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    Pfkfb1 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 [ Mus musculus (house mouse) ]

    Gene ID: 18639, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Pfkfb1provided by MGI
    Official Full Name
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1provided by MGI
    Primary source
    MGI:MGI:107816
    See related
    Ensembl:ENSMUSG00000025271 AllianceGenome:MGI:107816
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    PFK/FBPase 1
    Summary
    Enables fructose-2,6-bisphosphate 2-phosphatase activity. Involved in negative regulation of glycolytic process through fructose-6-phosphate. Predicted to be part of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex. Predicted to be active in cytosol. Orthologous to human PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in liver E18 (RPKM 56.0), mammary gland adult (RPKM 15.0) and 5 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Pfkfb1 in Genome Data Viewer
    Location:
    X F3; X 68.46 cM
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (149370441..149426874)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (150587445..150643878)

    Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene apurinic/apyrimidinic endonuclease 2 Neighboring gene microRNA 3620 Neighboring gene Alas2R1 erythroid cis-regulatory module Neighboring gene aminolevulinic acid synthase 2, erythroid Neighboring gene Alas2R3 erythroid cis-regulatory module Neighboring gene trophinin Neighboring gene voltage-dependent anion channel 1 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate. Van Schaftingen E, et al. Proc Natl Acad Sci U S A, 1981 May. PMID 6265919, Free PMC Article
    2. Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Properties of phospho- and dephospho- forms and of two mutants in which Ser32 has been changed by site-directed mutagenesis. Kurland IJ, et al. J Biol Chem, 1992 Mar 5. PMID 1339450
    3. Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone. Darville MI, et al. FEBS Lett, 1987 Nov 30. PMID 2856848
    4. Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice using in vivo (13)C NMR measurements of hepatic carbohydrate metabolism. Choi IY, et al. Eur J Biochem, 2002 Sep. PMID 12230553
    5. Cardiac expression of kinase-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase inhibits glycolysis, promotes hypertrophy, impairs myocyte function, and reduces insulin sensitivity. Donthi RV, et al. J Biol Chem, 2004 Nov 12. PMID 15331593

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that cardiac overexpression of kinase-deficient PFK-2 reduces cardiac glycolysis that produced negative consequences to the heart including hypertrophy, fibrosis, and reduced cardiomyocyte function.
      Title: Cardiac expression of kinase-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase inhibits glycolysis, promotes hypertrophy, impairs myocyte function, and reduces insulin sensitivity.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (2) 

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 6-phosphofructo-2-kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 6-phosphofructo-2-kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables fructose-2,6-bisphosphate 2-phosphatase activity ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    enables fructose-2,6-bisphosphate 2-phosphatase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables fructose-6-phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables fructose-6-phosphate binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables kinase binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables kinase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in animal organ regeneration IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carbohydrate phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carbohydrate phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in fructose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of glycolytic process through fructose-6-phosphate IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in negative regulation of glycolytic process through fructose-6-phosphate IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of gluconeogenesis ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in positive regulation of glycolytic process through fructose-6-phosphate ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in response to cAMP IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to glucagon IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to glucocorticoid IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to insulin IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to starvation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1
    Names
    6PF-2-K/Fru-2,6-P2ase liver isozyme
    PFK-2/FBPase-2 gene A
    NP_001361099.1
    NP_001361100.1
    NP_001361101.1
    NP_001361102.1
    NP_001361104.1
    NP_001398692.1
    NP_001398693.1
    NP_032850.1
    XP_006528819.1
    XP_036017742.1
    XP_036017743.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001374170.1 → NP_001361099.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL672150
      UniProtKB/TrEMBL
      A2AFM9
      Conserved Domains (2) summary
      pfam00300
      Location:231 → 417
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:7 → 228
      6PF2K; 6-phosphofructo-2-kinase

    2. NM_001374171.1 → NP_001361100.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL672150
      Consensus CDS
      CCDS30464.1
      UniProtKB/TrEMBL
      A2AFM9
      Conserved Domains (2) summary
      pfam00300
      Location:231 → 417
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:7 → 228
      6PF2K; 6-phosphofructo-2-kinase

    3. NM_001374172.1 → NP_001361101.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL672150
      Consensus CDS
      CCDS30464.1
      UniProtKB/TrEMBL
      A2AFM9
      Conserved Domains (2) summary
      pfam00300
      Location:231 → 417
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:7 → 228
      6PF2K; 6-phosphofructo-2-kinase

    4. NM_001374173.1 → NP_001361102.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AL672150
      Consensus CDS
      CCDS90763.1
      UniProtKB/Swiss-Prot
      A2AFN0, P70266, Q3V180
      Related
      ENSMUSP00000108333.3, ENSMUST00000112713.3
      Conserved Domains (2) summary
      pfam01591
      Location:33 → 251
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:254 → 437
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    5. NM_001374175.1 → NP_001361104.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AL672150
      UniProtKB/TrEMBL
      A2AFM8
      Conserved Domains (1) summary
      pfam01591
      Location:33 → 210
      6PF2K; 6-phosphofructo-2-kinase

    6. NM_001411763.1 → NP_001398692.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL672150
      UniProtKB/TrEMBL
      A2AFM9

    7. NM_001411764.1 → NP_001398693.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AL672150

    8. NM_008824.4 → NP_032850.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

      See identical proteins and their annotated locations for NP_032850.1

      Status: VALIDATED

      Source sequence(s)
      AL672150
      Consensus CDS
      CCDS30464.1
      UniProtKB/TrEMBL
      A2AFM9
      Related
      ENSMUSP00000079692.5, ENSMUST00000080884.11
      Conserved Domains (2) summary
      pfam00300
      Location:231 → 417
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:7 → 228
      6PF2K; 6-phosphofructo-2-kinase

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000086.8 Reference GRCm39 C57BL/6J

      Range
      149370441..149426874
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036161850.1 → XP_036017743.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X2

      UniProtKB/TrEMBL
      A2AFM9
      Conserved Domains (2) summary
      pfam00300
      Location:231 → 417
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:7 → 228
      6PF2K; 6-phosphofructo-2-kinase

    2. XM_006528756.4 → XP_006528819.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X4

      Conserved Domains (2) summary
      pfam01591
      Location:33 → 251
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:254 → 366
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    3. XM_036161849.1 → XP_036017742.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X1

      Conserved Domains (2) summary
      pfam00300
      Location:235 → 421
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:30 → 232
      6PF2K; 6-phosphofructo-2-kinase
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P70266.2 GenPept UniProtKB/Swiss-Prot:P70266

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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