prp-8 Pre-mRNA-splicing factor 8 homolog [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: prp-8
Official Full Name: Pre-mRNA-splicing factor 8 homolog
Primary source: WormBase:WBGene00004187
Locus tag: CELE_C50C3.6
See related: AllianceGenome:WB:WBGene00004187
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable pre-mRNA intronic binding activity and snRNA binding activity. Predicted to be involved in spliceosomal tri-snRNP complex assembly. Predicted to be located in nucleus. Predicted to be part of U5 snRNP and catalytic step 2 spliceosome. Is expressed in germ line; intestine; and pharynx. Used to study retinitis pigmentosa and retinitis pigmentosa 13. Human ortholog(s) of this gene implicated in retinitis pigmentosa and retinitis pigmentosa 13. Orthologous to human PRPF8 (pre-mRNA processing factor 8). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See prp-8 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 10

Sequence:: Chromosome: III; NC_003281.10 (8164373..8172710)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5' splice site identity.
Title: A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5' splice site identity.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C50C3.6 (e-PCR)
- UniSTS:309359

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables U1 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U2 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U5 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U5 snRNA binding	IEA Inferred from Electronic Annotation more info
enables U6 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U6 snRNA binding	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables pre-mRNA intronic binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA splicing, via spliceosome	IEA Inferred from Electronic Annotation more info
involved_in spliceosomal tri-snRNP complex assembly	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of U5 snRNP	IBA Inferred from Biological aspect of Ancestor more info
part_of catalytic step 2 spliceosome	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pre-mRNA-splicing factor 8 homolog

NP_498785.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

8164373..8172710

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_066384.7 → NP_498785.1 Pre-mRNA-splicing factor 8 homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_498785.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P34369

Conserved Domains (8) summary

cd08056
Location:2060 → 2310

MPN_PRP8; Mpr1p, Pad1p N-terminal (MPN) domains without isopeptidase activity found in splicing factor Prp8

COG5178
Location:5 → 2329

PRP8; U5 snRNP spliceosome subunit [RNA processing and modification]

cd13838
Location:1759 → 2009

RNase_H_like_Prp8_IV; Ribonuclease-like Prp8 domain IV core

pfam08082
Location:48 → 198

PRO8NT; PRO8NT (NUC069), PrP8 N-terminal domain

pfam08083
Location:388 → 788

PROCN; PROCN (NUC071) domain

pfam10596
Location:1435 → 1593

U6-snRNA_bdg; U6-snRNA interacting domain of PrP8

pfam10597
Location:1202 → 1334

U5_2-snRNA_bdg; U5-snRNA binding site 2 of PrP8

pfam10598
Location:978 → 1069

RRM_4; RNA recognition motif of the spliceosomal PrP8