their annotated locations for NP_001269905.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) has an additional segment in the 5' UTR and lacks an in-frame large exon in the coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. Variants 2 and 3 encode the same isoform b.
      Source sequence(s)
      AC131120
      Consensus CDS
      CCDS24258.1
      UniProtKB/Swiss-Prot
      Q3THR6, Q4FZL8, Q60817
      UniProtKB/TrEMBL
      Q3UK68
      Conserved Domains (2) summary
      cd14415
      Location:169 → 214
      UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)
      cd22054
      Location:84 → 131
      NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit

    3. NM_013608.4 → NP_038636.2  nascent polypeptide-associated complex subunit alpha isoform b

      See identical proteins and their annotated locations for NP_038636.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an internal in-frame large exon in the coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. Variants 2 and 3 encode the same isoform b.
      Source sequence(s)
      AC131120
      Consensus CDS
      CCDS24258.1
      UniProtKB/Swiss-Prot
      Q3THR6, Q4FZL8, Q60817
      UniProtKB/TrEMBL
      Q3UK68
      Related
      ENSMUSP00000073532.8, ENSMUST00000073868.9
      Conserved Domains (2) summary
      cd14415
      Location:169 → 214
      UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)
      cd22054
      Location:84 → 131
      NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000076.7 Reference GRCm39 C57BL/6J

      Range
      127869144..127884506
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036155672.1 → XP_036011565.1  nascent polypeptide-associated complex subunit alpha isoform X2

      UniProtKB/Swiss-Prot
      Q3THR6, Q4FZL8, Q60817
      UniProtKB/TrEMBL
      Q3UK68
      Conserved Domains (2) summary
      cd14415
      Location:169 → 214
      UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)
      cd22054
      Location:84 → 131
      NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit

    2. XM_036155671.1 → XP_036011564.1  nascent polypeptide-associated complex subunit alpha isoform X1

      UniProtKB/Swiss-Prot
      E9QMB1, P70670
      Conserved Domains (3) summary
      PHA03247
      Location:1057 → 1559
      PHA03247; large tegument protein UL36; Provisional
      cd14415
      Location:2141 → 2186
      UBA_NACA_NACP1; UBA-like domain found in nascent polypeptide-associated complex subunit alpha (NACA) and putative NACA-like protein (NACP1)
      cd22054
      Location:2056 → 2103
      NAC_NACA; nascent polypeptide-associated complex (NAC), alpha subunit
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P70670.2 GenPept UniProtKB/Swiss-Prot:P70670
    Q60817.1 GenPept UniProtKB/Swiss-Prot:Q60817

    Gene LinkOut

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