Tent2 terminal nucleotidyltransferase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tent2provided by MGI
Official Full Name: terminal nucleotidyltransferase 2provided by MGI
Primary source: MGI:MGI:2140950
See related: Ensembl:ENSMUSG00000042167 AllianceGenome:MGI:2140950
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gld2; GLD-2; Papd4; 8030446C20Rik
Summary: Enables poly(A) RNA polymerase activity. Involved in target-directed miRNA degradation. Acts upstream of or within hematopoietic progenitor cell differentiation. Located in nucleus. Is expressed in embryo. Orthologous to human TENT2 (terminal nucleotidyltransferase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in limb E14.5 (RPKM 18.2), placenta adult (RPKM 16.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 C3; 13 47.81 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (93282363..93330662, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (93145855..93192435, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Noncanonical cytoplasmic poly(A) polymerases regulate RNA levels, alternative RNA processing, and synaptic plasticity but not hippocampal-dependent behaviours.
Title: Noncanonical cytoplasmic poly(A) polymerases regulate RNA levels, alternative RNA processing, and synaptic plasticity but not hippocampal-dependent behaviours.
In Gld2 knockout mice there are detectable alterations in specific miRNA monoadenylation in the hippocampus, but no detectable effect on miRNA stability and no overt change in animal behavior.
Title: Gld2-catalyzed 3' monoadenylation of miRNAs in the hippocampus has no detectable effect on their stability or on animal behavior.
poly(A) polymerase Gld2, deadenylase PARN, and translation inhibitory factor neuroguidin (Ngd) are components of a dendritic CPEB-associated polyadenylation apparatus
Title: Bidirectional control of mRNA translation and synaptic plasticity by the cytoplasmic polyadenylation complex.
We found an association of mGLD-2 with cytoplasmic polyadenylation components, CPEB and CPSF described in Xenopus oocytes
Title: Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
mGLD-2 may act in the ooplasm on the progression of metaphase I to metaphase II during oocyte maturation.
Title: Possible role of mouse poly(A) polymerase mGLD-2 during oocyte maturation.
identified GLD-2, which is a regulatory cytoplasmic poly(A) polymerase, as responsible for the 3'-terminal adenylation of miR-122 after unwinding of the miR-122/miR-122* duplex
Title: Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI465335 (e-PCR)
- UniSTS:180575

AI649009 (e-PCR)
- UniSTS:164285

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables poly(A) RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables poly(A) RNA polymerase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables poly(A) RNA polymerase activity	ISO Inferred from Sequence Orthology more info
enables poly(A) RNA polymerase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA 3'-end processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA polyadenylation at postsynapse	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within hematopoietic progenitor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in histone mRNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in histone mRNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in mRNA 3'-end processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA 3'-end processing	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of miRNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of miRNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of protein targeting to membrane	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in regulation of translation at postsynapse	ISO Inferred from Sequence Orthology more info
involved_in target-directed miRNA degradation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in perforant pathway to dendrate granule cell synapse	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: poly(A) RNA polymerase GLD2

Names: PAP associated domain containing 4; PAP-associated domain-containing protein 4; cytoplasmic poly(A) polymerase

NP_001348465.1

EC 2.7.7.19

NP_001348466.1

EC 2.7.7.19

NP_598666.1

EC 2.7.7.19

XP_006517573.1

EC 2.7.7.19

XP_030102937.1

EC 2.7.7.19

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001361536.1 → NP_001348465.1 poly(A) RNA polymerase GLD2 isoform 2

Status: VALIDATED

Source sequence(s)

AC120347, AC121991, AC130217

Consensus CDS

CCDS88498.1

Related

ENSMUSP00000153703.2, ENSMUST00000225868.2

Conserved Domains (1) summary

COG5260
Location:121 → 464

TRF4; DNA polymerase sigma [Replication, recombination and repair]
NM_001361537.1 → NP_001348466.1 poly(A) RNA polymerase GLD2 isoform 1

Status: VALIDATED

Source sequence(s)

AC120347, AC121991, AC130217

Consensus CDS

CCDS26686.1

UniProtKB/Swiss-Prot

A4F5H0, Q8CCH5, Q91YI6

Conserved Domains (1) summary

COG5260
Location:121 → 468

TRF4; DNA polymerase sigma [Replication, recombination and repair]
NM_133905.3 → NP_598666.1 poly(A) RNA polymerase GLD2 isoform 1

See identical proteins and their annotated locations for NP_598666.1

Status: VALIDATED

Source sequence(s)

AC120347, AC121991, AC130217

Consensus CDS

CCDS26686.1

UniProtKB/Swiss-Prot

A4F5H0, Q8CCH5, Q91YI6

Related

ENSMUSP00000048124.6, ENSMUST00000048702.7

Conserved Domains (1) summary

COG5260
Location:121 → 468

TRF4; DNA polymerase sigma [Replication, recombination and repair]

RNA

NR_154997.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC120347, AC121991, AC130217

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

93282363..93330662 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030247077.2 → XP_030102937.1 poly(A) RNA polymerase GLD2 isoform X2

Conserved Domains (1) summary

COG5260
Location:121 → 464

TRF4; DNA polymerase sigma [Replication, recombination and repair]
XM_006517510.5 → XP_006517573.1 poly(A) RNA polymerase GLD2 isoform X1

See identical proteins and their annotated locations for XP_006517573.1

UniProtKB/Swiss-Prot

A4F5H0, Q8CCH5, Q91YI6

Conserved Domains (1) summary

COG5260
Location:121 → 468

TRF4; DNA polymerase sigma [Replication, recombination and repair]