E2f2 E2F transcription factor 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: E2f2provided by FlyBase
Official Full Name: E2F transcription factor 2provided by FlyBase
Primary source: FLYBASE:FBgn0024371
Locus tag: Dmel_CG1071
See related: AllianceGenome:FB:FBgn0024371
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG1071; dE2F-2; de2f2; dE2f2; dE2F2; Dmel\CG1071; E2f; E2F; e2f2; E2F2
Summary: Enables RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Contributes to DNA-binding transcription repressor activity, RNA polymerase II-specific. Involved in several processes, including DNA endoreduplication; negative regulation of G1/S transition of mitotic cell cycle; and negative regulation of nucleobase-containing compound metabolic process. Located in chromatin. Part of DRM complex; Myb complex; and Rb-E2F complex. Is active in nucleus. Is expressed in several structures, including Malpighian tubule primordium; anterior endoderm; embryonic/larval midgut primordium; ganglia; and gut section. Human ortholog(s) of this gene implicated in prostate carcinoma. Orthologous to human E2F5 (E2F transcription factor 5). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 39A5-39A6; 2-54 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (21154293..21156749, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (21154293..21156749, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E2F directly regulates the expression of late myogenic genes, with E2f1 providing the major contribution compared with E2f2.
Title: E2F function in muscle growth is necessary and sufficient for viability in Drosophila.
The dE2F2 protein repress the expression of buffy, the anti-apoptotic member of the Bcl-2 family and activate the expression of an RNA-binding protein that destabilizes diap1 in Drosophila.
Title: The pro-apoptotic activity of Drosophila Rbf1 involves dE2F2-dependent downregulation of diap1 and buffy mRNA.
Heterozygous mutation of mip120 or E2f2 suppressed the binucleate cell phenotype in the peripodial epithelium of Myb-null wing discs.
Title: The complex containing Drosophila Myb and RB/E2F2 regulates cytokinesis in a histone H2Av-dependent manner.
dE2F2, as well as the net E2F activity, which can be depleted by mutating the common cofactor, dDp, is inhibitory for p53-independent apoptosis.
Title: E2F1 and E2F2 have opposite effects on radiation-induced p53-independent apoptosis in Drosophila.
Combined inactivation of dE2F1 and dE2F2 had no significant effect on cell division of Hippo pathway mutant cells.
Title: Context-dependent requirement for dE2F during oncogenic proliferation.
Thease results demonstrate epigenetic regulation of gene expression by Myb, and E2F2-RBF in vivo, and also provide an explanation for the ability of Mip130-null mutants to rescue the lethality of Myb-null mutants in vivo.
Title: Epigenetic regulation of gene expression by Drosophila Myb and E2F2-RBF via the Myb-MuvB/dREAM complex.
cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression
Title: G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression.
examination of linking together the Myb and E2F2 complexes in higher-order assembly to specific chromosomal sites for the regulation of transcription
Title: Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex.
RBF1 and RBF2 interact with different subsets of E2F proteins; this enables the RBF proteins to regulate E2F-dependent transcription in distinct ways
Title: Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2.
RBF2 has a unique function in repressing E2F-regulated differentiation markers and dE2F2 and RBF2 are required to regulate different sets of target genes in different tissues
Title: Retinoblastoma family 2 is required in vivo for the tissue-specific repression of dE2F2 target genes.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
contributes_to DNA-binding transcription repressor activity, RNA polymerase II-specific	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA endoreduplication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA endoreduplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G1/S transition of mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in endomitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in imaginal disc development	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of G1/S transition of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	TAS Traceable Author Statement more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of DRM complex	IDA Inferred from Direct Assay more info	PubMed
part_of Myb complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase II transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Rb-E2F complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E2F transcription factor 2

Names: CG1071-PA; CG1071-PB; E2f2-PA; E2f2-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

21154293..21156749 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001299186.1 → NP_001286115.1 E2F transcription factor 2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286115.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O77051

UniProtKB/TrEMBL

X2J9B3

Conserved Domains (2) summary

pfam02319
Location:77 → 138

E2F_TDP; E2F/DP family winged-helix DNA-binding domain

cl22411
Location:159 → 218

E2F_DD; Dimerization domain of E2F transcription factors
NM_058007.5 → NP_477355.1 E2F transcription factor 2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477355.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O77051

UniProtKB/TrEMBL

X2J9B3

Related

FBpp0081029

Conserved Domains (2) summary

pfam02319
Location:77 → 138

E2F_TDP; E2F/DP family winged-helix DNA-binding domain

cl22411
Location:159 → 218

E2F_DD; Dimerization domain of E2F transcription factors