Plat plasminogen activator, tissue [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Platprovided by MGI
Official Full Name: plasminogen activator, tissueprovided by MGI
Primary source: MGI:MGI:97610
See related: Ensembl:ENSMUSG00000031538 AllianceGenome:MGI:97610
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: tPA; D8Ertd2e
Summary: This gene encodes a key enzyme of the fibrinolytic pathway. The encoded protein undergoes proteolytic processing by plasmin to generate a heterodimeric serine protease that cleaves the proenzyme plasminogen to produce plasmin, a protease that is required to break down fibrin clots. Additionally, the encoded protein is involved in other biological processes such as synaptic plasticity, cell migration and tissue remodeling. Mice lacking the encoded protein display a reduction in long-term potentiation in hippocampus and conversely, transgenic mice overexpressing the encoded protein have increased and prolonged long-term potentiation. [provided by RefSeq, Jul 2015]
Expression: Broad expression in ovary adult (RPKM 44.8), subcutaneous fat pad adult (RPKM 41.2) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 A2; 8 11.42 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (23247655..23272864)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (22757722..22782848)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Janus face of endogenous neuronal tPA: promoting self-protection and worsening the death of neighboring neurons.
Title: The Janus face of endogenous neuronal tPA: promoting self-protection and worsening the death of neighboring neurons.
Mechanical induction of osteoanabolic Wnt1 promotes osteoblast differentiation via Plat.
Title: Mechanical induction of osteoanabolic Wnt1 promotes osteoblast differentiation via Plat.
Role of tPA in Corticosterone-Induced Apoptosis of Mouse Mural Granulosa and Oviductal Epithelial Cells.
Title: Role of tPA in Corticosterone-Induced Apoptosis of Mouse Mural Granulosa and Oviductal Epithelial Cells.
Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors.
Title: Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors.
tPA Deficiency Underlies Neurovascular Coupling Dysfunction by Amyloid-beta.
Title: tPA Deficiency Underlies Neurovascular Coupling Dysfunction by Amyloid-β.
Interacting hepatic PAI-1/tPA gene regulatory pathways influence impaired fibrinolysis severity in obesity.
Title: Interacting hepatic PAI-1/tPA gene regulatory pathways influence impaired fibrinolysis severity in obesity.
This effect requires endothelial NMDAR and is t-PA-dependent. These neurodevelopmental data reinforce the debate regarding safety of molecules with NMDA-antagonist properties administered to preterm and term neonates.
Title: Glutamate controls vessel-associated migration of GABA interneurons from the pial migratory route via NMDA receptors and endothelial protease activation.
Sata suggest that tissue-type plasminogen activator (tPA) regulates nocturnal wheel-running activity, and that tPA differentially affects suprachiasmatic nucleus-driven nocturnal activity rhythms and activity driven by fasting or temporal food restriction.
Title: Regulation of Locomotor activity in fed, fasted, and food-restricted mice lacking tissue-type plasminogen activator.
Our findings suggest that isoflurane pretreatment could attenuate tPA-exaggerated brain ischemic injury, by reducing tPA-induced LRP/NF-kappaB/Cox-2 in endothelial cells, endothelial MMP-2 and MMP-9 activation, and subsequent pro-apoptotic molecule in neurons after oxygen/glucose deprivation and reperfusion.
Title: Isoflurane preconditioning inhibits the effects of tissue-type plasminogen activator on brain endothelial cell in an in vitro model of ischemic stroke.
Tissue plasminogen activator was found to be restricted to subsets of excitatory pyramidal glutamatergic neurons.
Title: Tissue Plasminogen Activator Expression Is Restricted to Subsets of Excitatory Pyramidal Glutamatergic Neurons.

