U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    fleQ transcriptional regulator FleQ [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 881960, updated on 11-Sep-2024

    Summary

    Go to the top of the page Help
    Gene symbol
    fleQ
    Gene description
    transcriptional regulator FleQ
    Locus tag
    PA1097
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_002516.2 (1187587..1189059)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene B-type flagellar protein FliS Neighboring gene hypothetical protein Neighboring gene two-component sensor Neighboring gene two-component response regulator

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Sensor I Regulated ATPase Activity of FleQ Is Essential for Motility to Biofilm Transition in Pseudomonas aeruginosa. Banerjee P, et al. ACS Chem Biol, 2019 Jul 19. PMID 31268665
    2. The REC domain mediated dimerization is critical for FleQ from Pseudomonas aeruginosa to function as a c-di-GMP receptor and flagella gene regulator. Su T, et al. J Struct Biol, 2015 Oct. PMID 26362077
    3. A conservative amino acid mutation in the master regulator FleQ renders Pseudomonas aeruginosa aflagellate. Jain R, et al. PLoS One, 2014. PMID 24827992, Free PMC Article
    4. Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ. Baraquet C, et al. Proc Natl Acad Sci U S A, 2013 Nov 12. PMID 24167275, Free PMC Article
    5. FleQ, the major flagellar gene regulator in Pseudomonas aeruginosa, binds to enhancer sites located either upstream or atypically downstream of the RpoN binding site. Jyot J, et al. J Bacteriol, 2002 Oct. PMID 12218010, Free PMC Article

    See all (21) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. This study determined the crystal structures of the ATPase domain of wild-type FleQ and sensor I mutants H287N and H287A in complex with ATPgammaS and Mg(2+) to 2.4, 1.95, and 2.25 A resolution, respectively.
      Title: Sensor I Regulated ATPase Activity of FleQ Is Essential for Motility to Biofilm Transition in Pseudomonas aeruginosa.
    2. FleQ mutation prevented flagellar assembly and reduced internalization of Pseudomonas aeruginosa independently of ExoS.
      Title: The Impact of ExoS on Pseudomonas aeruginosa Internalization by Epithelial Cells Is Independent of fleQ and Correlates with Bistability of Type Three Secretion System Gene Expression.
    3. The dimer is the functional form of FleN, and it is nucleotide binding and not hydrolysis by FleN that is necessary to exert an antagonistic effect against FleQ.
      Title: ATP-Induced Structural Remodeling in the Antiactivator FleN Enables Formation of the Functional Dimeric Form.
    4. The atypical REC domain of FleQ is essential for the function of FleQ.
      Title: The REC domain mediated dimerization is critical for FleQ from Pseudomonas aeruginosa to function as a c-di-GMP receptor and flagella gene regulator.
    5. The ability of FleQ to positively regulate flagellar gene expression.
      Title: A conservative amino acid mutation in the master regulator FleQ renders Pseudomonas aeruginosa aflagellate.
    6. Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ.
      Title: Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ.
    7. Analysis of wild type and mutated versions of pelA-lacZ transcriptional fusions suggests that FleQ represses gene expression from box 2 and activates gene expression in response to c-di-GMP from box 1.
      Title: The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP.
    8. The results show that FleQ is a new type of cyclic diguanylate binding protein that controls the transcriptional regulation of EPS biosynthesis genes in P. aeruginosa.
      Title: Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor.
    Submit: New GeneRIF Correction

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by PseudoCAP

    Function Evidence Code Pubs
    enables DNA-binding transcription activator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription repressor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cyclic-di-GMP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cyclic-di-GMP binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of extracellular matrix assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of cell-substrate adhesion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of cilium-dependent cell motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of bacterial-type flagellum-dependent cell motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    transcriptional regulator FleQ
    NP_249788.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      1187587..1189059
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_249788.1 transcriptional regulator FleQ [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_249788.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      Q51452
      Conserved Domains (1) summary
      COG2204
      Location:1 → 480
      AtoC; DNA-binding transcriptional response regulator, NtrC family, contains REC, AAA-type ATPase, and a Fis-type DNA-binding domains [Signal transduction mechanisms]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    G3XCV0.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials