Pkn2 protein kinase N2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pkn2provided by MGI
Official Full Name: protein kinase N2provided by MGI
Primary source: MGI:MGI:109211
See related: Ensembl:ENSMUSG00000004591 AllianceGenome:MGI:109211
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PRK2; Stk7; Prkcl2; 6030436C20Rik
Summary: Predicted to enable RNA polymerase binding activity; histone deacetylase binding activity; and protein serine/threonine kinase activity. Involved in apical junction assembly. Located in apical junction complex; cytoplasm; and nucleus. Is expressed in several structures, including genitourinary system; heart; liver; lung; and spleen. Orthologous to human PKN2 (protein kinase N2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in placenta adult (RPKM 11.9), bladder adult (RPKM 10.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 H1; 3 66.69 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (142496663..142587765, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (142790902..142882004, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PKN2 deficiency leads both to prenatal 'congenital' cardiomyopathy and defective angiotensin II stress responses.
Title: PKN2 deficiency leads both to prenatal 'congenital' cardiomyopathy and defective angiotensin II stress responses.
Protein kinase N2 mediates flow-induced endothelial NOS activation and vascular tone regulation.
Title: Protein kinase N2 mediates flow-induced endothelial NOS activation and vascular tone regulation.
Novel roles of PRK1 and PRK2 in cilia and cancer biology.
Title: Novel roles of PRK1 and PRK2 in cilia and cancer biology.
PKN1 and PKN2 activation causes cardiac dysfunction and is involved in the transition to heart failure by phosphorylating MRTFA and disrupting its interaction with actin.
Title: Protein Kinase N Promotes Stress-Induced Cardiac Dysfunction Through Phosphorylation of Myocardin-Related Transcription Factor A and Disruption of Its Interaction With Actin.
In mature skeletal muscle, in vivo PKN2 knockdown decreased glucose uptake and increased AMPK phosphorylation. Thus, PKN2 alters key signaling pathways and transcriptional networks to regulate glucose and lipid metabolism.
Title: Protein kinase N2 regulates AMP kinase signaling and insulin responsiveness of glucose metabolism in skeletal muscle.
Mechanistically, Yersinia pseudotuberculosis YopM recruits and activates the mouse host kinases PRK1 and PRK2 to negatively regulate pyrin by phosphorylation.
Title: The Yersinia Virulence Factor YopM Hijacks Host Kinases to Inhibit Type III Effector-Triggered Activation of the Pyrin Inflammasome.
PKN2 formed complexes with Cdo, APPL1 and AKT via its C-terminal region and this interaction appeared to be important for induction of AKT activity as well as myoblast differentiation.
Title: PKN2 and Cdo interact to activate AKT and promote myoblast differentiation.
To unravel the in vivo physiological function of PKN2, we targeted the PKN2 gene. Constitutive disruption of the mouse PKN2 gene resulted in growth retardation and lethality before embryonic day (E) 10.5. PKN2(-/-) embryo did not undergo axial turning and showed insufficient closure of the neural tube.Mouse embryonic fibroblasts (MEFs) derived from PKN2(-/-) embryos at E9.5 failed to grow.
Title: PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts.
Yersinia pseudotuberculosis mutants expressing YopM proteins unable to interact with either RSK1 or PRK2 were defective for virulence in this assay, indicating that both interaction domains are important for YopM to promote pathogenesis.
Title: Delineation of regions of the Yersinia YopM protein required for interaction with the RSK1 and PRK2 host kinases and their requirement for interleukin-10 production and virulence.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D3Mit38 (e-PCR), detects polymorphism
- UniSTS:129805

NoName (e-PCR)
- UniSTS:234838

AI507382 (e-PCR)
- UniSTS:164228

BB448270 (e-PCR)
- UniSTS:207571

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase binding	ISO Inferred from Sequence Orthology more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	ISS Inferred from Sequence or Structural Similarity more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apical junction assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in apical junction assembly	ISO Inferred from Sequence Orthology more info
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cell projection organization	IEA Inferred from Electronic Annotation more info
involved_in epithelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in epithelial cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytokinesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitotic cell cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of viral genome replication	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of viral genome replication	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cell motility	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell motility	ISS Inferred from Sequence or Structural Similarity more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in apical junction complex	IDA Inferred from Direct Assay more info	PubMed
located_in apical junction complex	ISO Inferred from Sequence Orthology more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
located_in cleavage furrow	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intermediate filament cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intermediate filament cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISS Inferred from Sequence or Structural Similarity more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in midbody	ISS Inferred from Sequence or Structural Similarity more info
located_in nuclear body	IEA Inferred from Electronic Annotation more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: serine/threonine-protein kinase N2

Names: PKN gamma; protein kinase C-like 2; protein-kinase C-related kinase 2; serine/threonine kinase 7

NP_848769.2

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_178654.4 → NP_848769.2 serine/threonine-protein kinase N2

See identical proteins and their annotated locations for NP_848769.2

Status: VALIDATED

Source sequence(s)

AK049713, AK133298, AK140746, BY729073

Consensus CDS

CCDS51084.1

UniProtKB/Swiss-Prot

Q3TBR3, Q80WS2, Q8BJL7, Q8BL62, Q8BWW9

UniProtKB/TrEMBL

G3UZM9

Related

ENSMUSP00000039566.9, ENSMUST00000043812.15

Conserved Domains (6) summary

cd08687
Location:389 → 472

C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins

cd11622
Location:42 → 107

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11631
Location:128 → 201

HR1_PKN2_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N2

cd11635
Location:209 → 282

HR1_PKN2_3; Third Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N2

smart00220
Location:661 → 915

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05589
Location:656 → 981

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N