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    hasAp heme acquisition protein HasA [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 879854, updated on 11-Sep-2024

    Summary

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    Gene symbol
    hasAp
    Gene description
    heme acquisition protein HasA
    Locus tag
    PA3407
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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    Genomic context

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    Sequence:
    NC_002516.2 (3813958..3814575, complement)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene hypothetical protein Neighboring gene hypothetical protein Neighboring gene metalloprotease secretion protein Neighboring gene transporter HasD Neighboring gene heme uptake outer membrane receptor HasR Neighboring gene transmembrane sensor

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Heme protects Pseudomonas aeruginosa and Staphylococcus aureus from calprotectin-induced iron starvation. Zygiel EM, et al. J Biol Chem, 2021 Jan-Jun. PMID 33273016, Free PMC Article
    2. Replacing Arginine 33 for Alanine in the Hemophore HasA from Pseudomonas aeruginosa Causes Closure of the H32 Loop in the Apo-Protein. Kumar R, et al. Biochemistry, 2016 May 10. PMID 27074415, Free PMC Article
    3. Kinetic and spectroscopic studies of hemin acquisition in the hemophore HasAp from Pseudomonas aeruginosa. Yukl ET, et al. Biochemistry, 2010 Aug 10. PMID 20586423, Free PMC Article
    4. Structural characterization of the hemophore HasAp from Pseudomonas aeruginosa: NMR spectroscopy reveals protein-protein interactions between Holo-HasAp and hemoglobin. Alontaga AY, et al. Biochemistry, 2009 Jan 13. PMID 19072037, Free PMC Article
    5. Pseudomonas Genome Database: facilitating user-friendly, comprehensive comparisons of microbial genomes. Winsor GL, et al. Nucleic Acids Res, 2009 Jan. PMID 18978025, Free PMC Article

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Heme protects Pseudomonas aeruginosa and Staphylococcus aureus from calprotectin-induced iron starvation.
      Title: Heme protects Pseudomonas aeruginosa and Staphylococcus aureus from calprotectin-induced iron starvation.
    2. stopped-flow absorption and resonance Raman analyses of hemin binding to apo-R33A HasAp indicate that the closed H32 loop slows down the insertion of the heme inside the binding pocket, presumably as it obstructs access to the hydrophobic platform on the Y75 loop, but accelerates the completion of the heme iron coordination.
      Title: Replacing Arginine 33 for Alanine in the Hemophore HasA from Pseudomonas aeruginosa Causes Closure of the H32 Loop in the Apo-Protein.
    3. kinetics and mechanism of heme acquisition by HasA; heme is initially bound by the Tyr75 to form a high-spin heme-protein complex before slower coordination of the His32 ligand upon closing of the His loop over the heme.
      Title: Kinetic and spectroscopic studies of hemin acquisition in the hemophore HasAp from Pseudomonas aeruginosa.
    4. These studies allowed identification of a specific area on the surface of truncated HasAp, encompassing the axial ligand His32 loop that serves as a transient site of interaction with hemoglobin.
      Title: Structural characterization of the hemophore HasAp from Pseudomonas aeruginosa: NMR spectroscopy reveals protein-protein interactions between Holo-HasAp and hemoglobin.
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    General gene information

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    Homology

    General protein information

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    Preferred Names
    heme acquisition protein HasA
    NP_252097.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      3813958..3814575 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_252097.1 heme acquisition protein HasA [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_252097.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      G3XD33, O69756
      Conserved Domains (1) summary
      pfam06438
      Location:1 → 177
      HasA; Heme-binding protein A (HasA)
    Nucleotide Protein
    Heading Accession and Version

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