Pak3 Pak3 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Pak3provided by FlyBase
Official Full Name: Pak3provided by FlyBase
Primary source: FLYBASE:FBgn0044826
Locus tag: Dmel_CG14895
See related: AllianceGenome:FB:FBgn0044826
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0118547.285; CG14895; D-Pak3; Dmel\CG14895; DmPAK3; dpak3; dPAK3; Dpak3; DPak3; DPAK3; Pak; PAK-3; pak3; PaK3; PAK3
Summary: Enables protein serine/threonine kinase activity and small GTPase binding activity. Involved in several processes, including border follicle cell migration; myoblast fusion; and positive regulation of synapse assembly. Located in cytoplasm and ruffle. Is expressed in antennal disc; embryonic dorsal epidermis; embryonic/larval lymph gland; embryonic/larval salivary gland; and fusion competent cell. Human ortholog(s) of this gene implicated in breast cancer; ductal carcinoma in situ; non-syndromic X-linked intellectual disability 30; renal cell carcinoma; and schizophrenia. Orthologous to human PAK1 (p21 (RAC1) activated kinase 1); PAK2 (p21 (RAC1) activated kinase 2); and PAK3 (p21 (RAC1) activated kinase 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 89B16-89B17; 3-58 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (16445007..16453821, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (12270729..12279543, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study reports the role of the protein kinase Pak3 in maintaining apical-basal polarity in migrating border cell clusters during Drosophila oogenesis. Pak3 is enriched in border cells and downregulation of its function impedes border cell movement.
Title: Pak3 regulates apical-basal polarity in migrating border cells during Drosophila oogenesis.
The DPak3 is enriched at the site of fusion colocalizing with the F-actin focus within a podosome-like structure (PLS), and promotes actin filament assembly during PLS invasion.
Title: Group I PAKs function downstream of Rac to promote podosome invasion during myoblast fusion in vivo.
These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.
Title: Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing.
pak3 therefore regulates synapse development in vivo, and when mutated, suppresses the synaptic defects that result from spastin loss.
Title: Loss of Drosophila melanogaster p21-activated kinase 3 suppresses defects in synapse structure and function caused by spastin mutations.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0423 (e-PCR)
- UniSTS:34580

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell projection organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in myoblast fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of synapse assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of axonogenesis	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Pak3

Names: CG14895-PA; CG14895-PC; CG14895-PD; Pak3-PA; Pak3-PC; Pak3-PD; p21-activated kinase 3

NP_001262638.1

EC 2.7.11.1

NP_650545.2

EC 2.7.11.1

NP_732141.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

16445007..16453821 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001275709.1 → NP_001262638.1 Pak3, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262638.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KGS4, Q8IGZ0

Conserved Domains (3) summary

smart00220
Location:293 → 545

S_TKc; Serine/Threonine protein kinases, catalytic domain

pfam00786
Location:19 → 73

PBD; P21-Rho-binding domain

cl21453
Location:288 → 545

PKc_like; Protein Kinases, catalytic domain
NM_169719.3 → NP_732141.2 Pak3, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_732141.2

Status: REVIEWED

UniProtKB/TrEMBL

Q8IGZ0, Q8INB3, Q9VEV1

Conserved Domains (3) summary

smart00220
Location:293 → 545

S_TKc; Serine/Threonine protein kinases, catalytic domain

pfam00786
Location:19 → 73

PBD; P21-Rho-binding domain

cl21453
Location:288 → 545

PKc_like; Protein Kinases, catalytic domain
NM_142288.4 → NP_650545.2 Pak3, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_650545.2

Status: REVIEWED

UniProtKB/TrEMBL

Q8IGZ0, Q8INB3, Q9VEV1

Related

FBpp0082763

Conserved Domains (3) summary

smart00220
Location:293 → 545

S_TKc; Serine/Threonine protein kinases, catalytic domain

pfam00786
Location:19 → 73

PBD; P21-Rho-binding domain

cl21453
Location:288 → 545

PKc_like; Protein Kinases, catalytic domain