Spop speckle-type BTB/POZ protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spopprovided by MGI
Official Full Name: speckle-type BTB/POZ proteinprovided by MGI
Primary source: MGI:MGI:1343085
See related: Ensembl:ENSMUSG00000057522 AllianceGenome:MGI:1343085
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: TEF2; Pcif1
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Acts upstream of or within several processes, including glucose homeostasis; positive regulation of apoptotic process; and regulation of DNA-templated transcription. Located in nuclear speck. Is expressed in cerebral cortex ventricular layer; cortical plate; lung; metanephros; and pancreas. Human ortholog(s) of this gene implicated in colorectal cancer; hepatocellular carcinoma; and lung adenocarcinoma. Orthologous to human SPOP (speckle type BTB/POZ protein). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in cerebellum adult (RPKM 32.0), bladder adult (RPKM 30.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 D; 11 59.01 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (95304909..95384236)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (95414083..95493410)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction.
Title: Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction.
Spop ameliorates diabetic nephropathy through restraining NLRP3 inflammasome.
Title: Spop ameliorates diabetic nephropathy through restraining NLRP3 inflammasome.
CHD1 and SPOP synergistically protect prostate epithelial cells from DNA damage.
Title: CHD1 and SPOP synergistically protect prostate epithelial cells from DNA damage.
Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.
Title: Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.
SPOP is a key regulator of emergency hematopoiesis and inflammation sensing in the bone marrow.
Title: The E3 ubiquitin ligase SPOP controls resolution of systemic inflammation by triggering MYD88 degradation.
SPOP is required for embryo implantation, endometrial decidualization, and uterine health in the mouse
Title: Uterine function in the mouse requires speckle-type poz protein.
no evidence that ERG is an effector of SPOP mutation in human prostate cancer or mouse models.
Title: SPOP mutation drives prostate neoplasia without stabilizing oncogenic transcription factor ERG.
loss of Spop, but not Spopl, disrupts chondrocyte hypertrophy and osteoblast differentiation in the mouse, suggesting the requirement for Spop-mediated protein degradation in mouse skeletal development; overexpressed Spop targets both Gli3FL and Gli3R for ubiquitination and degradation and Spop is an important positive regulator of Ihh signaling and skeletal development
Title: Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling.
Results demonstrate a negative role of Spop in the level and activity of Gli3, Shh signaling and ventral spinal cord patterning.
Title: Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord.
Speckle-type pox virus and zinc finger (POZ) protein (SPOP) regulates endometrial stromal cell decidualization in mice and that hormones regulate the expression of SPOP. This study suggests that ubiquitination may be involved in embryonic implantation.
Title: SPOP Regulates Endometrial Stromal Cell Decidualization in Mice.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU048745 (e-PCR)
- UniSTS:145195

AI315626 (e-PCR)
- UniSTS:180293

G38054 (e-PCR)
- UniSTS:105042

D11Lis3 (e-PCR), detects polymorphism
- UniSTS:498525

L18224 (e-PCR)
- UniSTS:9065

MARC_9787-9788:996688611:1 (e-PCR)
- UniSTS:270001

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables molecular function inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within glucose homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of type B pancreatic cell apoptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of type B pancreatic cell apoptotic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of Cul3-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul3-RING ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear speck	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: speckle-type POZ protein

Names: HIB homolog 1; PDX-1 C-terminal-interacting factor 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001359107.1 → NP_001346036.1 speckle-type POZ protein

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses alternate splice sites in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AK132383, BF662657, BY172312

Consensus CDS

CCDS36286.1

UniProtKB/Swiss-Prot

Q3TLC2, Q5ST07, Q6ZWS8, Q76LV9

UniProtKB/TrEMBL

Q3THQ1

Related

ENSMUSP00000103350.2, ENSMUST00000107722.8

Conserved Domains (3) summary

cd03774
Location:28 → 166

MATH_SPOP; Speckle-type POZ protein (SPOP) family, MATH domain; composed of proteins with similarity to human SPOP. SPOP was isolated as a novel antigen recognized by serum from a scleroderma patient, whose overexpression in COS cells results in a discrete speckled ...

cd18279
Location:182 → 301

BTB_POZ_SPOP-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in speckle-type POZ protein (SPOP) and similar proteins

cd18518
Location:297 → 367

BACK_SPOP; BACK (BTB and C-terminal Kelch) domain found in speckle-type POZ protein (SPOP)
NM_025287.2 → NP_079563.2 speckle-type POZ protein

See identical proteins and their annotated locations for NP_079563.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

AB071989, AK004669, BY303667, BY726358, CJ207806

Consensus CDS

CCDS36286.1

UniProtKB/Swiss-Prot

Q3TLC2, Q5ST07, Q6ZWS8, Q76LV9

UniProtKB/TrEMBL

Q3THQ1

Related

ENSMUSP00000103352.3, ENSMUST00000107724.9

Conserved Domains (3) summary

cd03774
Location:28 → 166

MATH_SPOP; Speckle-type POZ protein (SPOP) family, MATH domain; composed of proteins with similarity to human SPOP. SPOP was isolated as a novel antigen recognized by serum from a scleroderma patient, whose overexpression in COS cells results in a discrete speckled ...

cd18279
Location:182 → 301

BTB_POZ_SPOP-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in speckle-type POZ protein (SPOP) and similar proteins

cd18518
Location:297 → 367

BACK_SPOP; BACK (BTB and C-terminal Kelch) domain found in speckle-type POZ protein (SPOP)