glh-1 ATP-dependent RNA helicase glh-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: glh-1
Official Full Name: ATP-dependent RNA helicase glh-1
Primary source: WormBase:WBGene00001598
Locus tag: CELE_T21G5.3
See related: AllianceGenome:WB:WBGene00001598
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables DEAD/H-box RNA helicase binding activity; JUN kinase binding activity; and RNA helicase activity. Involved in germ cell development and post-embryonic development. Located in P granule. Is expressed in Z2; Z3; germ line; and gonad. Orthologous to human DDX4 (DEAD-box helicase 4). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See glh-1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 7

Sequence:: Chromosome: I; NC_003279.8 (6854478..6857536)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

we show that removal of either of two critical P-granule components, PGL-1 or GLH-1, is sufficient to cause germ cells to express UNC-119::GFP and MYO-3 and to display RNA accumulation defects similar to those observed after depletion of P granules. Our data identify P granules as critical modulators of the germline transcriptome and guardians of germ cell fate.
Title: Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline.
Data show that GLH-1 and its FG domain are not sufficient to form granules, but require factors like PGL-1 to nucleate the localized concentration of GLH proteins.
Title: P granules extend the nuclear pore complex environment in the C. elegans germ line.
the GLH-1/DCR-1 complex may function in the transport, deposition, or regulation of maternally-transcribed mRNAs and their associated miRNAs.
Title: C. elegans Dicer interacts with the P-granule component GLH-1 and both regulate germline RNPs.
KGB-1 and CSN-5 regulate GLH-1 levels, with GLH-1 targeted for proteosomal degradation by KGB-1 and stabilized by CSN-5.
Title: GLH-1, the C. elegans P granule protein, is controlled by the JNK KGB-1 and by the COP9 subunit CSN-5.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T21G5.3 (e-PCR)
- UniSTS:306285

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DEAD/H-box RNA helicase binding	IPI Inferred from Physical Interaction more info	PubMed
enables JUN kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA metabolic process	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in gamete generation	IBA Inferred from Biological aspect of Ancestor more info
involved_in germ cell development	IBA Inferred from Biological aspect of Ancestor more info
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in P granule	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ATP-dependent RNA helicase glh-1

NP_491963.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

6854478..6857536

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_059562.6 → NP_491963.1 ATP-dependent RNA helicase glh-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_491963.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P34689, Q22873, Q7KQH5, Q9TXH4

Conserved Domains (5) summary

smart00343
Location:243 → 259

ZnF_C2HC; zinc finger

smart00487
Location:359 → 561

DEXDc; DEAD-like helicases superfamily

cd00268
Location:343 → 558

DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation. The name derives from the sequence of the Walker B motif ...

pfam00098
Location:183 → 200

zf-CCHC; Zinc knuckle

pfam00271
Location:579 → 701

Helicase_C; Helicase conserved C-terminal domain