STE20 mitogen-activated protein kinase kinase kinase kinase STE20 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STE20
Official Full Name: mitogen-activated protein kinase kinase kinase kinase STE20
Primary source: SGD:S000000999
Locus tag: YHL007C
See related: AllianceGenome:SGD:S000000999; FungiDB:YHL007C; VEuPathDB:YHL007C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables MAP kinase kinase kinase kinase activity and histone H2BS14 kinase activity. Involved in several processes, including bipolar cellular bud site selection; intracellular signal transduction; and negative regulation of macromolecule biosynthetic process. Located in several cellular components, including incipient cellular bud site; mating projection tip; and nucleus. Human ortholog(s) of this gene implicated in breast cancer; ductal carcinoma in situ; non-syndromic X-linked intellectual disability 30; renal cell carcinoma; and schizophrenia. Orthologous to human PAK1 (p21 (RAC1) activated kinase 1); PAK2 (p21 (RAC1) activated kinase 2); and PAK3 (p21 (RAC1) activated kinase 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See STE20 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (95118..97937, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A Protein-Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20.
Title: A Protein-Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20.
Yeast daughter cells can polarize Cdc42 before CDK activation at start. This polarization operates via a positive feedback loop mediated by the Cdc42 effector Ste20.
Title: Cell-cycle control of cell polarity in yeast.
during filamentous growth, Ste20 stimulates V-ATPase activity, this would sustain nutrient mobilization from vacuolar stores, which is beneficial for filamentous growth.
Title: Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae.
cell cycle kinases Ste20 as central mediators of the cellular response to inhibition of fatty acid synthesis. (Ste20)
Title: Global mapping of protein phosphorylation events identifies Ste20, Sch9 and the cell-cycle regulatory kinases Cdc28/Pho85 as mediators of fatty acid starvation responses in Saccharomyces cerevisiae.
Nbp2p SH3 domain made contacts with the Ste20 peptide core RXXPXXP motif and the Bem1p SH3b domain made stronger contacts with residues flanking the core motif.
Title: Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface.
The signaling proteins Ste5 and Ste20 have recognition sequences, or "docking" sites, for G1/S (interphase) cyclins. These docking sites bind preferentially to the cyclin Cln2, and strongly enhance Cln2-driven in vivo substrate phosphorylation.
Title: Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes.
Ste20 has an unexpected role in the modulation of mRNA decay and translation in Saccharomyces cerevisiae. Phosphorylation of Dcp2 by Ste20 is an important control point for mRNA decapping.
Title: Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiae.
analysis of the solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42
Title: Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42.
An in silico predictor was built to identify Ste20p substrates on a proteome-wide basis.
Title: A biochemical genomics screen for substrates of Ste20p kinase enables the in silico prediction of novel substrates.
Results suggest that the Cdc42 effectors Ste20, Cla4, and Skm1 translocate into the nucleus, where they modulate expression of sterol uptake genes via Sut1, thereby controlling sterol homeostasis.
Title: The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase kinase kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in bipolar cellular bud site selection	IGI Inferred from Genetic Interaction more info	PubMed
involved_in bipolar cellular bud site selection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in budding cell apical bud growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in budding cell apical bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular bud site selection	HMP more info	PubMed
involved_in cellular response to hydrogen peroxide	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in invasive growth in response to glucose limitation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of sterol import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in osmosensory signaling pathway via Sho1 osmosensor	IGI Inferred from Genetic Interaction more info	PubMed
involved_in osmosensory signaling pathway via Sho1 osmosensor	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone	IEA Inferred from Electronic Annotation more info
involved_in response to pheromone triggering conjugation with cellular fusion	IPI Inferred from Physical Interaction more info	PubMed
involved_in signal transduction involved in filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sterol import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stress granule assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in stress granule assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole inheritance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vacuole inheritance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: mitogen-activated protein kinase kinase kinase kinase STE20

NP_011856.1

EC 2.7.11.1

Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

95118..97937 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179087.1 → NP_011856.1 TPA: mitogen-activated protein kinase kinase kinase kinase STE20 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011856.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKQ8, Q03497

UniProtKB/TrEMBL

G2WF60

Conserved Domains (2) summary

cd06614
Location:619 → 872

STKc_PAK; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase

pfam00786
Location:336 → 394

PBD; P21-Rho-binding domain