Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkaa1provided by MGI
Official Full Name: protein kinase, AMP-activated, alpha 1 catalytic subunitprovided by MGI
Primary source: MGI:MGI:2145955
See related: Ensembl:ENSMUSG00000050697 AllianceGenome:MGI:2145955
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: AMPKalpha1; C130083N04Rik
Summary: Enables AMP-activated protein kinase activity; chromatin binding activity; and histone H2BS36 kinase activity. Involved in several processes, including negative regulation of TORC1 signaling; regulation of organelle organization; and regulation of primary metabolic process. Acts upstream of or within several processes, including cellular response to prostaglandin E stimulus; positive regulation of skeletal muscle tissue development; and regulation of peptidyl-serine phosphorylation. Located in several cellular components, including axon; dendrite; and neuronal cell body. Is active in chromatin. Is expressed in several structures, including brain; dorsal root ganglion; early conceptus; liver; and spinal cord. Human ortholog(s) of this gene implicated in Huntington's disease; breast cancer; and colon cancer. Orthologous to human PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in bladder adult (RPKM 5.3), limb E14.5 (RPKM 5.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 A1; 15 1.99 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (5172641..5211380)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (5143861..5181899)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hydrogen sulfide inhibits skeletal muscle ageing by up-regulating autophagy through promoting deubiquitination of adenosine 5'-monophosphate (AMP)-activated protein kinase alpha1 via ubiquitin specific peptidase 5.
Title: Hydrogen sulfide inhibits skeletal muscle ageing by up-regulating autophagy through promoting deubiquitination of adenosine 5'-monophosphate (AMP)-activated protein kinase α1 via ubiquitin specific peptidase 5.
MLKL promotes hepatocarcinogenesis through inhibition of AMPK-mediated autophagy.
Title: MLKL promotes hepatocarcinogenesis through inhibition of AMPK-mediated autophagy.
SLC7A11-ROS/alphaKG-AMPK axis regulates liver inflammation through mitophagy and impairs liver fibrosis and NASH progression.
Title: SLC7A11-ROS/αKG-AMPK axis regulates liver inflammation through mitophagy and impairs liver fibrosis and NASH progression.
Clostridium butyricum upregulates GPR109A/AMPK/PGC-1alpha and ameliorates acute pancreatitis-associated intestinal barrier injury in mice.
Title: Clostridium butyricum upregulates GPR109A/AMPK/PGC-1α and ameliorates acute pancreatitis-associated intestinal barrier injury in mice.
Loss of AMPK activity induces organelle dysfunction and oxidative stress during oocyte aging.
Title: Loss of AMPK activity induces organelle dysfunction and oxidative stress during oocyte aging.
Skeletal muscle-specific inducible AMPKalpha1/alpha2 knockout mice develop muscle weakness, glycogen depletion, and fibrosis that persists during disuse atrophy.
Title: Skeletal muscle-specific inducible AMPKα1/α2 knockout mice develop muscle weakness, glycogen depletion, and fibrosis that persists during disuse atrophy.
Mitigation of aircraft noise-induced vascular dysfunction and oxidative stress by exercise, fasting, and pharmacological alpha1AMPK activation: molecular proof of a protective key role of endothelial alpha1AMPK against environmental noise exposure.
Title: Mitigation of aircraft noise-induced vascular dysfunction and oxidative stress by exercise, fasting, and pharmacological α1AMPK activation: molecular proof of a protective key role of endothelial α1AMPK against environmental noise exposure.
Diet-inducing hypercholesterolemia show decreased O-GlcNAcylation of liver proteins through modulation of AMPK.
Title: Diet-inducing hypercholesterolemia show decreased O-GlcNAcylation of liver proteins through modulation of AMPK.
AMPKalpha1 negatively regulates osteoclastogenesis and mitigates pathological bone loss.
Title: AMPKα1 negatively regulates osteoclastogenesis and mitigates pathological bone loss.
Constitutively activated AMPKalpha1 protects against skeletal aging in mice by promoting bone-derived IGF-1 secretion.
Title: Constitutively activated AMPKα1 protects against skeletal aging in mice by promoting bone-derived IGF-1 secretion.

