Ephx2 epoxide hydrolase 2, cytoplasmic [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ephx2provided by MGI
Official Full Name: epoxide hydrolase 2, cytoplasmicprovided by MGI
Primary source: MGI:MGI:99500
See related: Ensembl:ENSMUSG00000022040 AllianceGenome:MGI:99500
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CEH; SEH; sEP; Eph2
Summary: Enables epoxide hydrolase activity. Involved in several processes, including cholesterol homeostasis; epoxide metabolic process; and regulation of cholesterol metabolic process. Predicted to be located in cytosol. Predicted to be active in peroxisome. Is expressed in several structures, including eye; heart; integumental system; nervous system; and urinary system. Human ortholog(s) of this gene implicated in coronary artery disease; familial hypercholesterolemia; and hypertension. Orthologous to human EPHX2 (epoxide hydrolase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in liver adult (RPKM 79.4), ovary adult (RPKM 75.1) and 14 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 D1; 14 34.36 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (66321821..66361971, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (66084372..66124522, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Role of the soluble epoxide hydrolase in keratinocyte proliferation and sensitivity of skin to inflammatory stimuli.
Title: Role of the soluble epoxide hydrolase in keratinocyte proliferation and sensitivity of skin to inflammatory stimuli.
Soluble Epoxide Hydrolase Contributes to Cell Senescence and ER Stress in Aging Mice Colon.
Title: Soluble Epoxide Hydrolase Contributes to Cell Senescence and ER Stress in Aging Mice Colon.
Hepatic soluble epoxide hydrolase activity regulates cerebral Abeta metabolism and the pathogenesis of Alzheimer's disease in mice.
Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
Soluble epoxide hydrolase deficiency promotes liver regeneration and ameliorates liver injury in mice by regulating angiocrine factors and angiogenesis.
Title: Soluble epoxide hydrolase deficiency promotes liver regeneration and ameliorates liver injury in mice by regulating angiocrine factors and angiogenesis.
Targeting soluble epoxide hydrolase promotes osteogenic-angiogenic coupling via activating SLIT3/HIF-1alpha signalling pathway.
Title: Targeting soluble epoxide hydrolase promotes osteogenic-angiogenic coupling via activating SLIT3/HIF-1α signalling pathway.
Decreased Expression of Soluble Epoxide Hydrolase Suppresses Murine Choroidal Neovascularization.
Title: Decreased Expression of Soluble Epoxide Hydrolase Suppresses Murine Choroidal Neovascularization.
Role of the soluble epoxide hydrolase in the hair follicle stem cell homeostasis and hair growth.
Title: Role of the soluble epoxide hydrolase in the hair follicle stem cell homeostasis and hair growth.
Preservation of epoxyeicosatrienoic acid bioavailability prevents renal allograft dysfunction and cardiovascular alterations in kidney transplant recipients.
Title: Preservation of epoxyeicosatrienoic acid bioavailability prevents renal allograft dysfunction and cardiovascular alterations in kidney transplant recipients.
Soluble Epoxide Hydrolase Hepatic Deficiency Ameliorates Alcohol-Associated Liver Disease.
Title: Soluble Epoxide Hydrolase Hepatic Deficiency Ameliorates Alcohol-Associated Liver Disease.
sEH promotes macrophage phagocytosis and lung clearance of Streptococcus pneumoniae.
Title: sEH promotes macrophage phagocytosis and lung clearance of Streptococcus pneumoniae.

Submit: New GeneRIF Correction See all GeneRIFs (88)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (7) 1 citation
Gene trapped (2)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW106936 (e-PCR)
- UniSTS:180684

Z37107 (e-PCR)
- UniSTS:125931

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 10-hydroxy-9-(phosphonooxy)octadecanoate phosphatase activity	IEA Inferred from Electronic Annotation more info
enables epoxide hydrolase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables epoxide hydrolase activity	ISO Inferred from Sequence Orthology more info
enables lipid phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables lipid phosphatase activity	ISO Inferred from Sequence Orthology more info
enables lipid phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables lysophosphatidic acid phosphatase activity	ISO Inferred from Sequence Orthology more info
enables lysophosphatidic acid phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables magnesium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables toxic substance binding	IEA Inferred from Electronic Annotation more info
enables toxic substance binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cholesterol homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cholesterol homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in cholesterol homeostasis	ISO Inferred from Sequence Orthology more info
involved_in dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in dephosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in epoxide metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epoxide metabolic process	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phospholipid dephosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipid dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in phospholipid dephosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of blood pressure	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in prostaglandin production involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in regulation of cholesterol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cholesterol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to toxic substance	IEA Inferred from Electronic Annotation more info
involved_in stilbene catabolic process	IEA Inferred from Electronic Annotation more info
involved_in stilbene catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in peroxisome	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisome	IEA Inferred from Electronic Annotation more info
located_in peroxisome	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: bifunctional epoxide hydrolase 2

