RIB2 bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RIB2
Official Full Name: bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2
Primary source: SGD:S000005427
Locus tag: YOL066C
See related: AllianceGenome:SGD:S000005427; FungiDB:YOL066C; VEuPathDB:YOL066C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PUS8
Summary: Enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity and pseudouridine synthase activity. Involved in riboflavin biosynthetic process and tRNA pseudouridine synthesis. Located in cytoplasm. Orthologous to human RPUSD2 (RNA pseudouridine synthase domain containing 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RIB2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (202696..204471, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

two enzymes, Rib2/Pus8p and Pus9p, are required for Psi32 formation in cytoplasmic and mitochondrial tRNAs, respectively
Title: Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables deaminase activity	IEA Inferred from Electronic Annotation more info
enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity	IEA Inferred from Electronic Annotation more info
enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables pseudouridine synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables pseudouridine synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables pseudouridine synthase activity	IEA Inferred from Electronic Annotation more info
enables pseudouridine synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables tRNA pseudouridine(32) synthase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in enzyme-directed rRNA pseudouridine synthesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleobase-containing compound biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in pseudouridine synthesis	IEA Inferred from Electronic Annotation more info
involved_in riboflavin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in riboflavin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA processing	IEA Inferred from Electronic Annotation more info
involved_in tRNA pseudouridine synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in tRNA pseudouridine synthesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2

NP_014575.1

EC 3.5.4.26

EC 5.4.99.28

Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

202696..204471 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183321.1 → NP_014575.1 TPA: bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014575.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W201, Q12362

UniProtKB/TrEMBL

A6ZNE4, B3LIZ4, C7GK33, N1NZ56

Conserved Domains (2) summary

TIGR00005
Location:94 → 406

rluA_subfam; pseudouridine synthase, RluA family

pfam18785
Location:459 → 580

Inv-AAD; Invertebrate-AID/APOBEC-deaminase