rde-1 Piwi domain-containing protein [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: rde-1
Official Full Name: Piwi domain-containing protein
Primary source: WormBase:WBGene00004323
Locus tag: CELE_K08H10.7
See related: AllianceGenome:WB:WBGene00004323
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables DEAD/H-box RNA helicase binding activity; RNA endonuclease activity, producing 5'-phosphomonoesters; and siRNA binding activity. Involved in regulatory ncRNA-mediated heterochromatin formation and regulatory ncRNA-mediated post-transcriptional gene silencing. Part of RISC complex. Is expressed in male. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See rde-1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 11

Sequence:: Chromosome: V; NC_003283.11 (9987916..9991642, complement)

Chromosome V - NC_003283.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003283.11

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results uncouple the role of the RDE-1 RNase H activity in small RNA maturation from its role in target-mRNA silencing in vivo.
Title: RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans.
The majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes.
Title: Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

K08H10.7 (e-PCR)
- UniSTS:312693

K08H10.9 (e-PCR)
- UniSTS:316038

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DEAD/H-box RNA helicase binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IMP Inferred from Mutant Phenotype more info	PubMed
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in regulatory ncRNA-mediated heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Piwi domain-containing protein

NP_741611.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003283.11 Reference assembly

Range

9987916..9991642 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_171525.7 → NP_741611.1 Piwi domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_741611.1

Status: REVIEWED

UniProtKB/TrEMBL

G5EEH0

Conserved Domains (2) summary

cd02846
Location:280 → 419

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:467 → 982

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...