Eed embryonic ectoderm development [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Eedprovided by MGI
Official Full Name: embryonic ectoderm developmentprovided by MGI
Primary source: MGI:MGI:95286
See related: Ensembl:ENSMUSG00000030619 AllianceGenome:MGI:95286
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: lusk; l7Rn5; l(7)5Rn
Summary: Enables chromatin binding activity; enzyme activator activity; and methylated histone binding activity. Involved in facultative heterochromatin formation. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; regulation of adaxial/abaxial pattern formation; and regulation of gene expression. Located in pronucleus and sex chromatin. Part of ESC/E(Z) complex and chromatin silencing complex. Is expressed in several structures, including branchial arch; central nervous system; early embryo; embryo mesenchyme; and genitourinary system. Orthologous to human EED (embryonic ectoderm development). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 15.5), liver E14 (RPKM 11.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 D3; 7 50.42 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (89603862..89630184, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (89954654..89980976, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The epigenetic state of EED-Gli3-Gli1 regulatory axis controls embryonic cortical neurogenesis.
Title: The epigenetic state of EED-Gli3-Gli1 regulatory axis controls embryonic cortical neurogenesis.
EED is required for mouse primordial germ cell differentiation in the embryonic gonad.
Title: EED is required for mouse primordial germ cell differentiation in the embryonic gonad.
these findings established a critical role of EED in the development of hippocampal dentate gyrus, which might shed new light on the molecular mechanism of intellectual disability in patients with EED mutations.
Title: Polycomb Protein EED Regulates Neuronal Differentiation through Targeting SOX11 in Hippocampal Dentate Gyrus.
In maternal-null (Eed(m-/-)) but not zygotic-null (Eed(-/-)) early embryos, the maternal X-chromosome ectopically induced Xist and underwent inactivation. Expression of PRC2 in the oocyte and transmission of the gene products to the embryo may dictate the occurrence of imprinted X-inactivation in mammals.
Title: Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2.
These findings identify Eed/PRC2 as necessary for maintenance of global gene silencing and terminal differentiation in b cells, and suggest a ''two-hit'' (chromatin and hyperglycemia) model of b cell dedifferentiation.
Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes.
These data reveal a novel epigenetic regulated pathway and suggest an essential role for Eed in SVZ homeostasis and injury.
Title: Polycomb Protein Eed is Required for Neurogenesis and Cortical Injury Activation in the Subventricular Zone.
Eed, an essential component of the Polycomb group complex 2 (PRC2), is required for establishing H3K27me3 imprinting
Title: Maternal Eed knockout causes loss of H3K27me3 imprinting and random X inactivation in the extraembryonic cells.
The mouse nerves with EED-deficient Schwann cells display slow axonal regeneration with significantly low expression of axon guidance genes.
Title: Polycomb repression regulates Schwann cell proliferation and axon regeneration after nerve injury.
Using a genetic approach that deleted Eed specifically in the growing oocyte, results showed that oocytes lacked H3K27me3 and produced offspring with significant overgrowth that involved increased adiposity (percentage fat) and increased bone mineral density. These data demonstrate that EED is required in the oocyte for programming developmental outcomes that affect life-long outcomes in offspring.
Title: Loss of maternal EED results in postnatal overgrowth.
Eed, an essential subunit of PRC2, is crucial to maintain the identity of CD4(+) T cells under TGFbeta-induced regulatory T cell (Treg)-polarizing conditions.
Title: Loss of Eed leads to lineage instability and increased CD8 expression of mouse CD4(+) T cells upon TGFβ signaling.

Submit: New GeneRIF Correction See all GeneRIFs (44)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (4) 1 citation
Endonuclease-mediated (2)
Targeted (12) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables enzyme activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables enzyme activator activity	ISO Inferred from Sequence Orthology more info
enables enzyme activator activity	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables methylated histone binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to nucleosome binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sphingomyelin phosphodiesterase activator activity	ISO Inferred from Sequence Orthology more info	PubMed
enables transcription corepressor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in facultative heterochromatin formation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within genomic imprinting	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within oligodendrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of amide metabolic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of lipid metabolic process	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within regulation of adaxial/abaxial pattern formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of membrane lipid metabolic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info	PubMed

Component	Evidence Code	Pubs
part_of ESC/E(Z) complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ESC/E(Z) complex	IDA Inferred from Direct Assay more info	PubMed
part_of ESC/E(Z) complex	ISO Inferred from Sequence Orthology more info
part_of chromatin silencing complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in pronucleus	IDA Inferred from Direct Assay more info	PubMed
located_in sex chromatin	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: polycomb protein EED

Names: lethal, Chr 7, Rinchik 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021876.3 → NP_068676.1 polycomb protein EED

See identical proteins and their annotated locations for NP_068676.1

Status: VALIDATED

Source sequence(s)

AK131976, BQ899891, CJ200028, U97675

Consensus CDS

CCDS40016.1

UniProtKB/Swiss-Prot

P97462, Q921E6

UniProtKB/TrEMBL

A0A5F8MPX8

Related

ENSMUSP00000159149.2, ENSMUST00000238981.2

Conserved Domains (2) summary

sd00039
Location:96 → 140

7WD40; WD40 repeat [structural motif]

cl29593
Location:150 → 438

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...