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    gukh GUK-holder [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 53563, updated on 9-Dec-2024

    Summary

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    Official Symbol
    gukhprovided by FlyBase
    Official Full Name
    GUK-holderprovided by FlyBase
    Primary source
    FLYBASE:FBgn0026239
    Locus tag
    Dmel_CG31043
    See related
    AllianceGenome:FB:FBgn0026239
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    anon-WO0118547.205; CG14287; CG14288; CG31043; CG5456; CG6003; Dmel\CG31043; Gukh; GukH; GUKh; GUKH
    Summary
    Enables protein-macromolecule adaptor activity. Involved in protein localization. Located in terminal bouton. Is active in apical cortex. Colocalizes with presynaptic membrane. Is expressed in several structures, including anlage in statu nascendi; ganglia; gut section; head epidermis primordium; and mesoderm. Human ortholog(s) of this gene implicated in Nance-Horan syndrome and cataract 40. Orthologous to human NHS (NHS actin remodeling regulator). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See gukh in Genome Data Viewer
    Location:
    91E2-91E3; 3-65 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (18984025..19022436)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (14809747..14848158)

    Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene Smu1 spliceosomal factor Neighboring gene small ribonucleoprotein particle U1 subunit C Neighboring gene deoxyribonucleoside kinase Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Convergent and divergent ligand specificity among PDZ domains of the LAP and zonula occludens (ZO) families. Zhang Y, et al. J Biol Chem, 2006 Aug 4. PMID 16737968
    2. Allosteric control of regulated scaffolding in membrane-associated guanylate kinases. Marcette J, et al. Biochemistry, 2009 Oct 27. PMID 19769408, Free PMC Article
    3. Drosophila melanogaster Guk-holder interacts with the Scribbled PDZ1 domain and regulates epithelial development with Scribbled and Discs Large. Caria S, et al. J Biol Chem, 2018 Mar 23. PMID 29378849, Free PMC Article
    4. Interdomain interactions in the tumor suppressor discs large regulate binding to the synaptic protein GukHolder. Qian Y, et al. J Biol Chem, 2006 Nov 24. PMID 16982606, Free PMC Article
    5. Dishevelled binds the Discs large 'Hook' domain to activate GukHolder-dependent spindle positioning in Drosophila. Garcia JD, et al. PLoS One, 2014. PMID 25461409, Free PMC Article

    See all (52) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. findings reveal that Scrib's PDZ1 domain functions in the interaction with Gukh and that the Scrib-Gukh interaction has a key role in epithelial tissue development in Drosophila
      Title: Drosophila melanogaster Guk-holder interacts with the Scribbled PDZ1 domain and regulates epithelial development with Scribbled and Discs Large.
    2. The authors find that the tumor suppressor Discs large (Dlg) links the Par complex component atypical Protein Kinase C (aPKC) to the essential spindle orientation factor GukHolder (GukH).
      Title: Activation of Discs large by aPKC aligns the mitotic spindle to the polarity axis during asymmetric cell division.
    3. multiple interdomain interactions in the scaffold Discs Large (Dlg) regulate binding to the synaptic protein GukHolder (GukH)
      Title: Interdomain interactions in the tumor suppressor discs large regulate binding to the synaptic protein GukHolder.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables protein kinase A regulatory subunit binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein-macromolecule adaptor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-macromolecule adaptor activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in actin cytoskeleton organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in actin cytoskeleton organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in establishment of epithelial cell polarity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in protein localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in apical cortex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    colocalizes_with presynaptic membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in terminal bouton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    GUK-holder
    Names
    CG31043-PA
    CG31043-PB
    CG31043-PC
    CG31043-PE
    GUK holder
    gukh-PA
    gukh-PB
    gukh-PC
    gukh-PE
    gukholder

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033777.3 Reference assembly

      Range
      18984025..19022436
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_169845.2 → NP_732393.1  GUK-holder, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_732393.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9VE13, Q9VE14
      Related
      FBpp0088709
      Conserved Domains (1) summary
      pfam15273
      Location:818 → 846
      NHS; NHS-like

    2. NM_169846.2 → NP_732394.1  GUK-holder, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_732394.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q1LZ39, Q9VE16
      Related
      FBpp0088708

    3. NM_001032021.2 → NP_001027192.1  GUK-holder, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001027192.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q4AB30

    4. NM_001104364.2 → NP_001097834.2  GUK-holder, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001097834.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      A8JR42
      Conserved Domains (1) summary
      pfam15273
      Location:818 → 846
      NHS; NHS-like
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A8JR42 A8JR42
    Q4AB30 Q4AB30
    Q95S72 Q95S72
    Q9VE13 Q9VE13
    Q9VE16 Q9VE16

    Gene LinkOut

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