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    SCEI intron-encoded endonuclease I-SceI [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854590, updated on 9-Dec-2024

    Summary

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    Official Symbol
    SCEI
    Official Full Name
    intron-encoded endonuclease I-SceI
    Primary source
    SGD:S000007279
    Locus tag
    Q0160
    See related
    AllianceGenome:SGD:S000007279; FungiDB:Q0160; VEuPathDB:Q0160
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C, substrain: FY1679)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables endonuclease activity. Predicted to be involved in intron homing. Predicted to be located in mitochondrion. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See SCEI in Genome Data Viewer
    Sequence:
    Chromosome: MT; NC_001224.1 (61022..61729)

    Chromosome MT - NC_001224.1Genomic Context describing neighboring genes Neighboring gene tRNA-Ser Neighboring gene mitochondrial 37S ribosomal protein VAR1 Neighboring gene 21S ribosomal RNA Neighboring gene tRNA-Thr Neighboring gene tRNA-Cys

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001224.1

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Transposition of an intron in yeast mitochondria requires a protein encoded by that intron. Macreadie IG, et al. Cell, 1985 Jun. PMID 3886164
    2. Nested chromosomal fragmentation in yeast using the meganuclease I-Sce I: a new method for physical mapping of eukaryotic genomes. Thierry A, et al. Nucleic Acids Res, 1992 Nov 11. PMID 1333585, Free PMC Article
    3. Amino acid residues in both the protein splicing and endonuclease domains of the PI-SceI intein mediate DNA binding. He Z, et al. J Biol Chem, 1998 Feb 20. PMID 9468518
    4. Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron. Monteilhet C, et al. Nucleic Acids Res, 1990 Mar 25. PMID 2183191, Free PMC Article
    5. Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. Rouet P, et al. Proc Natl Acad Sci U S A, 1994 Jun 21. PMID 8016116, Free PMC Article

    See all (47) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A metal binding site is present in the active site of I-SceI that cleaves the top strand DNA.
      Title: Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
    2. findings suggest that the HSP70-subunit shields Endo.SceI from heat inactivation through ATP/ADP binding
      Title: Roles of the HSP70-subunit in a eukaryotic multi-site-specific endonuclease, Endo.SceI: autophosphorylation and heat stability.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intron homing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intron homing TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion TAS
    Traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    intron-encoded endonuclease I-SceI
    NP_009324.1
    • I-SceI DNA endonuclease; encoded by the mitochondrial group I intron of the 21S_rRNA gene; mediates gene conversion that propagates the intron into intron-less copies of the 21S_rRNA gene

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001224.1 Reference assembly

      Range
      61022..61729
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_009324.1 intron-encoded endonuclease I-SceI (mitochondrion) [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009324.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      A0A0A7NYJ1, P03882
      UniProtKB/TrEMBL
      A0A0H3WJ15, A7LCP1, E9P9U6
      Conserved Domains (1) summary
      pfam03161
      Location:34 → 208
      LAGLIDADG_2; LAGLIDADG DNA endonuclease family
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P03882.1

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