Dgke diacylglycerol kinase, epsilon [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgkeprovided by MGI
Official Full Name: diacylglycerol kinase, epsilonprovided by MGI
Primary source: MGI:MGI:1889276
See related: Ensembl:ENSMUSG00000000276 AllianceGenome:MGI:1889276
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DGK; DAGK6; DGK-epsilon
Summary: Predicted to enable ATP-dependent diacylglycerol kinase activity. Involved in modulation of chemical synaptic transmission and phosphatidylinositol biosynthetic process. Is active in glutamatergic synapse. Human ortholog(s) of this gene implicated in nephrotic syndrome type 7. Orthologous to human DGKE (diacylglycerol kinase epsilon). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in thymus adult (RPKM 19.4), cerebellum adult (RPKM 8.7) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 C; 11 54.34 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (88926005..88951644, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (89035179..89060795, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ablation of diacylglycerol kinase epsilon promotes whitening of brown adipose tissue under high fat diet feeding.
Title: Ablation of diacylglycerol kinase ε promotes whitening of brown adipose tissue under high fat diet feeding.
Diacylglycerol kinase-epsilon is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment.
Title: Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment.
An intact zinc finger motif of the C1B domain is critical for stability and activity of diacylglycerol kinase-epsilon.
Title: An intact zinc finger motif of the C1B domain is critical for stability and activity of diacylglycerol kinase-ε.
Diacylglycerol kinase epsilon protects against renal ischemia/reperfusion injury in mice through Kruppel-like factor 15/klotho pathway.
Title: Diacylglycerol kinase epsilon protects against renal ischemia/reperfusion injury in mice through Krüppel-like factor 15/klotho pathway.
Loss of diacylglycerol kinase epsilon causes thrombotic microangiopathy by impairing endothelial VEGFA signaling.
Title: Loss of diacylglycerol kinase ε causes thrombotic microangiopathy by impairing endothelial VEGFA signaling.
Diacylglycerol kinase zeta promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse.
Title: Diacylglycerol kinase ζ promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse.
Mice lacking DGKepsilon show increased beige adipogenesis in visceral white adipose tissue after long-term high fat diet in a COX-2- dependent manner.
Title: Mice lacking DGKε show increased beige adipogenesis in visceral white adipose tissue after long-term high fat diet in a COX-2- dependent manner.
DGKepsilon plays a role in glucose and energy homeostasis by modulating lipid metabolism in skeletal muscle
Title: Diacylglycerol kinase ε deficiency preserves glucose tolerance and modulates lipid metabolism in obese mice.
Our results unveil an unexpected role of Dgke in the induction of cyclooxygenase-2 and in the regulation of glomerular prostanoids synthesis under stress
Title: Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production.
Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity.
Title: Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C87606 (e-PCR)
- UniSTS:208729

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

Dgke (e-PCR), detects polymorphism
- UniSTS:546733

PMC31904P1 (e-PCR)
- UniSTS:273096

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables ATP-dependent diacylglycerol kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in glycerolipid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in lipid phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidic acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: diacylglycerol kinase epsilon

Names: DAG kinase epsilon; diglyceride kinase epsilon

NP_001349774.1

EC 2.7.1.107

NP_062378.1

EC 2.7.1.107

XP_011247453.1

EC 2.7.1.107

XP_036012756.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001362845.1 → NP_001349774.1 diacylglycerol kinase epsilon isoform 2

Status: VALIDATED

Source sequence(s)

AL646096

Conserved Domains (4) summary

smart00046
Location:218 → 346

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:122 → 166

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam00130
Location:58 → 107

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

cl02440
Location:366 → 505

DAGK_acc; Diacylglycerol kinase accessory domain
NM_019505.4 → NP_062378.1 diacylglycerol kinase epsilon isoform 1

See identical proteins and their annotated locations for NP_062378.1

Status: VALIDATED

Source sequence(s)

AK049335, AK147841, AL646096, BB635763

Consensus CDS

CCDS25234.1

UniProtKB/Swiss-Prot

Q5SU69, Q9R1C6

Related

ENSMUSP00000000285.3, ENSMUST00000000285.9

Conserved Domains (5) summary

TIGR00147
Location:216 → 526

TIGR00147; lipid kinase, YegS/Rv2252/BmrU family

smart00045
Location:366 → 521

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:218 → 346

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:122 → 166

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam00130
Location:58 → 107

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RNA

NR_156181.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AK147841, AL646096

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

88926005..88951644 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011249151.4 → XP_011247453.1 diacylglycerol kinase epsilon isoform X2

UniProtKB/TrEMBL

Q8C8R9

Conserved Domains (4) summary

smart00046
Location:218 → 346

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:122 → 166

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam00130
Location:58 → 105

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

cl02440
Location:366 → 505

DAGK_acc; Diacylglycerol kinase accessory domain
XM_036156863.1 → XP_036012756.1 diacylglycerol kinase epsilon isoform X1

Conserved Domains (4) summary

smart00045
Location:326 → 481

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:206 → 306

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20801
Location:55 → 108

C1_DGKepsilon_typeIII_rpt1; first protein kinase C conserved region 1 (C1 domain) found in type III diacylglycerol kinase, DAG kinase epsilon, and similar proteins

cd20853
Location:122 → 186

C1_DGKepsilon_typeIII_rpt2; second protein kinase C conserved region 1 (C1 domain) found in type III diacylglycerol kinase, DAG kinase epsilon, and similar proteins