Submit: New GeneRIF Correction See all GeneRIFs (124)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6) 1 citation
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW212668 (e-PCR)
- UniSTS:179712

NoName (e-PCR)
- UniSTS:224830

RH125941 (e-PCR)
- UniSTS:162653

Plat (e-PCR), detects polymorphism
- UniSTS:525931

RH136573 (e-PCR)
- UniSTS:210553

Plat (e-PCR), detects polymorphism
- UniSTS:531090

RH136350 (e-PCR)
- UniSTS:210340

RH94475 (e-PCR)
- UniSTS:119723

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables phosphoprotein binding	IEA Inferred from Electronic Annotation more info
enables phosphoprotein binding	ISO Inferred from Sequence Orthology more info
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	IEA Inferred from Electronic Annotation more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in negative regulation of fibrinolysis	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of plasminogen activation	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of proteolysis	ISO Inferred from Sequence Orthology more info
involved_in plasminogen activation	IBA Inferred from Biological aspect of Ancestor more info
involved_in plasminogen activation	IEA Inferred from Electronic Annotation more info
involved_in plasminogen activation	ISO Inferred from Sequence Orthology more info
involved_in platelet-derived growth factor receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within platelet-derived growth factor receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within platelet-derived growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in prevention of polyspermy	ISO Inferred from Sequence Orthology more info
involved_in prevention of polyspermy	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within proteolysis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in smooth muscle cell migration	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within smooth muscle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic transmission, glutamatergic	ISO Inferred from Sequence Orthology more info
involved_in trans-synaptic signaling by BDNF, modulating synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in trans-synaptic signaling by BDNF, modulating synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IEA Inferred from Electronic Annotation more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in neuronal dense core vesicle	ISO Inferred from Sequence Orthology more info
is_active_in perisynaptic space	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
part_of serine protease inhibitor complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: tissue-type plasminogen activator

Names: t-plasminogen activator

NP_032898.2

EC 3.4.21.68

XP_036009719.1

EC 3.4.21.68

XP_036009720.1

EC 3.4.21.68

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008872.3 → NP_032898.2 tissue-type plasminogen activator preproprotein

See identical proteins and their annotated locations for NP_032898.2

Status: REVIEWED

Source sequence(s)

AC121835, AK135965, CJ146601, DV644940

Consensus CDS

CCDS22183.1

UniProtKB/Swiss-Prot

P11214, Q6P7U0, Q91VP2

Related

ENSMUSP00000033941.6, ENSMUST00000033941.7

Conserved Domains (5) summary

smart00058
Location:38 → 80

FN1; Fibronectin type 1 domain

cd00108
Location:210 → 295

KR; Kringle domain; Kringle domains are believed to play a role in binding mediators, such as peptides, other proteins, membranes, or phospholipids. They are autonomous structural domains, found in a varying number of copies, in blood clotting and ...

cd00190
Location:311 → 556

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

pfam00008
Location:83 → 115

EGF; EGF-like domain

pfam00051
Location:124 → 205

Kringle; Kringle domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

23247655..23272864

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036153827.1 → XP_036009720.1 tissue-type plasminogen activator isoform X2

Conserved Domains (3) summary

smart00058
Location:38 → 80

FN1; Fibronectin type 1 domain

cd00108
Location:84 → 169

KR; Kringle domain; Kringle domains are believed to play a role in binding mediators, such as peptides, other proteins, membranes, or phospholipids. They are autonomous structural domains, found in a varying number of copies, in blood clotting and ...

cd00190
Location:185 → 430

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...
XM_036153826.1 → XP_036009719.1 tissue-type plasminogen activator isoform X1

UniProtKB/Swiss-Prot

P11214, Q6P7U0, Q91VP2

Conserved Domains (5) summary

smart00058
Location:38 → 80

FN1; Fibronectin type 1 domain

cd00108
Location:210 → 295

KR; Kringle domain; Kringle domains are believed to play a role in binding mediators, such as peptides, other proteins, membranes, or phospholipids. They are autonomous structural domains, found in a varying number of copies, in blood clotting and ...

cd00190
Location:311 → 556

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

pfam00008
Location:83 → 115

EGF; EGF-like domain

pfam00051
Location:124 → 205

Kringle; Kringle domain