Submit: New GeneRIF Correction See all GeneRIFs (362)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (7)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH135932 (e-PCR)
- UniSTS:209929

RH125513 (e-PCR)
- UniSTS:162330

AI450832 (e-PCR)
- UniSTS:160433

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables AMP-activated protein kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables AMP-activated protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables AMP-activated protein kinase activity	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info
enables tau-protein kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in CAMKK-AMPK signaling cascade	IEA Inferred from Electronic Annotation more info
involved_in CAMKK-AMPK signaling cascade	ISO Inferred from Sequence Orthology more info
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in cellular response to calcium ion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in cellular response to ethanol	IEA Inferred from Electronic Annotation more info
involved_in cellular response to glucose starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glucose starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to glucose starvation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to glucose stimulus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hydrogen peroxide	IEA Inferred from Electronic Annotation more info
involved_in cellular response to hypoxia	IEA Inferred from Electronic Annotation more info
involved_in cellular response to nutrient levels	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to nutrient levels	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nutrient levels	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to oxidative stress	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular response to prostaglandin E stimulus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info
involved_in cholesterol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in cold acclimation	IEA Inferred from Electronic Annotation more info
involved_in cold acclimation	ISO Inferred from Sequence Orthology more info
involved_in energy homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in energy homeostasis	ISO Inferred from Sequence Orthology more info
involved_in energy homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in fatty acid homeostasis	ISO Inferred from Sequence Orthology more info
involved_in fatty acid homeostasis	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within fatty acid oxidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glucose homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glucose metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in lipid droplet disassembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_positive_effect lipid droplet disassembly	ISO Inferred from Sequence Orthology more info
involved_in motor behavior	IEA Inferred from Electronic Annotation more info
involved_in motor behavior	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of TOR signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of TOR signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TORC1 signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of hepatocyte apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of hepatocyte apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of insulin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of lipid catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of tubulin deacetylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron cellular homeostasis	IEA Inferred from Electronic Annotation more info
involved_in neuron cellular homeostasis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of T cell activation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of T cell mediated immune response to tumor cell	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of adipose tissue development	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glycolytic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of mitochondrial transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of peptidyl-lysine acetylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein targeting to mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of skeletal muscle tissue development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to lipid droplet	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to lipid droplet	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of microtubule cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of peptidyl-serine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of stress granule assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of vesicle-mediated transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of vesicle-mediated transport	ISO Inferred from Sequence Orthology more info
involved_in response to UV	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to activity	IEA Inferred from Electronic Annotation more info
involved_in response to activity	ISO Inferred from Sequence Orthology more info
involved_in response to caffeine	IEA Inferred from Electronic Annotation more info
involved_in response to caffeine	ISO Inferred from Sequence Orthology more info
involved_in response to estrogen	IEA Inferred from Electronic Annotation more info
involved_in response to gamma radiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hydrogen peroxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in axon	IGI Inferred from Genetic Interaction more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite	IGI Inferred from Genetic Interaction more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IGI Inferred from Genetic Interaction more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
part_of nucleotide-activated protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nucleotide-activated protein kinase complex	ISO Inferred from Sequence Orthology more info
part_of nucleotide-activated protein kinase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 5'-AMP-activated protein kinase catalytic subunit alpha-1

Names: ACACA kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMPK subunit alpha-1; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; tau-protein kinase PRKAA1

NP_001013385.3

EC 2.7.11.1

EC 2.7.11.26

EC 2.7.11.31

NP_001342569.1

EC 2.7.11.1

EC 2.7.11.26

EC 2.7.11.31

XP_036014946.1

EC 2.7.11.1

EC 2.7.11.26

EC 2.7.11.31

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001013367.3 → NP_001013385.3 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1

See identical proteins and their annotated locations for NP_001013385.3

Status: VALIDATED

Source sequence(s)

AC135079, AK081874, AK160612, BY134921, CB245934

Consensus CDS

CCDS49574.1

UniProtKB/Swiss-Prot

Q5EG47

UniProtKB/TrEMBL

Q3TUQ7

Related

ENSMUSP00000063166.9, ENSMUST00000051186.9

Conserved Domains (4) summary

cd12199
Location:404 → 557

AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit

smart00220
Location:27 → 279

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14403
Location:296 → 360

UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)

cd14079
Location:24 → 279

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase
NM_001355640.1 → NP_001342569.1 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 2

Status: VALIDATED

Source sequence(s)

AC135079

Conserved Domains (3) summary

cd12199
Location:260 → 413

AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit

cd14403
Location:152 → 216

UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)

cl21453
Location:1 → 135

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

5172641..5211380

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036159053.1 → XP_036014946.1 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1

UniProtKB/TrEMBL

Q3TUQ7

Conserved Domains (3) summary

cd12199
Location:434 → 587

AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit

cd14403
Location:326 → 390

UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)

cd14079
Location:24 → 309

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase