CalpA Calpain-A [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: CalpAprovided by FlyBase
Official Full Name: Calpain-Aprovided by FlyBase
Primary source: FLYBASE:FBgn0012051
Locus tag: Dmel_CG7563
See related: AllianceGenome:FB:FBgn0012051
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: calpA; CG18152; CG7563; Dmel\CG7563; EST C
Summary: Enables calcium-dependent cysteine-type endopeptidase activity. Involved in several processes, including determination of adult lifespan; neuron remodeling; and protein autoprocessing. Located in actin cytoskeleton and neuronal cell body. Is active in Golgi apparatus and endoplasmic reticulum. Is expressed in several structures, including adult central nervous system; adult head; gut section; head epidermis primordium; and neuromere. Used to study intellectual disability and obesity. Human ortholog(s) of this gene implicated in autosomal dominant limb-girdle muscular dystrophy; autosomal recessive limb-girdle muscular dystrophy type 2A; muscular dystrophy; and stomach cancer. Orthologous to several human genes including CAPN9 (calpain 9). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 56D5-56D5; 2-89 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (19425113..19430564)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (15312618..15318069)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Here we describe the steps for tissue preparation and calpain activity measurement in the Drosophila embryo.
Title: Expression and Activity of Calpain A in Drosophila melanogaster.
Calpain A functions as part of a timing module in mitosis, at the interface between calcium signals and mitotic cycles of the Drosophila embryo.
Title: Calpain A controls mitotic synchrony in the Drosophila blastoderm embryo.
CalpA targets free Cactus, which is incorporated into and modulates Toll-responsive complexes in the embryo and immune system.
Title: Calpain A modulates Toll responses by limited Cactus/IκB proteolysis.
Study demonstrates that CalpB but not the structurally related CalpA has an important modulator function during in vivo cell migration.
Title: CalpB modulates border cell migration in Drosophila egg chambers.
Data show that mutations that disrupted endogenous calpainA or calpainB activity in transgenic flies suppressed tau toxicity.
Title: Implicating calpain in tau-mediated toxicity in vivo.
Dpp signals increase Cactus levels through Calpain A inhibition, thereby interfering with Dorsal activation
Title: The Ca2+-dependent protease Calpain A regulates Cactus/I kappaB levels during Drosophila development in response to maternal Dpp signals.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	NAS Non-traceable Author Statement more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-dependent cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cuticle development	IDA Inferred from Direct Assay more info	PubMed
involved_in defense response to fungus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal/ventral pattern formation	IDA Inferred from Direct Assay more info	PubMed
involved_in larval locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in myoblast fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron remodeling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein autoprocessing	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Calpain-A

Names: CG7563-PA; CG7563-PB; CG7563-PC; CG7563-PD; CalpA-PA; CalpA-PB; CalpA-PC; CalpA-PD; calpain; calpain A; calpain lacking calmodulin-like domain; calpainA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

19425113..19430564

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001299684.1 → NP_001286613.1 Calpain-A, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286613.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LG26

Conserved Domains (3) summary

cd00051
Location:719 → 765

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00648
Location:104 → 400

Peptidase_C2; Calpain family cysteine protease

pfam01067
Location:418 → 565

Calpain_III; Calpain large subunit, domain III
NM_001103908.2 → NP_001097378.1 Calpain-A, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097378.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q11002, Q9V8U6, Q9V8U7

UniProtKB/TrEMBL

A8DYJ3

Conserved Domains (3) summary

cd00214
Location:401 → 554

Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...

cd16196
Location:585 → 828

EFh_PEF_CalpA_B; Penta-EF hand, calcium binding motifs, found in Drosophila melanogaster calpain-A (CalpA), calpain-B (CalpB), and similar proteins

pfam00648
Location:89 → 385

Peptidase_C2; Calpain family cysteine protease
NM_057699.5 → NP_477047.1 Calpain-A, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477047.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q11002, Q9V8U6, Q9V8U7

UniProtKB/TrEMBL

A8DYJ3

Related

FBpp0085714

Conserved Domains (3) summary

cd00214
Location:401 → 554

Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...

cd16196
Location:585 → 828

EFh_PEF_CalpA_B; Penta-EF hand, calcium binding motifs, found in Drosophila melanogaster calpain-A (CalpA), calpain-B (CalpB), and similar proteins

pfam00648
Location:89 → 385

Peptidase_C2; Calpain family cysteine protease
NM_057700.4 → NP_477048.1 Calpain-A, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477048.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q11002

Related

FBpp0085715

Conserved Domains (2) summary

pfam00648
Location:89 → 385

Peptidase_C2; Calpain family cysteine protease

pfam01067
Location:403 → 550

Calpain_III; Calpain large subunit, domain III