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    TCB2 tricalbin [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855637, updated on 9-Dec-2024

    Summary

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    Official Symbol
    TCB2
    Official Full Name
    tricalbin
    Primary source
    SGD:S000005031
    Locus tag
    YNL087W
    See related
    AllianceGenome:SGD:S000005031; FungiDB:YNL087W; VEuPathDB:YNL087W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables lipid binding activity. Involved in endoplasmic reticulum membrane organization and regulation of phosphatidylinositol dephosphorylation. Acts upstream of or within ER to Golgi ceramide transport and phospholipid homeostasis. Located in cellular bud and cortical endoplasmic reticulum. Orthologous to human ESYT1 (extended synaptotagmin 1) and ESYT2 (extended synaptotagmin 2). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See TCB2 in Genome Data Viewer
    Location:
    chromosome: XIV
    Exon count:
    1
    Sequence:
    Chromosome: XIV; NC_001146.8 (462411..465947)

    Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene Rho family GTPase RHO2 Neighboring gene DNA topoisomerase 2 Neighboring gene Snn1p Neighboring gene Mkt1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001146.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae. Brennan TC, et al. Appl Environ Microbiol, 2015 May 15. PMID 25746998, Free PMC Article
    2. ER-plasma membrane contact sites deliver ER lipids and proteins for rapid cell surface expansion. Smith M, et al. J Cell Biol, 2024 Dec 2. PMID 39302311,
    3. Beta-strand recombination in tricalbin evolution and the origin of synaptotagmin-like C2 domains. Jiménez JL, et al. Proteins, 2007 Aug 15. PMID 17510957
    4. The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family. Schulz TA, et al. Biochemistry, 2004 Apr 6. PMID 15049706
    5. Tricalbin proteins regulate plasma membrane phospholipid homeostasis. Thomas FB, et al. Life Sci Alliance, 2022 Aug. PMID 35440494, Free PMC Article

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Tricalbins function as multimers in membrane-trafficking events. TCB2 localizes first to the cell surface, and then to intracellular vesicles near the vacuole. tcb2 mutants have altered vacuole morphology and are hypersensitive to cyclohexamide.
      Title: Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking.
    2. TCB2 likely does not bind lipids in response to calcium signaling. Suggested that calcium-insensitive TCB2 may mediate the creation of hetero-oligomeric tricalbin complexes in which TCB1, TCB3, or both supply a calcium-dependent membrane binding activity.
      Title: The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family.
    3. GFP-fusion protein migrates from the cell surface to intracellular vesices near the vacuole. Binds the related proteins Tcb1 and Tcb3. Binds Pdr1p Knockout phenotype: Hypersensitivity to cycloheximide; Suppressed by RSP5.
      Title: Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables lipid binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables lipid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in ER to Golgi ceramide transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within ER to Golgi ceramide transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endoplasmic reticulum membrane organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in endoplasmic reticulum membrane organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in endoplasmic reticulum-plasma membrane tethering IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within phospholipid homeostasis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of phosphatidylinositol dephosphorylation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell periphery HDA PubMed 
    located_in cellular bud IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cortical endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum HDA PubMed 
    is_active_in endoplasmic reticulum IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in intracellular membrane-bounded organelle IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    tricalbin
    NP_014312.1
    • Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane (PM); regulates PI4P levels by controlling access of Sac1p to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; contains 3 calcium and lipid binding domains; localizes to the cortical ER, the cell periphery and is enriched in bud membranes; mRNA is targeted to the bud tip

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001146.8 Reference assembly

      Range
      462411..465947
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182925.1 → NP_014312.1  TPA: tricalbin [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014312.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W192, P48231, Q45TY9
      UniProtKB/TrEMBL
      A6ZRZ3, N1NWL1
      Conserved Domains (1) summary
      COG5038
      Location:4 → 1178
      COG5038; Ca2+-dependent lipid-binding protein, contains C2 domain [General function prediction only]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P48231.1

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