Names: cytosolic epoxide hydrolase; epoxide hydratase; epoxide hydrolase 2C; soluble epoxide hydrolase

NP_001258331.1

EC 3.1.3.76

EC 3.3.2.10

NP_001258332.1

EC 3.1.3.76

EC 3.3.2.10

NP_001258350.1

EC 3.1.3.76

EC 3.3.2.10

NP_031966.2

EC 3.1.3.76

EC 3.3.2.10

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271402.1 → NP_001258331.1 bifunctional epoxide hydrolase 2 isoform b

See identical proteins and their annotated locations for NP_001258331.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site at the 5' exon, differs in the 5' UTR, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.

Source sequence(s)

AC126272, AK002415

UniProtKB/TrEMBL

Q3UQ71

Conserved Domains (4) summary

cd01427
Location:58 → 150

HAD_like; Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others, ...

TIGR01509
Location:1 → 150

HAD-SF-IA-v3; haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED

pfam00561
Location:204 → 477

Abhydrolase_1; alpha/beta hydrolase fold

cl21494
Location:186 → 326

Abhydrolase; alpha/beta hydrolases
NM_001271403.1 → NP_001258332.1 bifunctional epoxide hydrolase 2 isoform c

See identical proteins and their annotated locations for NP_001258332.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, lacks an in-frame portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This variant is considered to be 5' complete based on evidence from PMID: 15601917. The encoded isoform (c) contains a distinct N-terminus and is shorter than isoform a.

Source sequence(s)

AC126272, AY098585

Consensus CDS

CCDS88694.1

UniProtKB/TrEMBL

Q3UQ71

Related

ENSMUSP00000153161.2, ENSMUST00000224698.2

Conserved Domains (4) summary

cd01427
Location:93 → 185

HAD_like; Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others, ...

TIGR01509
Location:49 → 185

HAD-SF-IA-v3; haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED

pfam00561
Location:239 → 512

Abhydrolase_1; alpha/beta hydrolase fold

cl21494
Location:221 → 361

Abhydrolase; alpha/beta hydrolases
NM_001271421.1 → NP_001258350.1 bifunctional epoxide hydrolase 2 isoform d

See identical proteins and their annotated locations for NP_001258350.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. This variant is considered to be 5' complete based on evidence from PMID: 17900570. The encoded isoform (d) has a shorter N-terminus than isoform a.

Source sequence(s)

AC126272, EF597241

Consensus CDS

CCDS88693.1

UniProtKB/TrEMBL

A8JYK8, Q3UQ71

Related

ENSMUSP00000152894.2, ENSMUST00000225309.2

Conserved Domains (4) summary

cd01427
Location:45 → 137

HAD_like; Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others, ...

TIGR01509
Location:1 → 137

HAD-SF-IA-v3; haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED

pfam00561
Location:191 → 464

Abhydrolase_1; alpha/beta hydrolase fold

cl21494
Location:173 → 313

Abhydrolase; alpha/beta hydrolases
NM_007940.4 → NP_031966.2 bifunctional epoxide hydrolase 2 isoform a

See identical proteins and their annotated locations for NP_031966.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (a).

Source sequence(s)

AC126272, AK142714

Consensus CDS

CCDS27218.1

UniProtKB/Swiss-Prot

P34914, Q8CGV0

UniProtKB/TrEMBL

Q3UQ71

Related

ENSMUSP00000069209.2, ENSMUST00000070515.2

Conserved Domains (2) summary

cd02603
Location:3 → 214

HAD_sEH-N_like; N-terminal lipase phosphatase domain of human soluble epoxide hydrolase, Escherichia coli YihX/HAD4 alpha-D-glucose 1-phosphate phosphatase, and related domains, may be inactive

pfam00561
Location:257 → 530

Abhydrolase_1; alpha/beta hydrolase fold