Dnajb6 DnaJ heat shock protein family (Hsp40) member B6 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dnajb6provided by MGI
Official Full Name: DnaJ heat shock protein family (Hsp40) member B6provided by MGI
Primary source: MGI:MGI:1344381
See related: Ensembl:ENSMUSG00000029131 AllianceGenome:MGI:1344381
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mrj; mDj4; HSJ-2
Summary: Enables DNA binding activity. Acts upstream of or within several processes, including chorion development; labyrinthine layer development; and negative regulation of DNA-templated transcription. Located in cytoplasm and nucleus. Is expressed in several structures, including extraembryonic component; hemolymphoid system; nervous system; reproductive system; and sensory organ. Human ortholog(s) of this gene implicated in autosomal dominant limb-girdle muscular dystrophy type 1. Orthologous to human DNAJB6 (DnaJ heat shock protein family (Hsp40) member B6). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in testis adult (RPKM 42.9), CNS E18 (RPKM 23.9) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B1; 5 15.27 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (29940896..29991476)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (29735898..29786478)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy.
Title: A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy.
Long non-coding RNA Hsp4 alleviates lipopolysaccharide-induced apoptosis of lung epithelial cells via miRNA-466m-3p/DNAjb6 axis.
Title: Long non-coding RNA Hsp4 alleviates lipopolysaccharide-induced apoptosis of lung epithelial cells via miRNA-466m-3p/DNAjb6 axis.
Inhibition of DNAJ-HSP70 interaction improves strength in muscular dystrophy.
Title: Inhibition of DNAJ-HSP70 interaction improves strength in muscular dystrophy.
Mrj(-/-) chorions at embryonic day 8.5 have expanded Rhox4b expression domains and do not form normal layers of gene expression suggesting that chorion patterning requires Mrj.
Title: Cell-cell adhesion defects in Mrj mutant trophoblast cells are associated with failure to pattern the chorion during early placental development.
Data show that Mrj(-/-) embryos exhibit neural tube defects independent of the placenta phenotype, including exencephaly and thin-walled neural tubes, and suggest that Mrj plays an important role in neural stem cell self-renewal.
Title: Neural stem cell self-renewal requires the Mrj co-chaperone.
DnaJB6 is necessary for translocation of Slfn1 into the nucleus, where Slfn1 down-regulates cyclin D1, induces cell-cycle arrest and programmes a quiescent state of T-cells
Title: The Hsp40 family chaperone protein DnaJB6 enhances Schlafen1 nuclear localization which is critical for promotion of cell-cycle arrest in T-cells.
Study demonstrates here that, in the developing placenta of the mouse, the absence of the Mrj co-chaperone prevents proteasome degradation of keratin 18 intermediate filaments, resulting in the formation of keratin inclusion bodies.
Title: The Mrj co-chaperone mediates keratin turnover and prevents the formation of toxic inclusion bodies in trophoblast cells of the placenta.
NFATc3 is negatively regulated by class II histone deacetylases through the DnaJ (heat shock protein-40) superfamily member Mrj
Title: The DnaJ-related factor Mrj interacts with nuclear factor of activated T cells c3 and mediates transcriptional repression through class II histone deacetylase recruitment.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1) 1 citation
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126530 (e-PCR)
- UniSTS:209670

RH124732 (e-PCR)
- UniSTS:161554

NoName (e-PCR)
- UniSTS:235151

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATPase activator activity	IEA Inferred from Electronic Annotation more info
enables ATPase activator activity	ISO Inferred from Sequence Orthology more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables Hsp70 protein binding	IEA Inferred from Electronic Annotation more info
enables heat shock protein binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein folding chaperone	IBA Inferred from Biological aspect of Ancestor more info
enables protein folding chaperone	ISO Inferred from Sequence Orthology more info
enables protein-folding chaperone binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein-folding chaperone binding	ISO Inferred from Sequence Orthology more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IEA Inferred from Electronic Annotation more info
enables unfolded protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chaperone-mediated protein folding	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within chaperone-mediated protein folding	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chorio-allantoic fusion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chorion development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within extracellular matrix organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intermediate filament organization	IEA Inferred from Electronic Annotation more info
involved_in intermediate filament organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of inclusion body assembly	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inclusion body assembly	ISO Inferred from Sequence Orthology more info
involved_in protein folding	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein localization to nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within syncytiotrophoblast cell differentiation involved in labyrinthine layer development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Z disc	IEA Inferred from Electronic Annotation more info
located_in Z disc	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dnaJ homolog subfamily B member 6

Names: DnaJ (Hsp40) homolog, subfamily B, member 6; heat shock protein J2; mammalian relative of DnaJ

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001037940.4 → NP_001033029.1 dnaJ homolog subfamily B member 6 isoform a

See identical proteins and their annotated locations for NP_001033029.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

AK032039, AK153091, CJ079100

Consensus CDS

CCDS19151.1

UniProtKB/Swiss-Prot

O54946, Q3TE94, Q3U6L0, Q3UNJ5, Q3UYT7, Q99LA5, Q9QYI9

Related

ENSMUSP00000008733.9, ENSMUST00000008733.15

Conserved Domains (2) summary

COG0484
Location:2 → 138

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

pfam00226
Location:3 → 66

DnaJ; DnaJ domain
NM_001037941.3 → NP_001033030.2 dnaJ homolog subfamily B member 6 isoform b

See identical proteins and their annotated locations for NP_001033030.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR when compared to variant 1. Variant 2 encodes isoform b, which has a shorter, distinct C-terminus when compared to isoform a.

Source sequence(s)

BF660899, CJ079100, DV660970

Consensus CDS

CCDS19152.1

UniProtKB/TrEMBL

G3X8S5, Q9DAN5

Related

ENSMUSP00000012734.6, ENSMUST00000012734.10

Conserved Domains (2) summary

COG0484
Location:2 → 107

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

pfam00226
Location:3 → 66

DnaJ; DnaJ domain
NM_001127367.1 → NP_001120839.1 dnaJ homolog subfamily B member 6 isoform d

See identical proteins and their annotated locations for NP_001120839.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 3' coding region and 3' UTR when compared to variant 1. Variant 4 encodes isoform d, which has a shorter, distinct C-terminus when compared to isoform a.

Source sequence(s)

AK134387, BG800394, CJ079100

UniProtKB/TrEMBL

A0A0G2JER9

Conserved Domains (1) summary

pfam00226
Location:3 → 66

DnaJ; DnaJ domain
NM_001359198.1 → NP_001346127.1 dnaJ homolog subfamily B member 6 isoform e

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (e) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AC122782

UniProtKB/TrEMBL

A0A0G2JER9

Conserved Domains (1) summary

cl28246
Location:3 → 138

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]
NM_001378835.1 → NP_001365764.1 dnaJ homolog subfamily B member 6 isoform f

Status: VALIDATED

Source sequence(s)

AC122782

Conserved Domains (1) summary

COG0484
Location:2 → 138

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]
NM_011847.4 → NP_035977.2 dnaJ homolog subfamily B member 6 isoform c

See identical proteins and their annotated locations for NP_035977.2

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR when compared to variant 1. Variant 3 encodes isoform c, which has a shorter, distinct C-terminus when compared to isoform a.

Source sequence(s)

AK134387, CJ079100

Consensus CDS

CCDS39043.1

UniProtKB/TrEMBL

A0A0G2JER9

Related

ENSMUSP00000110488.2, ENSMUST00000114839.8

Conserved Domains (1) summary

pfam00226
Location:3 → 66

DnaJ; DnaJ domain