Izumo1 izumo sperm-egg fusion 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Izumo1provided by MGI
Official Full Name: izumo sperm-egg fusion 1provided by MGI
Primary source: MGI:MGI:1920706
See related: Ensembl:ENSMUSG00000064158 AllianceGenome:MGI:1920706
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Izumo; 1700058F15Rik
Summary: Enables protein binding activity involved in heterotypic cell-cell adhesion; protein homodimerization activity; and signaling receptor binding activity. Involved in fusion of sperm to egg plasma membrane involved in single fertilization; heterotypic cell-cell adhesion; and sperm-egg recognition. Located in acrosomal membrane and endoplasmic reticulum membrane. Part of protein complex involved in cell-cell adhesion. Is active in plasma membrane. Orthologous to human IZUMO1 (izumo sperm-oocyte fusion 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Restricted expression toward testis adult (RPKM 34.7) See more
Orthologs: human all
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Genomic context

Location:: 7 B3; 7 29.39 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (45270251..45276653)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (45621811..45627242)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.
Title: A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.
Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice.
Title: Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice.
Collectively, this study characterizes multimeric protein complexes on the sperm surface and identifies GLIPRL1L1 as a physiologically relevant regulator of IZUMO1 function and the fertilization process.
Title: GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse.
dynamics of IZUMO1 dimerization during the acrosome reaction in spermatozoa, is reported.
Title: Monitoring dimeric status of IZUMO1 during the acrosome reaction in living spermatozoon.
IZUMO1 interacts with JUNO during sperm binding.Trp62 of JUNO interacts with hydrophobic residues on the alpha-helical core region of IZUMO1.
Title: Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization.
These data support the hypothesis that dynamic movement of IZUMO1 is essential for gamete fusion during mouse fertilization.
Title: Effects of latrunculin A on the relocation of sperm IZUMO1 during gamete interaction in mouse.
The crystal structure of Izumo1 reveals a membrane distal region composed of a four-helix bundle connected to a carboxy-terminal immunoglobulin (Ig)-like domain through a beta-hairpin stabilized by disulfide bonds. Remarkably, different regions of Izumo1 display significant structural similarities to two proteins expressed by the invasive sporozoite stage of Plasmodium parasites: SPECT1, and TRAP.
Title: The structure of sperm Izumo1 reveals unexpected similarities with Plasmodium invasion proteins.
Mice without the cytoplasmic tail of IZUMO1 showed normal fertility but decreased the amount of protein, indicating that whilst this region is important for the expression level of IZUMO1, it is dispensable for fertilisation in the mouse
Title: CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility.
Five IZUMO BCE were identified: DLVLDCL177-183, YSFYRV196-201 (named BCE-2), YLT217-219, SMVGPED221-227, and DAGNY228-232. Active immunization with the BCE-2 vaccine sharply decreased the fertility rate in female mice in a safe and reversible manner.
Title: Vaccination with an Epitope Peptide of IZUMO1 to Induce Contraception in Female Mice.
Robust IZUMO1-dependent adhesion of sperm with an oocyte accompanies the dimerization of IZUMO1.
Title: Oocyte-triggered dimerization of sperm IZUMO1 promotes sperm-egg fusion in mice.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Targeted (1) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding involved in heterotypic cell-cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding involved in heterotypic cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed
enables protein binding involved in heterotypic cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables receptor ligand activity	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in binding of sperm to zona pellucida	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell adhesion	IC Inferred by Curator more info	PubMed
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IBA Inferred from Biological aspect of Ancestor more info
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IDA Inferred from Direct Assay more info	PubMed
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	ISO Inferred from Sequence Orthology more info
involved_in heterotypic cell-cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in heterotypic cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in single fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sperm-egg recognition	IBA Inferred from Biological aspect of Ancestor more info
involved_in sperm-egg recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in syncytium formation by plasma membrane fusion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in acrosomal membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in acrosomal membrane	IDA Inferred from Direct Assay more info	PubMed
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
part_of protein complex involved in cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: izumo sperm-egg fusion protein 1

Names: OBF; oocyte binding/fusion factor; sperm-specific protein Izumo

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001018013.2 → NP_001018013.1 izumo sperm-egg fusion protein 1 isoform 1 precursor

Status: VALIDATED

Source sequence(s)

AC149057

Consensus CDS

CCDS21255.1

UniProtKB/Swiss-Prot

Q9D9J7

Related

ENSMUSP00000033100.5, ENSMUST00000033100.5

Conserved Domains (2) summary

pfam15005
Location:22 → 165

IZUMO; Izumo sperm-egg fusion, Ig domain-associated

pfam16706
Location:170 → 255

Izumo-Ig; Izumo-like Immunoglobulin domain
NM_001418984.1 → NP_001405913.1 izumo sperm-egg fusion protein 1 isoform 2

Status: VALIDATED

Source sequence(s)

AC149057

UniProtKB/TrEMBL

A0A455R725
NM_001418985.1 → NP_001405914.1 izumo sperm-egg fusion protein 1 isoform 3

Status: VALIDATED

Source sequence(s)

AC149057
NM_001418986.1 → NP_001405915.1 izumo sperm-egg fusion protein 1 isoform 1 precursor

Status: VALIDATED

Source sequence(s)

AC149057

UniProtKB/Swiss-Prot

Q9D9J7

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

45270251..45276653

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036153471.1 → XP_036009364.1 izumo sperm-egg fusion protein 1 isoform X3

Conserved Domains (2) summary

pfam15005
Location:53 → 196

IZUMO; Izumo sperm-egg fusion, Ig domain-associated

pfam16706
Location:201 → 282

Izumo-Ig; Izumo-like Immunoglobulin domain
XM_036153472.1 → XP_036009365.1 izumo sperm-egg fusion protein 1 isoform X4

Conserved Domains (2) summary

pfam15005
Location:53 → 196

IZUMO; Izumo sperm-egg fusion, Ig domain-associated

pfam16706
Location:201 → 282

Izumo-Ig; Izumo-like Immunoglobulin domain