eff-1 Protein kinase domain-containing protein [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: eff-1
Official Full Name: Protein kinase domain-containing protein
Primary source: WormBase:WBGene00001159
Locus tag: CELE_C26D10.5
See related: AllianceGenome:WB:WBGene00001159
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables fusogenic activity and identical protein binding activity. Involved in several processes, including egg-laying behavior; male anatomical structure morphogenesis; and nematode pharyngeal muscle development. Located in cell-cell contact zone; cytoplasm; and plasma membrane. Is expressed in several structures, including P3.pa; P3.pp; hypodermis; tail spike; and vulval cell. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See eff-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 9

Sequence:: Chromosome: II; NC_003280.10 (8346684..8350563)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A role for the fusogen eff-1 in epidermal stem cell number robustness in Caenorhabditis elegans.
Title: A role for the fusogen eff-1 in epidermal stem cell number robustness in Caenorhabditis elegans.
Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin-2 and EFF-1 to Promote Membrane Repair in C. elegans.
Title: Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin-2 and EFF-1 to Promote Membrane Repair in C. elegans.
we show that the small GTPase RAB-5 is a key cell-intrinsic regulator of the fusogen EFF-1 and can in turn regulate axonal fusion
Title: Disruption of RAB-5 Increases EFF-1 Fusogen Availability at the Cell Surface and Promotes the Regenerative Axonal Fusion Capacity of the Neuron.
EFF-1 localizes to phagocyte pseudopod tips and acts exoplasmically to drive phagosome sealing.
Title: EFF-1 fusogen promotes phagosome sealing during cell process clearance in Caenorhabditis elegans.
EFF-1 control dendrite morphology by regulating the localization pattern of SAX-7 in epidermal cells
Title: Dynein and EFF-1 control dendrite morphology by regulating the localization pattern of SAX-7 in epidermal cells.
let-7 miRNA inhibits functional restoration via EFF-1-mediated axonal self-fusion by reducing ced-7 expression.
Title: let-7 miRNA controls CED-7 homotypic adhesion and EFF-1-mediated axonal self-fusion to restore touch sensation following injury.
Endocytosis regulates membrane localization and function of the fusogen EFF-1
Title: Endocytosis regulates membrane localization and function of the fusogen EFF-1.
EFF-1 and AFF-1 fusion proteins emerge as new players in neuronal arborization and maintenance of arbor connectivity following injury in Caenorhabditis elegans Our results demonstrate that there is a genetically determined multi-step pathway to repair broken dendrites in which EFF-1 and AFF-1 act on different steps of the pathway.
Title: Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in Caenorhabditis elegans.
EFF-1 enhanced the F-actin bundling activity of VAB-10A in vitro, and actin dynamics in the cortex were reduced in eff-1 or vab-10a mutants.
Title: Spectraplakin Induces Positive Feedback between Fusogens and the Actin Cytoskeleton to Promote Cell-Cell Fusion.
RAB-5- and DYNAMIN-1-mediated endocytosis of EFF-1 fusogen controls cell-cell fusion.
Title: RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C26D10.5 (e-PCR)
- UniSTS:307276

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables fusogenic activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell-cell fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in egg-laying behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic body morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in morphogenesis of an epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nematode male tail mating organ morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nematode male tail tip morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nematode pharyngeal muscle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-embryonic body morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in syncytium formation by plasma membrane fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in syncytium formation by plasma membrane fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in syncytium formation by plasma membrane fusion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in syncytium formation by plasma membrane fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vulval development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell-cell contact zone	IBA Inferred from Biological aspect of Ancestor more info
located_in cell-cell contact zone	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Protein kinase domain-containing protein

NP_001021987.1

Confirmed by transcript evidence

NP_001021988.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

8346684..8350563

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001026817.5 → NP_001021988.1 Protein kinase domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001021988.1

Status: REVIEWED

UniProtKB/TrEMBL

G5EBM0

Conserved Domains (1) summary

pfam14884
Location:16 → 583

EFF-AFF; Type I membrane glycoproteins cell-cell fusogen
NM_001026816.7 → NP_001021987.1 Protein kinase domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001021987.1

Status: REVIEWED

UniProtKB/TrEMBL

G5ECA1

Conserved Domains (1) summary

pfam14884
Location:68 → 547

EFF-AFF; Type I membrane glycoproteins cell-cell fusogen