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    wzx O-antigen translocase [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 882590, updated on 11-Sep-2024

    Summary

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    Gene symbol
    wzx
    Gene description
    O-antigen translocase
    Locus tag
    PA3153
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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    Genomic context

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    Sequence:
    NC_002516.2 (3536576..3537811, complement)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene LPS biosynthesis protein WbpG Neighboring gene imidazole glycerol phosphate synthase subunit HisF Neighboring gene imidazole glycerol phosphate synthase subunit HisH Neighboring gene B-band O-antigen polymerase Neighboring gene UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase Neighboring gene UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase Neighboring gene acetyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Proton-dependent gating and proton uptake by Wzx support O-antigen-subunit antiport across the bacterial inner membrane. Islam ST, et al. mBio, 2013 Sep 10. PMID 24023388, Free PMC Article
    2. A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO1. Islam ST, et al. Mol Microbiol, 2012 Jun. PMID 22554073, Free PMC Article
    3. Effect of wzx (rfbX) mutations on A-band and B-band lipopolysaccharide biosynthesis in Pseudomonas aeruginosa O5. Burrows LL, et al. J Bacteriol, 1999 Feb. PMID 9922263, Free PMC Article
    4. Membrane topology mapping of the O-antigen flippase (Wzx), polymerase (Wzy), and ligase (WaaL) from Pseudomonas aeruginosa PAO1 reveals novel domain architectures. Islam ST, et al. mBio, 2010 Aug 24. PMID 20824106, Free PMC Article
    5. An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase. Liu D, et al. J Bacteriol, 1996 Apr. PMID 8606190, Free PMC Article

    See all (15) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mutation of the acidic residues (E61, D269, and D359)in Wzx reduced proton modified anion flux.
      Title: Proton-dependent gating and proton uptake by Wzx support O-antigen-subunit antiport across the bacterial inner membrane.
    2. This is the first report to describe a charged flippase lumen for mediating anionic O-unit translocation across the hydrophobic inner membrane.
      Title: A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO1.
    3. The N and C termini of the O-Ag flippase were revealed to be localized in the cytoplasm, with 12 transmembrane domains.
      Title: Membrane topology mapping of the O-antigen flippase (Wzx), polymerase (Wzy), and ligase (WaaL) from Pseudomonas aeruginosa PAO1 reveals novel domain architectures.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by PseudoCAP

    Function Evidence Code Pubs
    enables antiporter activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within Gram-negative-bacterium-type cell outer membrane assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within O antigen biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within glycolipid translocation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within polysaccharide transport ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    acts_upstream_of_or_within transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Gram-negative-bacterium-type cell wall IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    O-antigen translocase
    NP_251843.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      3536576..3537811 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_251843.1 O-antigen translocase [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_251843.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      G3XD19, P72137
      Conserved Domains (1) summary
      COG2244
      Location:1 → 403
      RfbX; Membrane protein involved in the export of O-antigen and teichoic acid [Cell wall/membrane/envelope biogenesis]
    Nucleotide Protein
    Heading Accession and Version

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